Targeting Several Biologically Reported Targets of Glioblastoma Multiforme by Assaying 2D and 3D Cultured Cells

Yudibeth Sixto-López; Emilie Marhuenda; Juan Benjamin García-Vazquez; Manuel Jonathan Fragoso-Vazquez; Martha Cecilia Rosales-Hernández; Oscar Zacarías-Lara; David Méndez-Luna; José Antonio Gómez-Vidal; David Cornu; Bakalara Norbert; José Correa-Basurto

doi:10.1007/s10571-021-01072-9

Targeting Several Biologically Reported Targets of Glioblastoma Multiforme by Assaying 2D and 3D Cultured Cells

Yudibeth Sixto-López, Emilie Marhuenda, Juan Benjamin García-Vazquez, Manuel Jonathan Fragoso-Vazquez, Martha Cecilia Rosales-Hernández, Oscar Zacarías-Lara, David Méndez-Luna, José Antonio Gómez-Vidal, David Cornu, Bakalara Norbert, José Correa-Basurto

Research output: Contribution to journal › Article › peer-review

Abstract

Glioblastoma multiforme (GBM) is account for 70% of all primary malignancies of the central nervous system. The median survival of human patients after treatment is around 15 months. There are several biological targets which have been reported that can be pursued using ligands with varied structures to treat this disease. In our group, we have developed several ligands that target a wide range of proteins involved in anticancer effects, such as histone deacetylase (HDACs), G protein-coupled estrogen receptor 1 (GPER), estrogen receptor-beta (ERβ) and NADPH oxidase (NOX), that were screened on bidimensional (2D) and tridimensional (3D) GBM stem cells like (GSC). Our results show that some HDAC inhibitors show antiproliferative properties at 21–32 µM. These results suggest that in this 3D culture, HDACs could be the most relevant targets that are modulated to induce the antiproliferative effects that require in the future further experimental studies.

Original language	English
Pages (from-to)	1909-1920
Number of pages	12
Journal	Cellular and Molecular Neurobiology
Volume	42
Issue number	6
DOIs	https://doi.org/10.1007/s10571-021-01072-9
State	Published - Aug 2022

Keywords

3D cell culture
ER
GPER
Glioblastoma multiforme
HDAC
NOX

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10571-021-01072-9

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Sixto-López, Y., Marhuenda, E., García-Vazquez, J. B., Fragoso-Vazquez, M. J., Rosales-Hernández, M. C., Zacarías-Lara, O., Méndez-Luna, D., Gómez-Vidal, J. A., Cornu, D., Norbert, B., & Correa-Basurto, J. (2022). Targeting Several Biologically Reported Targets of Glioblastoma Multiforme by Assaying 2D and 3D Cultured Cells. Cellular and Molecular Neurobiology, 42(6), 1909-1920. https://doi.org/10.1007/s10571-021-01072-9

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abstract = "Glioblastoma multiforme (GBM) is account for 70% of all primary malignancies of the central nervous system. The median survival of human patients after treatment is around 15 months. There are several biological targets which have been reported that can be pursued using ligands with varied structures to treat this disease. In our group, we have developed several ligands that target a wide range of proteins involved in anticancer effects, such as histone deacetylase (HDACs), G protein-coupled estrogen receptor 1 (GPER), estrogen receptor-beta (ERβ) and NADPH oxidase (NOX), that were screened on bidimensional (2D) and tridimensional (3D) GBM stem cells like (GSC). Our results show that some HDAC inhibitors show antiproliferative properties at 21–32 µM. These results suggest that in this 3D culture, HDACs could be the most relevant targets that are modulated to induce the antiproliferative effects that require in the future further experimental studies.",

keywords = "3D cell culture, ER, GPER, Glioblastoma multiforme, HDAC, NOX",

author = "Yudibeth Sixto-L{\'o}pez and Emilie Marhuenda and Garc{\'i}a-Vazquez, {Juan Benjamin} and Fragoso-Vazquez, {Manuel Jonathan} and Rosales-Hern{\'a}ndez, {Martha Cecilia} and Oscar Zacar{\'i}as-Lara and David M{\'e}ndez-Luna and G{\'o}mez-Vidal, {Jos{\'e} Antonio} and David Cornu and Bakalara Norbert and Jos{\'e} Correa-Basurto",

note = "Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.",

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Sixto-López, Y, Marhuenda, E, García-Vazquez, JB , Fragoso-Vazquez, MJ , Rosales-Hernández, MC, Zacarías-Lara, O, Méndez-Luna, D, Gómez-Vidal, JA, Cornu, D, Norbert, B & Correa-Basurto, J 2022, 'Targeting Several Biologically Reported Targets of Glioblastoma Multiforme by Assaying 2D and 3D Cultured Cells', Cellular and Molecular Neurobiology, vol. 42, no. 6, pp. 1909-1920. https://doi.org/10.1007/s10571-021-01072-9

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AU - Sixto-López, Yudibeth

AU - Marhuenda, Emilie

AU - García-Vazquez, Juan Benjamin

AU - Fragoso-Vazquez, Manuel Jonathan

AU - Rosales-Hernández, Martha Cecilia

AU - Zacarías-Lara, Oscar

AU - Méndez-Luna, David

AU - Gómez-Vidal, José Antonio

AU - Cornu, David

AU - Norbert, Bakalara

AU - Correa-Basurto, José

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N2 - Glioblastoma multiforme (GBM) is account for 70% of all primary malignancies of the central nervous system. The median survival of human patients after treatment is around 15 months. There are several biological targets which have been reported that can be pursued using ligands with varied structures to treat this disease. In our group, we have developed several ligands that target a wide range of proteins involved in anticancer effects, such as histone deacetylase (HDACs), G protein-coupled estrogen receptor 1 (GPER), estrogen receptor-beta (ERβ) and NADPH oxidase (NOX), that were screened on bidimensional (2D) and tridimensional (3D) GBM stem cells like (GSC). Our results show that some HDAC inhibitors show antiproliferative properties at 21–32 µM. These results suggest that in this 3D culture, HDACs could be the most relevant targets that are modulated to induce the antiproliferative effects that require in the future further experimental studies.

AB - Glioblastoma multiforme (GBM) is account for 70% of all primary malignancies of the central nervous system. The median survival of human patients after treatment is around 15 months. There are several biological targets which have been reported that can be pursued using ligands with varied structures to treat this disease. In our group, we have developed several ligands that target a wide range of proteins involved in anticancer effects, such as histone deacetylase (HDACs), G protein-coupled estrogen receptor 1 (GPER), estrogen receptor-beta (ERβ) and NADPH oxidase (NOX), that were screened on bidimensional (2D) and tridimensional (3D) GBM stem cells like (GSC). Our results show that some HDAC inhibitors show antiproliferative properties at 21–32 µM. These results suggest that in this 3D culture, HDACs could be the most relevant targets that are modulated to induce the antiproliferative effects that require in the future further experimental studies.

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JO - Cellular and Molecular Neurobiology

JF - Cellular and Molecular Neurobiology

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ER -

Targeting Several Biologically Reported Targets of Glioblastoma Multiforme by Assaying 2D and 3D Cultured Cells

Abstract

Keywords

UN SDGs

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