The pep4 gene encoding proteinase A is involved in dimorphism and pathogenesis of Ustilago maydis

Cinthia V. Soberanes-Gutiérrez, Margarita Juárez-Montiel, Omar Olguín-Rodríguez, César Hernández-Rodríguez, José Ruiz-Herrera, Lourdes Villa-Tanaca

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Abstract

© 2015 BSPP and John Wiley & Sons Ltd. Vacuole proteases have important functions in different physiological processes in fungi. Taking this aspect into consideration, and as a continuation of our studies on the analysis of the proteolytic system of Ustilago maydis, a phytopathogenic member of the Basidiomycota, we have analysed the role of the pep4 gene encoding the vacuolar acid proteinase PrA in the pathogenesis and morphogenesis of the fungus. After confirmation of the location of the protease in the vacuole using fluorescent probes, we obtained deletion mutants of the gene in sexually compatible strains of U.maydis (FB1 and FB2), and analysed their phenotypes. It was observed that the yeast to mycelium dimorphic transition induced by a pH change in the medium, or the use of a fatty acid as sole carbon source, was severely reduced in Δpep4 mutants. In addition, the virulence of the mutants in maize seedlings was reduced, as revealed by the lower proportion of plants infected and the reduction in size of the tumours induced by the pathogen, when compared with wild-type strains. All of these phenotypic alterations were reversed by complementation of the mutant strains with the wild-type gene. These results provide evidence of the importance of the pep4 gene for the morphogenesis and virulence of U.maydis.
Original languageAmerican English
Pages (from-to)837-846
Number of pages752
JournalMolecular Plant Pathology
DOIs
StatePublished - 1 Jan 2015

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Ustilago
Ustilago zeae
dimorphism
Peptide Hydrolases
proteinases
pathogenesis
Vacuoles
Morphogenesis
mutants
Virulence
gene
morphogenesis
Fungi
virulence
Physiological Phenomena
Genes
vacuoles
Basidiomycota
genes
Mycelium

Cite this

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abstract = "{\circledC} 2015 BSPP and John Wiley & Sons Ltd. Vacuole proteases have important functions in different physiological processes in fungi. Taking this aspect into consideration, and as a continuation of our studies on the analysis of the proteolytic system of Ustilago maydis, a phytopathogenic member of the Basidiomycota, we have analysed the role of the pep4 gene encoding the vacuolar acid proteinase PrA in the pathogenesis and morphogenesis of the fungus. After confirmation of the location of the protease in the vacuole using fluorescent probes, we obtained deletion mutants of the gene in sexually compatible strains of U.maydis (FB1 and FB2), and analysed their phenotypes. It was observed that the yeast to mycelium dimorphic transition induced by a pH change in the medium, or the use of a fatty acid as sole carbon source, was severely reduced in Δpep4 mutants. In addition, the virulence of the mutants in maize seedlings was reduced, as revealed by the lower proportion of plants infected and the reduction in size of the tumours induced by the pathogen, when compared with wild-type strains. All of these phenotypic alterations were reversed by complementation of the mutant strains with the wild-type gene. These results provide evidence of the importance of the pep4 gene for the morphogenesis and virulence of U.maydis.",
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The pep4 gene encoding proteinase A is involved in dimorphism and pathogenesis of Ustilago maydis. / Soberanes-Gutiérrez, Cinthia V.; Juárez-Montiel, Margarita; Olguín-Rodríguez, Omar; Hernández-Rodríguez, César; Ruiz-Herrera, José; Villa-Tanaca, Lourdes.

In: Molecular Plant Pathology, 01.01.2015, p. 837-846.

Research output: Contribution to journalArticle

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T1 - The pep4 gene encoding proteinase A is involved in dimorphism and pathogenesis of Ustilago maydis

AU - Soberanes-Gutiérrez, Cinthia V.

AU - Juárez-Montiel, Margarita

AU - Olguín-Rodríguez, Omar

AU - Hernández-Rodríguez, César

AU - Ruiz-Herrera, José

AU - Villa-Tanaca, Lourdes

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AB - © 2015 BSPP and John Wiley & Sons Ltd. Vacuole proteases have important functions in different physiological processes in fungi. Taking this aspect into consideration, and as a continuation of our studies on the analysis of the proteolytic system of Ustilago maydis, a phytopathogenic member of the Basidiomycota, we have analysed the role of the pep4 gene encoding the vacuolar acid proteinase PrA in the pathogenesis and morphogenesis of the fungus. After confirmation of the location of the protease in the vacuole using fluorescent probes, we obtained deletion mutants of the gene in sexually compatible strains of U.maydis (FB1 and FB2), and analysed their phenotypes. It was observed that the yeast to mycelium dimorphic transition induced by a pH change in the medium, or the use of a fatty acid as sole carbon source, was severely reduced in Δpep4 mutants. In addition, the virulence of the mutants in maize seedlings was reduced, as revealed by the lower proportion of plants infected and the reduction in size of the tumours induced by the pathogen, when compared with wild-type strains. All of these phenotypic alterations were reversed by complementation of the mutant strains with the wild-type gene. These results provide evidence of the importance of the pep4 gene for the morphogenesis and virulence of U.maydis.

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