The outer membrane vesicles of aeromonas hydrophilaATCC®7966TM: A proteomic analysis and effect on host cells

Eric Daniel Avila-Calderón, Jorge Erick Otero-Olarra, Leopoldo Flores-Romo, Humberto Peralta, Ma Guadalupe Aguilera-Arreola, María Rosario Morales-García, Juana Calderón-Amador, Olin Medina-Chávez, Luis Donis-Maturano, María Del Socorro Ruiz-Palma, Araceli Contreras-Rodríguez

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Gram-negative bacteria release outer membrane vesicles (OMVs) into the extracellular environment. OMVs have been studied extensively in bacterial pathogens, however, information related with the composition of Aeromonas hydrophila OMVs is missing. In this study we analyzed the composition of purified OMVs from A. hydrophila ATCC® 7966TM by proteomics. Also we studied the effect of OMVs on human peripheral blood mononuclear cells (PBMCs). Vesicles were grown in agar plates and then purified through ultracentrifugation steps. Purified vesicles showed an average diameter of 90-170 nm. Moreover, 211 unique proteins were found in OMVs from A. hydrophila; some of them are well-known as virulence factors such as: Haemolysin Ahh1, RtxA toxin, extracellular lipase, HcpA protein, among others. OMVs from A. hydrophila ATCC® 7966TM induced lymphocyte activation and apoptosis in monocytes, as well as over-expression of pro-inflammatory cytokines. This work contributed to the knowledge of the composition of the vesicles of A. hydrophila ATCC® 7966TM and their interaction with the host cell.

Original languageEnglish
Article number2765
JournalFrontiers in Microbiology
Volume9
Issue numberNOV
DOIs
StatePublished - 16 Nov 2018

Keywords

  • Aeromonas
  • Bacterial vesicles
  • Gram-negative bacteria
  • Host cell immune response
  • Outer membrane vesicles
  • Pathogens

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