Surveillance for flaviviruses near the Mexico-U.S. Border: Co-circulation of dengue virus serotypes 1,2, and 3 and West Nile Virus in Tamaulipas, Northern Mexico, 2014-2016

S. Viridiana Laredo-Tiscareño, Javier A. Garza-Hernandez, Ma Isabel Salazar, Erick J. De Luna-Santillana, Chandra S. Tangudu, Rosa C. Cetina-Trejo, Gloria L. Doria-Cobos, Santos Daniel Carmona-Aguirre, Julian E. Garcia-Rejon, Carlos Machain-Williams, Bradley J. Blitvich, Mario Alberto Rodríguez Pérez

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

A clinical, serological, and molecular investigation was performed to determine the presence of dengue virus (DENV) and other flaviviruses among residents of the city of Reynosa, Tamaulipas, on the Mexico-U.S. border in 2014-2016. The sample population consisted of 2,355 patients with suspected dengue, in addition to 346 asymptomatic individuals recruited during a household-based epidemiological investigation designed to identify flavivirus seroconversions. Sera were collected from patients with suspected dengue in the acute phase of illness and from asymptomatic individuals at enrollment and every 5-7 months for 19 months. Sera from suspected dengue patients were tested for DENV antigen by enzyme-linked immunosorbent assay (ELISA), and select antigen-positive sera were further tested using a serotype-specific, quantitative reverse transcription-polymerase chain reaction. Sera from the household cohort were tested for flavivirus-reactive antibodies by immunoglobulin (Ig) M and IgG ELISAs using DENV antigen. A total of 418 (17.7%) patients with suspected dengue had laboratory-confirmed DENV infections, including 82 patients who were positive for DENV RNA. The most frequently detected serotype was DENV-1 (61 patients), followed by DENV-2 (16 patients) and DENV-3 (five patients). Atotal of 217 (62.7%) asymptomatic individuals had flavivirus-reactive antibodies at enrollment, and nine flavivirus-näve individuals seroconverted. Sera from a subset of dengue patients and household participants, including all those who seroconverted, were further tested by plaque reduction neutralization test, resulting in the detection of antibodies to DENV-1, DENV-2, and West Nile virus. In summary, we provide evidence for the co-circulation of multiple flaviviruses in Reynosa, Tamaulipas, on the Mexico-U.S. border.

Original languageEnglish
Pages (from-to)1308-1317
Number of pages10
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume99
Issue number5
DOIs
StatePublished - 2018

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