Produção sustentável para obtenção de glucosamina a partir de exoesqueletos de caranguejo

Translated title of the contribution: Sustainable production with obtaining glucosamine from crab exoskeletons

Jorge Montiel-Montoya, Maribel Valdez-Morales, Cesar Reyes, Hebert Jair Barrales-Cureño

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Two chemical treatments, five enzymatic (pectinase, lipase, hemicellulase, hemicellulose-cellulase or lipase-pectinase) and one microbiological (Bacillus subtilis) treatment were evaluated to obtain glucosamine hydrochloride (Gluc-HCl) from the chitin obtained from crab (Callinectes bellicosus) exoskeletons. Chemical treatments were referred as Method A (HCl hydrolysis during 75 min at 90°C) and Method B (HCl hydrolysis during 20 min and 14 h of rest). Glucosamine and, in some cases, N-acetyl-D-glucosamine were identified and quantified by HPLC. Treatments with the greater concentrations of Gluc-HCl in descending order were: Lipase (94.4 mg/g), microbiological (45.7 mg/g), lipase-pectinase (22.9 mg/g), hemicellulase-cellulase (20.9 mg/g), hemicellulase (15.3 mg/g), pectinase (10.7 mg/g), Chemical A (7.3mg/g) and Chemical B (7.3mg/g). In terms of yield, the best treatments in descending order were: Pectinase (94%), microbiological (94%), hemicellulase (92%), lipase (91%), Chemical B (88%), lipase-pectinase (88%), hemicellulase-cellulase (86%) and Chemical A (28.5%). The two most profound treatments were lipase and microbiological, so they are proposed as part of a viable method to produce Gluc-HCl from crab exoskeletons; they are ecofriendly procedures and could add value to the crab´s productive chain.

Translated title of the contributionSustainable production with obtaining glucosamine from crab exoskeletons
Original languagePortuguese
Article numbere20190021
JournalCiencia Rural
Issue number9
StatePublished - 1 Jan 2019


  • Callinectes bellicosus
  • Extraction methods
  • Glucosamine hydrochloride
  • HPLC analysis


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