TY - JOUR
T1 - Tabebuia rosea (Bertol.) DC. ethanol extract attenuates body weight gain by activation of molecular mediators associated with browning
AU - Pagaza-Straffon, Elsa Cecilia
AU - Mezo-González, Carla Elena
AU - Chavaro-Pérez, David Armando
AU - Cornejo-Garrido, Jorge
AU - Marchat, Laurence A.
AU - Benítez-Cardoza, Claudia G.
AU - Anaya-Reyes, Maricruz
AU - Ordaz-Pichardo, Cynthia
N1 - Funding Information:
Pagaza-Straffon EC is a student in the Program of Doctorate in Sciences in Biotechnology at the National Polytechnic Institute who received a Scholarship for Institutional Stimulus for Researchers Training (BEIFI) and the CONACYT-M?xico Scholarship. Mezo-Gonz?lez CE and Chavaro-P?rez DA were students in the Program of Master of Science in Molecular Biomedicine at the National Polytechnic Institute who received the CONACYT-M?xico Scholarship. Cornejo-Garrido J, Marchat L, Ben?tez Cardoza CG and Ordaz-Pichardo C received fellowships from Commission of Operation and Promotion of Academic Activities (COFAA) and Encouragement of Researchers' Performance (EDI). The authors are grateful for financial support Projects SIP-IPN M?xico 20200739 and 20200732 and Productos Medix S.A. de C.V. project 003572. Our thanks also go to Edith L?pez-Villafranco MSc. for the taxonomic identification of T. rosea, to the company REDSA SA de CV, for their support in grinding the bark of T. rosea, to Ricardo Gaxiola-Centeno Vet. for the care of the laboratory animals, and Yarely Marlene Salinas- Vera MSc for its support in the technique of qRT-PCR. We confirm that any aspect of the work covered in this manuscript that has involved either experimental animal has been conducted with the ethical approval of all relevant bodies and that such approvals are acknowledged within the manuscript. The experiments were carried out in the Laboratory Animal Maintenance Room (SMAL, for its acronym in Spanish) of the ENMH of IPN according to Official Mexican Standards NOM-062-ZOO-1999 and NOM-033-Z00-1995, and under protocols approved by the Bioethics Committee of the ENMH with the number ENMH-CB 146-2015 and ENMH-2017, complying with International Regulations and Policies regarding the use, care, and sacrifice of laboratory animals.
Funding Information:
Pagaza-Straffon EC is a student in the Program of Doctorate in Sciences in Biotechnology at the National Polytechnic Institute who received a Scholarship for Institutional Stimulus for Researchers Training (BEIFI) and the CONACYT-México Scholarship. Mezo-González CE and Chavaro-Pérez DA were students in the Program of Master of Science in Molecular Biomedicine at the National Polytechnic Institute who received the CONACYT-México Scholarship. Cornejo-Garrido J, Marchat L, Benítez Cardoza CG and Ordaz-Pichardo C received fellowships from Commission of Operation and Promotion of Academic Activities (COFAA) and Encouragement of Researchers' Performance (EDI). The authors are grateful for financial support Projects SIP-IPN México 20200739 and 20200732 and Productos Medix S.A. de C.V., project 003572. Our thanks also go to Edith López-Villafranco MSc. for the taxonomic identification of T. rosea, to the company REDSA SA de CV, for their support in grinding the bark of T. rosea, to Ricardo Gaxiola-Centeno Vet., for the care of the laboratory animals, and Yarely Marlene Salinas- Vera MSc for its support in the technique of qRT-PCR.
Publisher Copyright:
© 2021
PY - 2021/11
Y1 - 2021/11
N2 - Tabebuia rosea has been reported to have anti-cancer, antioxidant, and hypoglycemic activities, and other species of the same genus have anti-obesity effect. Ethanol extract of T. rosea (TrEtOH) produced a significant decrease in body weight (12.1%), and a diminution in lipid profile markers in high fat diet-induced obese C57BL/6 mice. A reduction in the number of hypertrophic adipocytes and a decrease of triglycerides was observed in adipose tissue, added to a reduction in size and fat in hepatocytes. The administration of TrEtOH leads to the mRNA overexpression of UCP1, TBX1, and GLUT4, suggesting that the extract promotes adipocyte browning as a possible mechanism of action. A glycosylated coumarin, lapachol and quercetin were quantified in TrEtOH extract. Interestingly TrEtOH extract did not show mutagenicity, genotoxicity, or toxic effects after acute or 28 days repeated administration. TrEtOH extract could be used for the development of new drugs against obesity and its comorbidities.
AB - Tabebuia rosea has been reported to have anti-cancer, antioxidant, and hypoglycemic activities, and other species of the same genus have anti-obesity effect. Ethanol extract of T. rosea (TrEtOH) produced a significant decrease in body weight (12.1%), and a diminution in lipid profile markers in high fat diet-induced obese C57BL/6 mice. A reduction in the number of hypertrophic adipocytes and a decrease of triglycerides was observed in adipose tissue, added to a reduction in size and fat in hepatocytes. The administration of TrEtOH leads to the mRNA overexpression of UCP1, TBX1, and GLUT4, suggesting that the extract promotes adipocyte browning as a possible mechanism of action. A glycosylated coumarin, lapachol and quercetin were quantified in TrEtOH extract. Interestingly TrEtOH extract did not show mutagenicity, genotoxicity, or toxic effects after acute or 28 days repeated administration. TrEtOH extract could be used for the development of new drugs against obesity and its comorbidities.
KW - Browning
KW - Obesity
KW - Polyphenols
KW - Tabebuia rosea
KW - Toxicity
UR - http://www.scopus.com/inward/record.url?scp=85114696407&partnerID=8YFLogxK
U2 - 10.1016/j.jff.2021.104740
DO - 10.1016/j.jff.2021.104740
M3 - Artículo
AN - SCOPUS:85114696407
VL - 86
JO - Journal of Functional Foods
JF - Journal of Functional Foods
SN - 1756-4646
M1 - 104740
ER -
