TY - JOUR
T1 - Study of the binding between lysozyme and C10-TAB
T2 - Determination and interpretation of the partial properties of protein and surfactant at infinite dilution
AU - Morgado, Jorge
AU - Aquino-Olivos, Marco Antonio
AU - Martínez-Hernández, Ranulfo
AU - Corea, Mónica
AU - Grolier, Jean Pierre E.
AU - del Río, José Manuel
PY - 2008/6
Y1 - 2008/6
N2 - This work examines the binding in aqueous solution, through the experimental determination of specific volumes and specific adiabatic compressibility coefficients, of decyltrimethylammonium bromide to lysozyme and to non-charged polymeric particles (which have been specially synthesized by emulsion polymerization). A method was developed to calculate the specific partial properties at infinite dilution and it was shown that a Gibbs-Duhem type equation holds at this limit for two solutes. With this equation, it is possible to relate the behavior of the partial properties along different binding types at a constant temperature. It was found that the first binding type, specific with high affinity, is related to a significant reduction of surfactant compressibility. The second binding type is accompanied by the unfolding of the protein and the third one is qualitatively identical to the binding of the surfactant to non-charged polymeric particles.
AB - This work examines the binding in aqueous solution, through the experimental determination of specific volumes and specific adiabatic compressibility coefficients, of decyltrimethylammonium bromide to lysozyme and to non-charged polymeric particles (which have been specially synthesized by emulsion polymerization). A method was developed to calculate the specific partial properties at infinite dilution and it was shown that a Gibbs-Duhem type equation holds at this limit for two solutes. With this equation, it is possible to relate the behavior of the partial properties along different binding types at a constant temperature. It was found that the first binding type, specific with high affinity, is related to a significant reduction of surfactant compressibility. The second binding type is accompanied by the unfolding of the protein and the third one is qualitatively identical to the binding of the surfactant to non-charged polymeric particles.
KW - Binding
KW - Compressibility
KW - Hydration
KW - Partial volume
KW - Polymeric particle
KW - Protein
UR - http://www.scopus.com/inward/record.url?scp=43049168788&partnerID=8YFLogxK
U2 - 10.1016/j.bpc.2008.03.002
DO - 10.1016/j.bpc.2008.03.002
M3 - Artículo
C2 - 18433980
SN - 0301-4622
VL - 135
SP - 51
EP - 58
JO - Biophysical Chemistry
JF - Biophysical Chemistry
IS - 1-3
ER -