1H-NMR metabolomics profiling of recombinant tobacco plants holding a promoter of a sesquiterpene cyclase

Elvia Becerra-Martínez, Yesenia Pacheco-Hernández, Edmundo Lozoya-Gloria, Martha G. Betancourt-Jiménez, Diego Hidalgo-Martínez, Luis G. Zepeda-Vallejo, Nemesio Villa-Ruano

Research output: Contribution to journalArticle

Abstract

© 2020 John Wiley & Sons, Ltd. Introduction: Nicotiana tabacum is a plant model intensively used in the bio-engineering pharmaceutical industry as a platform to produce drugs and therapeutic agents. Currently, no information regarding the non-targeted metabolome of transgenic tobacco containing recombinant regulatory sequences is available. Objective: To compare the proton nuclear magnetic resonance (1H-NMR) metabolomics profiling of a recombinant Nicotiana tabacum strain containing a promoter of a sesquiterpene cyclase from Capsicum annuum driving GUS expression, versus wild-type samples. Methodology. The non-targeted 1H-NMR metabolome was obtained and processed by principal component analysis (PCA) and orthogonal projection to latent structures discriminant analysis (OPLS-DA). The differential metabolites were quantified by quantitative NMR. Results: PCA and OPLS-DA revealed 37 metabolites including 16 discriminant compounds for transgenic samples. Ethanol (0.4 mg g-1), the main differential compound, was exclusively detected in transgenic tobacco; however, high levels of formate (0.28 mg g-1) and acetate (0.3 mg g-1) were simultaneously observed in the same group of samples. Cembratriene-4,6-diol, an antitumour and neuroprotective compound, and capsidiol, a known phytoalexin, increased by about 30% in transgenic samples. In addition, the endogenous levels of the antioxidant caffeoylquinic acid isomers increased by 50% in comparison to those of wild-type tobaccos. Conclusion: Our results support the occurrence of metabolic differences between wild type and transgenic tobacco containing a promoter of a Capsicum sesquiterpene cyclase gene. Interestingly, the recombinant transgenic strain studied accumulated high amounts of added value compounds with biological activity.
Original languageAmerican English
Pages (from-to)480-487
Number of pages8
JournalPhytochemical Analysis
DOIs
StatePublished - 1 Jul 2020

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    Becerra-Martínez, E., Pacheco-Hernández, Y., Lozoya-Gloria, E., Betancourt-Jiménez, M. G., Hidalgo-Martínez, D., Zepeda-Vallejo, L. G., & Villa-Ruano, N. (2020). 1H-NMR metabolomics profiling of recombinant tobacco plants holding a promoter of a sesquiterpene cyclase. Phytochemical Analysis, 480-487. https://doi.org/10.1002/pca.2911