The effects of cold-pretreatment, auxins and carbon source on anther culture of rice

Gabriela Trejo-Tapia, Uriel Maldonado Amaya, Guadalupe Salcedo Morales, Antonia De Jesús Sánchez, Blanca Martínez Bonfil, Mario Rodríguez-Monroy, Antonio Jiménez-Aparicio

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51 Scopus citations

Abstract

The effects of a cold pretreatment, the concentration of different auxins (2,4-D, NAA and IAA) and the type of carbon source (maltose and sucrose) on the induction of callus from anthers of three parental lines and four rice F1 hybrids (Japonica x Indica, Indica x Japonica) were studied. The results indicated that a cold pretreatment was essential for the induction of callus from anthers of the parental lines and the F1 hybrids. These effects were genotype dependent. Auxins were essential for the induction of callus, and the type and concentration of auxins also influenced this process, as well as the type of carbon source. The greatest induction of callus was by the hybrid Morelos A92 x Koshihikari after a cold pretreatment of 8 days using 10.74 μM α-napthaleneacetic acid and 30 g 1-1 maltose.

Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalPlant Cell, Tissue and Organ Culture
Volume71
Issue number1
DOIs
StatePublished - Oct 2002

Keywords

  • 2,4-D
  • Callus induction
  • Haploids
  • IAA
  • Maltose
  • NAA
  • Oryza sativa L.
  • Plant growth regulators
  • Sucrose

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