TY - GEN
T1 - Study of a Denatured Bovine Serum Albumin Solution Used as Lubricant in Tribological Testing of Total Knee Replacements
AU - Girón de la Cruz, G. I.
AU - Barceinas-Sánchez, J. D.O.
AU - Gómez-Ramírez, M.
N1 - Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Nature Switzerland AG.
PY - 2022
Y1 - 2022
N2 - The lubricant solution used in tribological testing of total knee replacements must have a 20 g/L (ISO 14243-3:2014) overall value of protein concentration; bovine serums (BSs) are currently used as base fluids to prepare such solution. BSs normally have a higher concentration of bovine serum albumin (BSA) and minor concentration of globulins (α, β and γ). It has been observed that these proteins can be degraded and adsorbed onto the surface of the materials during tribological tests. Therefore, this research is aimed at analyzing the proteins of denatured lubricant BSA-based solutions having a protein concentration of 20 g/L, after 6 h of tribological testing under different conditions. The testing tribological parameters considered were the applied load (L), sliding-to-rolling ratio (SRR), and entrainment speed (Vm). Four combination of parameters were set: (a) 8N-18-80 mm/s, (b) 2N-18-80 mm/s, (c) 8N-0.2-20 mm/s, and (d) 2N-0.2-20 mm/s. All tests carried out at 37 °C. Samples of the solutions were analyzed before and after testing by UV–Vis spectroscopy (range 200–300 nm), Bradford and Ellman methods. The UV–Vis spectroscopy analyzes allowed to determine possible change suffered of the aromatic residues (residues exposed or covered cause an increase or decrease of absorbance). Bradford analysis of solutions indicated similar content of proteins. Therefore, basic amino acid residues in proteins interact and reorganize. Moreover, disulfide bonds involved in tertiary structure of protein remains stable. Finally, COF (Coefficient friction) is dependent on both, protein state as well as the tribological parameters.
AB - The lubricant solution used in tribological testing of total knee replacements must have a 20 g/L (ISO 14243-3:2014) overall value of protein concentration; bovine serums (BSs) are currently used as base fluids to prepare such solution. BSs normally have a higher concentration of bovine serum albumin (BSA) and minor concentration of globulins (α, β and γ). It has been observed that these proteins can be degraded and adsorbed onto the surface of the materials during tribological tests. Therefore, this research is aimed at analyzing the proteins of denatured lubricant BSA-based solutions having a protein concentration of 20 g/L, after 6 h of tribological testing under different conditions. The testing tribological parameters considered were the applied load (L), sliding-to-rolling ratio (SRR), and entrainment speed (Vm). Four combination of parameters were set: (a) 8N-18-80 mm/s, (b) 2N-18-80 mm/s, (c) 8N-0.2-20 mm/s, and (d) 2N-0.2-20 mm/s. All tests carried out at 37 °C. Samples of the solutions were analyzed before and after testing by UV–Vis spectroscopy (range 200–300 nm), Bradford and Ellman methods. The UV–Vis spectroscopy analyzes allowed to determine possible change suffered of the aromatic residues (residues exposed or covered cause an increase or decrease of absorbance). Bradford analysis of solutions indicated similar content of proteins. Therefore, basic amino acid residues in proteins interact and reorganize. Moreover, disulfide bonds involved in tertiary structure of protein remains stable. Finally, COF (Coefficient friction) is dependent on both, protein state as well as the tribological parameters.
KW - Bovine serum albumin
KW - Denaturation
KW - Tribological testing
UR - http://www.scopus.com/inward/record.url?scp=85119876380&partnerID=8YFLogxK
U2 - 10.1007/978-3-030-82064-0_7
DO - 10.1007/978-3-030-82064-0_7
M3 - Contribución a la conferencia
AN - SCOPUS:85119876380
SN - 9783030820633
T3 - Lecture Notes in Networks and Systems
SP - 79
EP - 91
BT - Recent Trends in Sustainable Engineering - Proceedings of the 2nd International Conference on Applied Science and Advanced Technology
A2 - Flores Rodríguez, Karen Lizbeth
A2 - Ramos Alvarado, Raymundo
A2 - Barati, Masoud
A2 - Segovia Tagle, Veronica
A2 - Velázquez González, Roberto Sostrand
PB - Springer Science and Business Media Deutschland GmbH
T2 - 2nd International Conference on Applied Science and Advanced Technology, ICASAT 2021
Y2 - 2 June 2021 through 3 June 2021
ER -