1H NMR-based metabolomic profiling for identification of metabolites in Capsicum annuum cv. mirasol infected by beet mild curly top virus (BMCTV)

Nemesio Villa-Ruano, Rodolfo Velásquez-Valle, L. Gerardo Zepeda-Vallejo, Nury Pérez-Hernández, Manuel Velázquez-Ponce, Victor M. Arcos-Adame, Elvia Becerra-Martínez

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Beet mild curly top virus (BMCTV) is associated with an outbreak of curly top in chili pepper, tomato and other Solanaceae species, which can cause severe crop losses. The aim of this work was to obtain the 1H NMR metabolomic profiling of both healthy chili peppers (cv. mirasol) and infected chili peppers with BMCTV in order to find chemical markers associated to the infection process. Significant differences were found between the two groups, according to principal component analysis and orthogonal projections to latent structure discriminant analysis. Compared to the asymptomatic peppers, the symptomatic fruits had higher relative abundance of fructose, isoleucine, histidine, phenylalanine and tryptophan. Contrarily, the asymptomatic samples showed greater amounts of malonate and isobutyrate. These results suggest that in diseased chili peppers there are metabolic changes related to the viral acquisition of energy for replication and capsid assembly. This is the first study describing the chemical profiling of a polar extract obtained from Capsicum annuum infected by BMCTV under open field conditions.

Original languageEnglish
Pages (from-to)870-877
Number of pages8
JournalFood Research International
Volume106
DOIs
StatePublished - Apr 2018

Keywords

  • Beet mild curly top virus
  • Capsicum annuum
  • H NMR
  • Metabolomic profiling
  • Principal component analysis

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