TY - JOUR
T1 - 1H NMR-based metabolomic profiling for identification of metabolites in Capsicum annuum cv. mirasol infected by beet mild curly top virus (BMCTV)
AU - Villa-Ruano, Nemesio
AU - Velásquez-Valle, Rodolfo
AU - Zepeda-Vallejo, L. Gerardo
AU - Pérez-Hernández, Nury
AU - Velázquez-Ponce, Manuel
AU - Arcos-Adame, Victor M.
AU - Becerra-Martínez, Elvia
N1 - Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/4
Y1 - 2018/4
N2 - Beet mild curly top virus (BMCTV) is associated with an outbreak of curly top in chili pepper, tomato and other Solanaceae species, which can cause severe crop losses. The aim of this work was to obtain the 1H NMR metabolomic profiling of both healthy chili peppers (cv. mirasol) and infected chili peppers with BMCTV in order to find chemical markers associated to the infection process. Significant differences were found between the two groups, according to principal component analysis and orthogonal projections to latent structure discriminant analysis. Compared to the asymptomatic peppers, the symptomatic fruits had higher relative abundance of fructose, isoleucine, histidine, phenylalanine and tryptophan. Contrarily, the asymptomatic samples showed greater amounts of malonate and isobutyrate. These results suggest that in diseased chili peppers there are metabolic changes related to the viral acquisition of energy for replication and capsid assembly. This is the first study describing the chemical profiling of a polar extract obtained from Capsicum annuum infected by BMCTV under open field conditions.
AB - Beet mild curly top virus (BMCTV) is associated with an outbreak of curly top in chili pepper, tomato and other Solanaceae species, which can cause severe crop losses. The aim of this work was to obtain the 1H NMR metabolomic profiling of both healthy chili peppers (cv. mirasol) and infected chili peppers with BMCTV in order to find chemical markers associated to the infection process. Significant differences were found between the two groups, according to principal component analysis and orthogonal projections to latent structure discriminant analysis. Compared to the asymptomatic peppers, the symptomatic fruits had higher relative abundance of fructose, isoleucine, histidine, phenylalanine and tryptophan. Contrarily, the asymptomatic samples showed greater amounts of malonate and isobutyrate. These results suggest that in diseased chili peppers there are metabolic changes related to the viral acquisition of energy for replication and capsid assembly. This is the first study describing the chemical profiling of a polar extract obtained from Capsicum annuum infected by BMCTV under open field conditions.
KW - Beet mild curly top virus
KW - Capsicum annuum
KW - H NMR
KW - Metabolomic profiling
KW - Principal component analysis
UR - http://www.scopus.com/inward/record.url?scp=85041451814&partnerID=8YFLogxK
U2 - 10.1016/j.foodres.2018.01.065
DO - 10.1016/j.foodres.2018.01.065
M3 - Artículo
C2 - 29579998
SN - 0963-9969
VL - 106
SP - 870
EP - 877
JO - Food Research International
JF - Food Research International
ER -