TY - JOUR
T1 - The effects of cold-pretreatment, auxins and carbon source on anther culture of rice
AU - Trejo-Tapia, Gabriela
AU - Maldonado Amaya, Uriel
AU - Salcedo Morales, Guadalupe
AU - De Jesús Sánchez, Antonia
AU - Martínez Bonfil, Blanca
AU - Rodríguez-Monroy, Mario
AU - Jiménez-Aparicio, Antonio
N1 - Funding Information:
This work was supported by the Instituto Politec-nico Nacional (CGPI 998005/COFAA) and the Produce Morelos Foundation (grants 4I/A14/99 and 4I/A14/2000). Authors wish to thank IBQ. Neptaly Molina Reyes for the technical assistance.
PY - 2002/10
Y1 - 2002/10
N2 - The effects of a cold pretreatment, the concentration of different auxins (2,4-D, NAA and IAA) and the type of carbon source (maltose and sucrose) on the induction of callus from anthers of three parental lines and four rice F1 hybrids (Japonica x Indica, Indica x Japonica) were studied. The results indicated that a cold pretreatment was essential for the induction of callus from anthers of the parental lines and the F1 hybrids. These effects were genotype dependent. Auxins were essential for the induction of callus, and the type and concentration of auxins also influenced this process, as well as the type of carbon source. The greatest induction of callus was by the hybrid Morelos A92 x Koshihikari after a cold pretreatment of 8 days using 10.74 μM α-napthaleneacetic acid and 30 g 1-1 maltose.
AB - The effects of a cold pretreatment, the concentration of different auxins (2,4-D, NAA and IAA) and the type of carbon source (maltose and sucrose) on the induction of callus from anthers of three parental lines and four rice F1 hybrids (Japonica x Indica, Indica x Japonica) were studied. The results indicated that a cold pretreatment was essential for the induction of callus from anthers of the parental lines and the F1 hybrids. These effects were genotype dependent. Auxins were essential for the induction of callus, and the type and concentration of auxins also influenced this process, as well as the type of carbon source. The greatest induction of callus was by the hybrid Morelos A92 x Koshihikari after a cold pretreatment of 8 days using 10.74 μM α-napthaleneacetic acid and 30 g 1-1 maltose.
KW - 2,4-D
KW - Callus induction
KW - Haploids
KW - IAA
KW - Maltose
KW - NAA
KW - Oryza sativa L.
KW - Plant growth regulators
KW - Sucrose
UR - http://www.scopus.com/inward/record.url?scp=0036777723&partnerID=8YFLogxK
U2 - 10.1023/A:1016558025840
DO - 10.1023/A:1016558025840
M3 - Artículo
SN - 0167-6857
VL - 71
SP - 41
EP - 46
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
IS - 1
ER -