Structural diversity of class 1 integrons in multiresistant strains of Escherichia coli isolated from patients in a hospital in Mexico city

Gabriel Acosta-Pérez, Gabriela Ibáñez-Cervantes, Juan Manuel Bello-López, José Manuel Hernández, Zahuiti Hernández-Montañez, Silvia Giono-Cerezo, Gregorio León-García, Gloria León-Avila

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

© Springer Science+Business Media New York 2015. Since a decade, Escherichia coli has been considered an important nosocomial pathogen due to the high number of isolates multiresistant to antimicrobials reported worldwide. In clinical and environmental strains, transposons, plasmids, and integrons are currently considered the principal genetic elements responsible for the acquisition of antibiotic resistance through horizontal transfer. The objective of this research was to correlate the resistance to antibiotics of E. coli clinical strains with the presence class I integrons. In the present study, one hundred E. coli strains were isolated and tested for susceptibility and resistance to antimicrobials. Class 1 integrons were detected by PCR, and the arrangement of gene cassettes was determined by sequencing. Twenty two strains were found to carry Class 1 integrons. Sequence analysis of the variable regions revealed the presence of several gene cassettes, such as dihydrofolate reductases (dfr2d, dfrA17, and dhfrXVb), adenylyl transferases (aadA2, addA5, and addA22), and chloramphenicol efflux pump (cmlA), and oxacillinase (blaOXA–1). The dfrA17–addA5 arrangement prevailed upon other integrons in the study. This is the first report of the presence of the dfr2d and dhfrXVb–aadA2 cassette arrangements in a Class 1 integrons from clinical strains of E. coli. In most of the strains, it was found a direct relationship between genetic arrangements and resistance phenotypes. Four integrons were detected in plasmids that might be involved in the resistance genes transfer to other bacteria of clinical importance. Our results confirm the presence of Class 1 integrons and their essential role in the dissemination of resistance cassettes among E. coli strains.
Original languageAmerican English
Pages (from-to)501-508
Number of pages450
JournalCurrent Microbiology
DOIs
StatePublished - 1 Oct 2015

Fingerprint

Integrons
Mexico
Escherichia coli
Microbial Drug Resistance
Plasmids
Tetrahydrofolate Dehydrogenase
Gene Order
Chloramphenicol
Genes
Sequence Analysis
Bacteria
Phenotype
Polymerase Chain Reaction

Cite this

Acosta-Pérez, Gabriel ; Ibáñez-Cervantes, Gabriela ; Bello-López, Juan Manuel ; Hernández, José Manuel ; Hernández-Montañez, Zahuiti ; Giono-Cerezo, Silvia ; León-García, Gregorio ; León-Avila, Gloria. / Structural diversity of class 1 integrons in multiresistant strains of Escherichia coli isolated from patients in a hospital in Mexico city. In: Current Microbiology. 2015 ; pp. 501-508.
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abstract = "{\circledC} Springer Science+Business Media New York 2015. Since a decade, Escherichia coli has been considered an important nosocomial pathogen due to the high number of isolates multiresistant to antimicrobials reported worldwide. In clinical and environmental strains, transposons, plasmids, and integrons are currently considered the principal genetic elements responsible for the acquisition of antibiotic resistance through horizontal transfer. The objective of this research was to correlate the resistance to antibiotics of E. coli clinical strains with the presence class I integrons. In the present study, one hundred E. coli strains were isolated and tested for susceptibility and resistance to antimicrobials. Class 1 integrons were detected by PCR, and the arrangement of gene cassettes was determined by sequencing. Twenty two strains were found to carry Class 1 integrons. Sequence analysis of the variable regions revealed the presence of several gene cassettes, such as dihydrofolate reductases (dfr2d, dfrA17, and dhfrXVb), adenylyl transferases (aadA2, addA5, and addA22), and chloramphenicol efflux pump (cmlA), and oxacillinase (blaOXA–1). The dfrA17–addA5 arrangement prevailed upon other integrons in the study. This is the first report of the presence of the dfr2d and dhfrXVb–aadA2 cassette arrangements in a Class 1 integrons from clinical strains of E. coli. In most of the strains, it was found a direct relationship between genetic arrangements and resistance phenotypes. Four integrons were detected in plasmids that might be involved in the resistance genes transfer to other bacteria of clinical importance. Our results confirm the presence of Class 1 integrons and their essential role in the dissemination of resistance cassettes among E. coli strains.",
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Structural diversity of class 1 integrons in multiresistant strains of Escherichia coli isolated from patients in a hospital in Mexico city. / Acosta-Pérez, Gabriel; Ibáñez-Cervantes, Gabriela; Bello-López, Juan Manuel; Hernández, José Manuel; Hernández-Montañez, Zahuiti; Giono-Cerezo, Silvia; León-García, Gregorio; León-Avila, Gloria.

In: Current Microbiology, 01.10.2015, p. 501-508.

Research output: Contribution to journalArticle

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AU - Acosta-Pérez, Gabriel

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AU - Hernández, José Manuel

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