TY - JOUR
T1 - The proteolytic system of the fission yeast Schizosaccharomyces pombe
AU - Suárez-Rendueles, Paz
AU - Villa, Lourdes
AU - Arbesú, María José
AU - Escudero, Blanca
N1 - Funding Information:
The authors are very grateful to Prof. S. Gasc6n for scientific advice. This work was supported in part by Grant PBT87-0030 from the Comisi6n Interministerial de Ciencia y Tecnolog~a of Spain. L. Villa was a fellow from the Agencia Espafiola de Cooperaci6n Internacional (Instituto de Coop-eraci6n Iberoamericana).
PY - 1991/6/15
Y1 - 1991/6/15
N2 - Proteinase and peptidase activities of the fission yeast Schizosaccharomyces pombe were investigated. Several intracellular proteolytic enzymes were found: two endoproteinases, one carboxypeptidase, one aminopeptidase and one dipeptidyl-aminopeptidase. In addition, proteinase inhibitors were detected. In fresh crude extracts an activation procedure is needed to measure maximal activities of endoproteinases and carboxypeptidase, whose level is markedly dependent on growth medium composition and on growth phase, while aminopeptidase and dipeptidyl-aminopeptidase activities are very little, if at all, regulated by the carbon source.
AB - Proteinase and peptidase activities of the fission yeast Schizosaccharomyces pombe were investigated. Several intracellular proteolytic enzymes were found: two endoproteinases, one carboxypeptidase, one aminopeptidase and one dipeptidyl-aminopeptidase. In addition, proteinase inhibitors were detected. In fresh crude extracts an activation procedure is needed to measure maximal activities of endoproteinases and carboxypeptidase, whose level is markedly dependent on growth medium composition and on growth phase, while aminopeptidase and dipeptidyl-aminopeptidase activities are very little, if at all, regulated by the carbon source.
KW - Fission yeast
KW - Heterologous gene expression
KW - Proteolytic system
KW - Schizosaccharomyces pombe
UR - http://www.scopus.com/inward/record.url?scp=0025832202&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.1991.tb04748.x
DO - 10.1111/j.1574-6968.1991.tb04748.x
M3 - Artículo
SN - 0378-1097
VL - 81
SP - 215
EP - 220
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -