Survival and immune-related gene expression in Litopenaeus vannamei co-infected with WSSV and Vibrio parahaemolyticus

Arturo Rubio-Castro, Antonio Luna-Gonz�lez, P�ndaro �lvarez-Ruiz, Ruth Escamilla-Montes, Jes�s A. Fierro-Coronado, Patricia L�pez-Le�n, Ma del Carmen Flores-Miranda, Genaro Diarte-Plata

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

� 2016 Elsevier B.V. In the present study, survival and immune-related gene expression were investigated in Litopenaeus vannamei co-infected with white spot syndrome virus (WSSV) and Vibrio parahaemolyticus by ingestion and immersion, respectively. The expression of five immune-related genes (translationally controlled tumor protein [TCTP], toll receptor [LvToll] , penaeidin4, crustin, and lysozyme) in hemocytes of experimental shrimp was measured by real-time quantitative PCR. Shrimp infected first with V. parahaemolyticus and then with WSSV showed a mortality of 46.66���0.88%. Shrimp infected first with WSSV and then with V. parahaemolyticus showed a mortality of 45.00���1.53%. Shrimp infected with WSSV and V. parahaemolyticus at the same time showed a mortality of 55.00���2.08%. Gene expression of LvToll, crustin, and lysozyme was upregulated whereas gene expression of TCTP and penaeidin4 was downregulated. Under the present experimental conditions and infection routes, V. parahaemolyticus was the main responsible for the mortality of cultured white shrimp. Regarding immune response, significant changes were found in gene expression in hemocytes of L. vannamei during the time-course of a successful immune response to pathogens V. parahaemolyticus and WSSV. Statement of relevance Bacterial and viral co-infection in white shrimp culture.
Original languageAmerican English
Pages (from-to)692-698
Number of pages7
JournalAquaculture
DOIs
StatePublished - 1 Nov 2016
Externally publishedYes

Fingerprint

White spot syndrome virus
Vibrio parahaemolyticus
Litopenaeus vannamei
gene expression
virus
shrimp
crustin
mortality
immune response
tumor
hemocytes
lysozyme
shrimp culture
protein
neoplasms
mixed infection
pathogen
quantitative polymerase chain reaction
proteins
ingestion

Cite this

Rubio-Castro, A., Luna-Gonz�lez, A., �lvarez-Ruiz, P., Escamilla-Montes, R., Fierro-Coronado, J. A., L�pez-Le�n, P., ... Diarte-Plata, G. (2016). Survival and immune-related gene expression in Litopenaeus vannamei co-infected with WSSV and Vibrio parahaemolyticus. Aquaculture, 692-698. https://doi.org/10.1016/j.aquaculture.2016.08.024
Rubio-Castro, Arturo ; Luna-Gonz�lez, Antonio ; �lvarez-Ruiz, P�ndaro ; Escamilla-Montes, Ruth ; Fierro-Coronado, Jes�s A. ; L�pez-Le�n, Patricia ; Flores-Miranda, Ma del Carmen ; Diarte-Plata, Genaro. / Survival and immune-related gene expression in Litopenaeus vannamei co-infected with WSSV and Vibrio parahaemolyticus. In: Aquaculture. 2016 ; pp. 692-698.
@article{21e5160eae8e47f9afee3f4f3977b70f,
title = "Survival and immune-related gene expression in Litopenaeus vannamei co-infected with WSSV and Vibrio parahaemolyticus",
abstract = "{\"i}¿½ 2016 Elsevier B.V. In the present study, survival and immune-related gene expression were investigated in Litopenaeus vannamei co-infected with white spot syndrome virus (WSSV) and Vibrio parahaemolyticus by ingestion and immersion, respectively. The expression of five immune-related genes (translationally controlled tumor protein [TCTP], toll receptor [LvToll] , penaeidin4, crustin, and lysozyme) in hemocytes of experimental shrimp was measured by real-time quantitative PCR. Shrimp infected first with V. parahaemolyticus and then with WSSV showed a mortality of 46.66{\"i}¿½{\"i}¿½{\"i}¿½0.88{\%}. Shrimp infected first with WSSV and then with V. parahaemolyticus showed a mortality of 45.00{\"i}¿½{\"i}¿½{\"i}¿½1.53{\%}. Shrimp infected with WSSV and V. parahaemolyticus at the same time showed a mortality of 55.00{\"i}¿½{\"i}¿½{\"i}¿½2.08{\%}. Gene expression of LvToll, crustin, and lysozyme was upregulated whereas gene expression of TCTP and penaeidin4 was downregulated. Under the present experimental conditions and infection routes, V. parahaemolyticus was the main responsible for the mortality of cultured white shrimp. Regarding immune response, significant changes were found in gene expression in hemocytes of L. vannamei during the time-course of a successful immune response to pathogens V. parahaemolyticus and WSSV. Statement of relevance Bacterial and viral co-infection in white shrimp culture.",
author = "Arturo Rubio-Castro and Antonio Luna-Gonz{\"i}¿½lez and P{\"i}¿½ndaro {\"i}¿½lvarez-Ruiz and Ruth Escamilla-Montes and Fierro-Coronado, {Jes{\"i}¿½s A.} and Patricia L{\"i}¿½pez-Le{\"i}¿½n and Flores-Miranda, {Ma del Carmen} and Genaro Diarte-Plata",
year = "2016",
month = "11",
day = "1",
doi = "10.1016/j.aquaculture.2016.08.024",
language = "American English",
pages = "692--698",
journal = "Aquaculture",
issn = "0044-8486",
publisher = "Elsevier",

}

Rubio-Castro, A, Luna-Gonz�lez, A, �lvarez-Ruiz, P, Escamilla-Montes, R, Fierro-Coronado, JA, L�pez-Le�n, P, Flores-Miranda, MDC & Diarte-Plata, G 2016, 'Survival and immune-related gene expression in Litopenaeus vannamei co-infected with WSSV and Vibrio parahaemolyticus', Aquaculture, pp. 692-698. https://doi.org/10.1016/j.aquaculture.2016.08.024

Survival and immune-related gene expression in Litopenaeus vannamei co-infected with WSSV and Vibrio parahaemolyticus. / Rubio-Castro, Arturo; Luna-Gonz�lez, Antonio; �lvarez-Ruiz, P�ndaro; Escamilla-Montes, Ruth; Fierro-Coronado, Jes�s A.; L�pez-Le�n, Patricia; Flores-Miranda, Ma del Carmen; Diarte-Plata, Genaro.

In: Aquaculture, 01.11.2016, p. 692-698.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Survival and immune-related gene expression in Litopenaeus vannamei co-infected with WSSV and Vibrio parahaemolyticus

AU - Rubio-Castro, Arturo

AU - Luna-Gonz�lez, Antonio

AU - �lvarez-Ruiz, P�ndaro

AU - Escamilla-Montes, Ruth

AU - Fierro-Coronado, Jes�s A.

AU - L�pez-Le�n, Patricia

AU - Flores-Miranda, Ma del Carmen

AU - Diarte-Plata, Genaro

PY - 2016/11/1

Y1 - 2016/11/1

N2 - � 2016 Elsevier B.V. In the present study, survival and immune-related gene expression were investigated in Litopenaeus vannamei co-infected with white spot syndrome virus (WSSV) and Vibrio parahaemolyticus by ingestion and immersion, respectively. The expression of five immune-related genes (translationally controlled tumor protein [TCTP], toll receptor [LvToll] , penaeidin4, crustin, and lysozyme) in hemocytes of experimental shrimp was measured by real-time quantitative PCR. Shrimp infected first with V. parahaemolyticus and then with WSSV showed a mortality of 46.66���0.88%. Shrimp infected first with WSSV and then with V. parahaemolyticus showed a mortality of 45.00���1.53%. Shrimp infected with WSSV and V. parahaemolyticus at the same time showed a mortality of 55.00���2.08%. Gene expression of LvToll, crustin, and lysozyme was upregulated whereas gene expression of TCTP and penaeidin4 was downregulated. Under the present experimental conditions and infection routes, V. parahaemolyticus was the main responsible for the mortality of cultured white shrimp. Regarding immune response, significant changes were found in gene expression in hemocytes of L. vannamei during the time-course of a successful immune response to pathogens V. parahaemolyticus and WSSV. Statement of relevance Bacterial and viral co-infection in white shrimp culture.

AB - � 2016 Elsevier B.V. In the present study, survival and immune-related gene expression were investigated in Litopenaeus vannamei co-infected with white spot syndrome virus (WSSV) and Vibrio parahaemolyticus by ingestion and immersion, respectively. The expression of five immune-related genes (translationally controlled tumor protein [TCTP], toll receptor [LvToll] , penaeidin4, crustin, and lysozyme) in hemocytes of experimental shrimp was measured by real-time quantitative PCR. Shrimp infected first with V. parahaemolyticus and then with WSSV showed a mortality of 46.66���0.88%. Shrimp infected first with WSSV and then with V. parahaemolyticus showed a mortality of 45.00���1.53%. Shrimp infected with WSSV and V. parahaemolyticus at the same time showed a mortality of 55.00���2.08%. Gene expression of LvToll, crustin, and lysozyme was upregulated whereas gene expression of TCTP and penaeidin4 was downregulated. Under the present experimental conditions and infection routes, V. parahaemolyticus was the main responsible for the mortality of cultured white shrimp. Regarding immune response, significant changes were found in gene expression in hemocytes of L. vannamei during the time-course of a successful immune response to pathogens V. parahaemolyticus and WSSV. Statement of relevance Bacterial and viral co-infection in white shrimp culture.

U2 - 10.1016/j.aquaculture.2016.08.024

DO - 10.1016/j.aquaculture.2016.08.024

M3 - Article

SP - 692

EP - 698

JO - Aquaculture

JF - Aquaculture

SN - 0044-8486

ER -

Rubio-Castro A, Luna-Gonz�lez A, �lvarez-Ruiz P, Escamilla-Montes R, Fierro-Coronado JA, L�pez-Le�n P et al. Survival and immune-related gene expression in Litopenaeus vannamei co-infected with WSSV and Vibrio parahaemolyticus. Aquaculture. 2016 Nov 1;692-698. https://doi.org/10.1016/j.aquaculture.2016.08.024