TY - JOUR
T1 - The role of vitellogenin during gestation of Girardinichthys viviparus and Ameca splendens; two goodeid fish with matrotrophic viviparity
AU - Vega-López, Armando
AU - Ortiz-Ordóñez, Esperanza
AU - Uría-Galicia, Esther
AU - Mendoza-Santana, E. Laura
AU - Hernández-Cornejo, Rubí
AU - Atondo-Mexia, Roxana
AU - García-Gasca, Alejandra
AU - García-Latorre, Ethel
AU - Domínguez-López, Maria Lilia
N1 - Funding Information:
We are grateful to SEMARNAT, Dirección General de Vida Silvestre (México) for authorizing the collection of parent specimens and most particularly to biologist Gabriel Solano and Dolores Morales. Our thanks also to the staff of the Lago de Texcoco project, CNA, in particular MVZ Fernando Núñez for the support in the field. This study was financed by the Instituto Politécnico Nacional, CGPI code 20041154. Ethel García-Latorre and Maria Lilia Domínguez-López are fellows of COFAA-IPN, EDI and SNI.
PY - 2007/7
Y1 - 2007/7
N2 - Goodeid fish have matrotrophic viviparity, and unlike lecitotrophic fish, yolk loss forces the female to provide the nutritional requirements for embryonic development. Vitellogenin (VTG) is the yolk precursor protein synthesized in the maternal liver, but there is only circumstantial evidence regarding VTG supply during the ontogenesis of bony fish with matrotrophic viviparity. Therefore, the goal of the present study was to identify and quantify VTG during gestation of the black fin goodeid Girardinichthys viviparus and the butterfly split-fin goodeid Ameca splendens. Females at different gonadic developmental stages were selected in order to evaluate VTG mRNA expression in the maternal liver using RT-PCR; VTG quantification in maternal muscle and liver, as well as in the embryos, was done using ELISA, and immunohistochemical detection of VTG was done in the black fin goodeid. The results suggest that VTG supplies nutrients during embryonic development of both species, which have different life histories. It is possible that the transition from lecitotrophy to matrotrophic viviparity in bony fish with intraluminal gestation involved adaptive transition strategies that included changes in the relationship between oocytes and follicular cells, as well as a gradual loss of VTG synthesis during embryonic development.
AB - Goodeid fish have matrotrophic viviparity, and unlike lecitotrophic fish, yolk loss forces the female to provide the nutritional requirements for embryonic development. Vitellogenin (VTG) is the yolk precursor protein synthesized in the maternal liver, but there is only circumstantial evidence regarding VTG supply during the ontogenesis of bony fish with matrotrophic viviparity. Therefore, the goal of the present study was to identify and quantify VTG during gestation of the black fin goodeid Girardinichthys viviparus and the butterfly split-fin goodeid Ameca splendens. Females at different gonadic developmental stages were selected in order to evaluate VTG mRNA expression in the maternal liver using RT-PCR; VTG quantification in maternal muscle and liver, as well as in the embryos, was done using ELISA, and immunohistochemical detection of VTG was done in the black fin goodeid. The results suggest that VTG supplies nutrients during embryonic development of both species, which have different life histories. It is possible that the transition from lecitotrophy to matrotrophic viviparity in bony fish with intraluminal gestation involved adaptive transition strategies that included changes in the relationship between oocytes and follicular cells, as well as a gradual loss of VTG synthesis during embryonic development.
KW - Ameca splendens
KW - Embryonic development
KW - Girardinichthys viviparus
KW - Goodeid fish
KW - Matrotrophic viviparity
KW - Vitellogenin
UR - http://www.scopus.com/inward/record.url?scp=34248568799&partnerID=8YFLogxK
U2 - 10.1016/j.cbpa.2006.10.039
DO - 10.1016/j.cbpa.2006.10.039
M3 - Artículo
C2 - 17215155
SN - 1095-6433
VL - 147
SP - 731
EP - 742
JO - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
JF - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
IS - 3
ER -