Sulforaphane protects against cisplatin-induced nephrotoxicity

Carlos Enrique Guerrero-Beltrán, Mariel Calderón-Oliver, Edilia Tapia, Omar N. Medina-Campos, Dolores Javier Sánchez-González, Claudia María Martínez-Martínez, Karla Mariana Ortiz-Vega, Martha Franco, José Pedraza-Chaverri

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Cisplatin (cis-diamminedichloroplatinum II, CDDP) is a chemotherapeutic agent that induces nephrotoxicity associated with oxidative/nitrosative stress. Sulforaphane (SFN) is an isothiocyanate produced by the enzymatic action of myrosinase on glucorophanin, a glucosinolate contained in cruciferous vegetables. SFN is able to induce cytoprotective enzymes through the transcription factor Nrf2. The purpose of this study was to evaluate whether SFN induces a cytoprotective effect on the CDDP-induced nephrotoxicity. Preincubation of LLC-PK1 cells with 0.5-5 μM SFN by 24 h was able to prevent, in a concentration-dependent way, CDDP-induced cell death. Immunofluorescent staining confirmed the nuclear translocation of Nrf2 after treatment with SFN. In the in vivo studies, CDDP was given to Wistar rats as a sole i.p. injection at a dose of 7.5 mg/kg. SFN (500 μg/kg i.v.) was given two times (24 h before and 24 after CDDP-injection). Animals were killed three days after CDDP-injection. SFN attenuated CDDP-induced renal dysfunction, structural damage, oxidative/nitrosative stress, glutathione depletion, enhanced urinary hydrogen peroxide excretion and the decrease in antioxidant enzymes (catalase, glutathione peroxidase and glutathione-S-transferase). The renoprotective effect of SFN on CDDP-induced nephrotoxicity was associated with the attenuation in oxidative/nitrosative stress and the preservation of antioxidant enzymes.

Original languageEnglish
Pages (from-to)278-285
Number of pages8
JournalToxicology Letters
Issue number3
StatePublished - 15 Feb 2010
Externally publishedYes


  • Cisplatin
  • Nephrotoxicity
  • Nrf2
  • Oxidative/nitrosative stress
  • Renoprotection
  • Sulforaphane


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