Synthesis and evaluation of the cardiovascular effects of two, membrane impermeant, macromolecular complexes of dextran-testosterone

Lauro Figueroa-Valverde, Hector Luna, Carlos Castillo-Henkel, Olga Muñoz-Garcia, Tomas Morato-Cartagena, Guillermo Ceballos-Reyes

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The incidence of cardiovascular disease is greater in men than in premenopausal women. Testosterone has been considered a significant risk factor for cardiovascular disease, but testosterone's mechanism of action and its cellular site of action are still not clear. However, it is likely that non-genomic extracellular effects of the hormone are involved. With the aim of providing further information about this phenomenon, two membrane impermeant, macromolecular complexes of testosterone were synthesized and their cardiovascular effects were evaluated. We covalently bound testosterone (through carbon 3 or C-17 functional groups) to dextran (2 MDa) and evaluated its effects on isolated and perfused rat hearts (Langerdorff model). Our results showed that the macromolecular complexes increased vascular resistance similarly to free testosterone and blocked adenosine-induced vasodilatation. These effects were exerted rapidly and possibly through a non-genomic mechanism. Blockade of C-3 or C-17 functional groups by binding to macromolecular dextran induced no qualitative and/or quantitative changes in testosterone-induced effects. © 2002 Elsevier Science Inc. All rights reserved.
Original languageAmerican English
Pages (from-to)611-619
Number of pages549
JournalSteroids
DOIs
StatePublished - 13 May 2002

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Macromolecular Substances
Dextrans
Testosterone
Membranes
Functional groups
Cardiovascular Diseases
Vasodilation
Vascular Resistance
Adenosine
Rats
Carbon
Hormones
Incidence

Cite this

Figueroa-Valverde, Lauro ; Luna, Hector ; Castillo-Henkel, Carlos ; Muñoz-Garcia, Olga ; Morato-Cartagena, Tomas ; Ceballos-Reyes, Guillermo. / Synthesis and evaluation of the cardiovascular effects of two, membrane impermeant, macromolecular complexes of dextran-testosterone. In: Steroids. 2002 ; pp. 611-619.
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Synthesis and evaluation of the cardiovascular effects of two, membrane impermeant, macromolecular complexes of dextran-testosterone. / Figueroa-Valverde, Lauro; Luna, Hector; Castillo-Henkel, Carlos; Muñoz-Garcia, Olga; Morato-Cartagena, Tomas; Ceballos-Reyes, Guillermo.

In: Steroids, 13.05.2002, p. 611-619.

Research output: Contribution to journalArticle

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