TY - JOUR
T1 - STUDY of INDOLE-3-ACETIC ACID BIOSYNTHESIS PATHWAYS in Bradyrhizobium japonicum BJBV-05
AU - González, Saida Marisol Capuchina
AU - Rodríguezcastillejos, Guadalupe
AU - Lizarazo-Ortega, Cristian
AU - Sánchez-Yáñez, Juan Manuel
AU - Cano, Efrén Garza
AU - Oliva-Hernández, Amanda Alejandra
AU - Jiménez, María Cristina Hernández
AU - Hernández-Mendoza, José Luis
N1 - Publisher Copyright:
© 2021 Interciencia Association. All rights reserved.
PY - 2021/5
Y1 - 2021/5
N2 - The bacterium Bradyrhizobium japonicum induces nodulation in Glycine max (soy) plants and other legumes. It is considered of great importance, since it is in the nodules that the bacteria are established, contributing to the biological fixation of N2. The process is controlled by nitrogenase, an enzyme produced by the nif genes present in the genome of the bacterium. By metabolizing the nitrogenase, the indole acetonitrile transforms it into indole acetic acid (IAA) and releases a nitrogenated molecule. There have been other IAA synthesis routes reported in plants, other genera and species of bacteria, fungi, and algae, named tryptophan-dependent (TRP-D) or tryptophan independent (TRP-I), where this amino acid is the precursor. For TRP-D, there are four pathways to the synthesis of IAA, and only two for TRP-I. The microorganisms may or not have all the routes in their genomes, and the expression of the genes varies with the isolation and the genotype of the host plant. This work reports the results obtained from an B. japonicum soy isolate, cultivated in an enriched LB medium, or alternatively, with tryptophan. With the data obtained, we estimate that B. japonicum uses both TRP-D and TRP-I routes, since in the former type indole acetamide was detected, and in the latter, indole and anthranilic acid were found. Likewise, the presence of TRP in the medium may alter IAA synthesis routes.
AB - The bacterium Bradyrhizobium japonicum induces nodulation in Glycine max (soy) plants and other legumes. It is considered of great importance, since it is in the nodules that the bacteria are established, contributing to the biological fixation of N2. The process is controlled by nitrogenase, an enzyme produced by the nif genes present in the genome of the bacterium. By metabolizing the nitrogenase, the indole acetonitrile transforms it into indole acetic acid (IAA) and releases a nitrogenated molecule. There have been other IAA synthesis routes reported in plants, other genera and species of bacteria, fungi, and algae, named tryptophan-dependent (TRP-D) or tryptophan independent (TRP-I), where this amino acid is the precursor. For TRP-D, there are four pathways to the synthesis of IAA, and only two for TRP-I. The microorganisms may or not have all the routes in their genomes, and the expression of the genes varies with the isolation and the genotype of the host plant. This work reports the results obtained from an B. japonicum soy isolate, cultivated in an enriched LB medium, or alternatively, with tryptophan. With the data obtained, we estimate that B. japonicum uses both TRP-D and TRP-I routes, since in the former type indole acetamide was detected, and in the latter, indole and anthranilic acid were found. Likewise, the presence of TRP in the medium may alter IAA synthesis routes.
KW - / Biosynthesis
KW - Indole Acetamide
KW - Indole Acetonitrile
KW - Tryptophan Independent /
KW - Tryptophan-Dependent
UR - http://www.scopus.com/inward/record.url?scp=85107751156&partnerID=8YFLogxK
M3 - Artículo de revisión
AN - SCOPUS:85107751156
SN - 0378-1844
VL - 46
SP - 198
EP - 203
JO - Interciencia
JF - Interciencia
IS - 5
ER -