1 H NMR-based fingerprinting of eleven Mexican Capsicum annuum cultivars

Elideth Florentino-Ramos, Nemesio Villa-Ruano, Diego Hidalgo-Martínez, Moisés Ramírez-Meraz, Reinaldo Méndez-Aguilar, Rodolfo Velásquez-Valle, L. Gerardo Zepeda-Vallejo, Nury Pérez-Hernández, Elvia Becerra-Martínez

Research output: Contribution to journalArticleResearchpeer-review

Abstract

© 2019 Elsevier Ltd Approximately 90% of the chili peppers consumed in the world are harvested in Mexico. The present article describes the untargeted 1 H NMR-based metabolomic profiling of 11 cultivars of Capsicum annuum species which are routinely consumed worldwide. The metabolomic fingerprinting detected via 1 H NMR contained 44 metabolites including sugars, amino acids, organic acids, polyphenolic acids and alcohols which were identified by comparison with the literature data, with Chenomx database and by 2D NMR. Statistical approaches based on principal component analysis (PCA) and linear discriminant analysis (LDA) were used to classify the Capsicum annuum cultivars according to their metabolite profile. LDA revealed metabolomic differences and similarities among Capsicum annuum cultivars, whereas hierarchical cluster analysis (HCA) significantly separated the cultivars according to the phylogenetic trees obtained. Substantial endogenous levels of free amino acids and carbohydrates were detected in all the studied cultivars but interestingly, Capsicum annuum cv. mirasol and C. annuum cv. chilaca contained almost three-fold more endogenous levels of vitamin C than the other cultivars. Considering that this antioxidant was found in crude aqueous extracts, its abundance could be directly proportional to its bioavailability for human nutrition. The results suggest that 1 H NMR is an effective method to determine differences among cultivars of the Capsicum annuum species.
Original languageAmerican English
Pages (from-to)12-19
Number of pages10
JournalFood Research International
DOIs
StatePublished - 1 Jul 2019

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Capsicum
Capsicum annuum
cultivars
Metabolomics
metabolomics
Discriminant Analysis
discriminant analysis
metabolites
Amino Acids
Sugar Acids
Acids
hot peppers
human nutrition
Mexico
Principal Component Analysis
Complex Mixtures
free amino acids
Biological Availability
Ascorbic Acid
organic acids and salts

Cite this

Florentino-Ramos, E., Villa-Ruano, N., Hidalgo-Martínez, D., Ramírez-Meraz, M., Méndez-Aguilar, R., Velásquez-Valle, R., ... Becerra-Martínez, E. (2019). 1 H NMR-based fingerprinting of eleven Mexican Capsicum annuum cultivars. Food Research International, 12-19. https://doi.org/10.1016/j.foodres.2019.03.025
Florentino-Ramos, Elideth ; Villa-Ruano, Nemesio ; Hidalgo-Martínez, Diego ; Ramírez-Meraz, Moisés ; Méndez-Aguilar, Reinaldo ; Velásquez-Valle, Rodolfo ; Zepeda-Vallejo, L. Gerardo ; Pérez-Hernández, Nury ; Becerra-Martínez, Elvia. / 1 H NMR-based fingerprinting of eleven Mexican Capsicum annuum cultivars. In: Food Research International. 2019 ; pp. 12-19.
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abstract = "{\circledC} 2019 Elsevier Ltd Approximately 90{\%} of the chili peppers consumed in the world are harvested in Mexico. The present article describes the untargeted 1 H NMR-based metabolomic profiling of 11 cultivars of Capsicum annuum species which are routinely consumed worldwide. The metabolomic fingerprinting detected via 1 H NMR contained 44 metabolites including sugars, amino acids, organic acids, polyphenolic acids and alcohols which were identified by comparison with the literature data, with Chenomx database and by 2D NMR. Statistical approaches based on principal component analysis (PCA) and linear discriminant analysis (LDA) were used to classify the Capsicum annuum cultivars according to their metabolite profile. LDA revealed metabolomic differences and similarities among Capsicum annuum cultivars, whereas hierarchical cluster analysis (HCA) significantly separated the cultivars according to the phylogenetic trees obtained. Substantial endogenous levels of free amino acids and carbohydrates were detected in all the studied cultivars but interestingly, Capsicum annuum cv. mirasol and C. annuum cv. chilaca contained almost three-fold more endogenous levels of vitamin C than the other cultivars. Considering that this antioxidant was found in crude aqueous extracts, its abundance could be directly proportional to its bioavailability for human nutrition. The results suggest that 1 H NMR is an effective method to determine differences among cultivars of the Capsicum annuum species.",
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Florentino-Ramos, E, Villa-Ruano, N, Hidalgo-Martínez, D, Ramírez-Meraz, M, Méndez-Aguilar, R, Velásquez-Valle, R, Zepeda-Vallejo, LG, Pérez-Hernández, N & Becerra-Martínez, E 2019, '1 H NMR-based fingerprinting of eleven Mexican Capsicum annuum cultivars', Food Research International, pp. 12-19. https://doi.org/10.1016/j.foodres.2019.03.025

1 H NMR-based fingerprinting of eleven Mexican Capsicum annuum cultivars. / Florentino-Ramos, Elideth; Villa-Ruano, Nemesio; Hidalgo-Martínez, Diego; Ramírez-Meraz, Moisés; Méndez-Aguilar, Reinaldo; Velásquez-Valle, Rodolfo; Zepeda-Vallejo, L. Gerardo; Pérez-Hernández, Nury; Becerra-Martínez, Elvia.

In: Food Research International, 01.07.2019, p. 12-19.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - 1 H NMR-based fingerprinting of eleven Mexican Capsicum annuum cultivars

AU - Florentino-Ramos, Elideth

AU - Villa-Ruano, Nemesio

AU - Hidalgo-Martínez, Diego

AU - Ramírez-Meraz, Moisés

AU - Méndez-Aguilar, Reinaldo

AU - Velásquez-Valle, Rodolfo

AU - Zepeda-Vallejo, L. Gerardo

AU - Pérez-Hernández, Nury

AU - Becerra-Martínez, Elvia

PY - 2019/7/1

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N2 - © 2019 Elsevier Ltd Approximately 90% of the chili peppers consumed in the world are harvested in Mexico. The present article describes the untargeted 1 H NMR-based metabolomic profiling of 11 cultivars of Capsicum annuum species which are routinely consumed worldwide. The metabolomic fingerprinting detected via 1 H NMR contained 44 metabolites including sugars, amino acids, organic acids, polyphenolic acids and alcohols which were identified by comparison with the literature data, with Chenomx database and by 2D NMR. Statistical approaches based on principal component analysis (PCA) and linear discriminant analysis (LDA) were used to classify the Capsicum annuum cultivars according to their metabolite profile. LDA revealed metabolomic differences and similarities among Capsicum annuum cultivars, whereas hierarchical cluster analysis (HCA) significantly separated the cultivars according to the phylogenetic trees obtained. Substantial endogenous levels of free amino acids and carbohydrates were detected in all the studied cultivars but interestingly, Capsicum annuum cv. mirasol and C. annuum cv. chilaca contained almost three-fold more endogenous levels of vitamin C than the other cultivars. Considering that this antioxidant was found in crude aqueous extracts, its abundance could be directly proportional to its bioavailability for human nutrition. The results suggest that 1 H NMR is an effective method to determine differences among cultivars of the Capsicum annuum species.

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Florentino-Ramos E, Villa-Ruano N, Hidalgo-Martínez D, Ramírez-Meraz M, Méndez-Aguilar R, Velásquez-Valle R et al. 1 H NMR-based fingerprinting of eleven Mexican Capsicum annuum cultivars. Food Research International. 2019 Jul 1;12-19. https://doi.org/10.1016/j.foodres.2019.03.025