1H NMR-based metabolomic profiling for identification of metabolites in Capsicum annuum cv. mirasol infected by beet mild curly top virus (BMCTV)

Nemesio Villa-Ruano, Rodolfo Velásquez-Valle, L. Gerardo Zepeda-Vallejo, Nury Pérez-Hernández, Manuel Velázquez-Ponce, Victor M. Arcos-Adame, Elvia Becerra-Martínez

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Abstract

© 2018 Elsevier Ltd Beet mild curly top virus (BMCTV) is associated with an outbreak of curly top in chili pepper, tomato and other Solanaceae species, which can cause severe crop losses. The aim of this work was to obtain the 1H NMR metabolomic profiling of both healthy chili peppers (cv. mirasol) and infected chili peppers with BMCTV in order to find chemical markers associated to the infection process. Significant differences were found between the two groups, according to principal component analysis and orthogonal projections to latent structure discriminant analysis. Compared to the asymptomatic peppers, the symptomatic fruits had higher relative abundance of fructose, isoleucine, histidine, phenylalanine and tryptophan. Contrarily, the asymptomatic samples showed greater amounts of malonate and isobutyrate. These results suggest that in diseased chili peppers there are metabolic changes related to the viral acquisition of energy for replication and capsid assembly. This is the first study describing the chemical profiling of a polar extract obtained from Capsicum annuum infected by BMCTV under open field conditions.
Original languageAmerican English
Pages (from-to)870-877
Number of pages782
JournalFood Research International
DOIs
StatePublished - 1 Apr 2018

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Beet mild curly top virus
Capsicum
Beta vulgaris
Metabolomics
hot peppers
metabolomics
Capsicum annuum
metabolites
Viruses
capsid
Isobutyrates
crop losses
Solanaceae
sweet peppers
isoleucine
histidine
phenylalanine
discriminant analysis
tryptophan
Isoleucine

Cite this

@article{38c66223148641938239e78c73824e00,
title = "1H NMR-based metabolomic profiling for identification of metabolites in Capsicum annuum cv. mirasol infected by beet mild curly top virus (BMCTV)",
abstract = "{\circledC} 2018 Elsevier Ltd Beet mild curly top virus (BMCTV) is associated with an outbreak of curly top in chili pepper, tomato and other Solanaceae species, which can cause severe crop losses. The aim of this work was to obtain the 1H NMR metabolomic profiling of both healthy chili peppers (cv. mirasol) and infected chili peppers with BMCTV in order to find chemical markers associated to the infection process. Significant differences were found between the two groups, according to principal component analysis and orthogonal projections to latent structure discriminant analysis. Compared to the asymptomatic peppers, the symptomatic fruits had higher relative abundance of fructose, isoleucine, histidine, phenylalanine and tryptophan. Contrarily, the asymptomatic samples showed greater amounts of malonate and isobutyrate. These results suggest that in diseased chili peppers there are metabolic changes related to the viral acquisition of energy for replication and capsid assembly. This is the first study describing the chemical profiling of a polar extract obtained from Capsicum annuum infected by BMCTV under open field conditions.",
author = "Nemesio Villa-Ruano and Rodolfo Vel{\'a}squez-Valle and Zepeda-Vallejo, {L. Gerardo} and Nury P{\'e}rez-Hern{\'a}ndez and Manuel Vel{\'a}zquez-Ponce and Arcos-Adame, {Victor M.} and Elvia Becerra-Mart{\'i}nez",
year = "2018",
month = "4",
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doi = "10.1016/j.foodres.2018.01.065",
language = "American English",
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T1 - 1H NMR-based metabolomic profiling for identification of metabolites in Capsicum annuum cv. mirasol infected by beet mild curly top virus (BMCTV)

AU - Villa-Ruano, Nemesio

AU - Velásquez-Valle, Rodolfo

AU - Zepeda-Vallejo, L. Gerardo

AU - Pérez-Hernández, Nury

AU - Velázquez-Ponce, Manuel

AU - Arcos-Adame, Victor M.

AU - Becerra-Martínez, Elvia

PY - 2018/4/1

Y1 - 2018/4/1

N2 - © 2018 Elsevier Ltd Beet mild curly top virus (BMCTV) is associated with an outbreak of curly top in chili pepper, tomato and other Solanaceae species, which can cause severe crop losses. The aim of this work was to obtain the 1H NMR metabolomic profiling of both healthy chili peppers (cv. mirasol) and infected chili peppers with BMCTV in order to find chemical markers associated to the infection process. Significant differences were found between the two groups, according to principal component analysis and orthogonal projections to latent structure discriminant analysis. Compared to the asymptomatic peppers, the symptomatic fruits had higher relative abundance of fructose, isoleucine, histidine, phenylalanine and tryptophan. Contrarily, the asymptomatic samples showed greater amounts of malonate and isobutyrate. These results suggest that in diseased chili peppers there are metabolic changes related to the viral acquisition of energy for replication and capsid assembly. This is the first study describing the chemical profiling of a polar extract obtained from Capsicum annuum infected by BMCTV under open field conditions.

AB - © 2018 Elsevier Ltd Beet mild curly top virus (BMCTV) is associated with an outbreak of curly top in chili pepper, tomato and other Solanaceae species, which can cause severe crop losses. The aim of this work was to obtain the 1H NMR metabolomic profiling of both healthy chili peppers (cv. mirasol) and infected chili peppers with BMCTV in order to find chemical markers associated to the infection process. Significant differences were found between the two groups, according to principal component analysis and orthogonal projections to latent structure discriminant analysis. Compared to the asymptomatic peppers, the symptomatic fruits had higher relative abundance of fructose, isoleucine, histidine, phenylalanine and tryptophan. Contrarily, the asymptomatic samples showed greater amounts of malonate and isobutyrate. These results suggest that in diseased chili peppers there are metabolic changes related to the viral acquisition of energy for replication and capsid assembly. This is the first study describing the chemical profiling of a polar extract obtained from Capsicum annuum infected by BMCTV under open field conditions.

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