TY - JOUR
T1 - Somatic embryogenesis from leaf explants of Tagetes erecta L
AU - Espinoza, Pablo Emilio Vanegas
AU - Benítez-García, Israel
AU - Peralta, Annel Lizeth Leyva
AU - Paredes-López, Octavio
AU - Del Villar-Martínez, Alma Angélica
N1 - Publisher Copyright:
© 2017 The Japanese Society for Plant Cell and Molecular Biology.
PY - 2017
Y1 - 2017
N2 - Tagetes erecta is an asteraceous plant of industrial, ornamental and medicinal importance; its inflorescences have been used as a pigment source for food coloring, mainly for poultry skin and eggs. Nevertheless, there are few reports on plant regeneration or micropropagation, because unsuccesfull results in the plant’s reaction to the growth regulators, developing embryogenesis on Tagetes erecta. In this study, somatic embryogenesis was induced and plantlets of Tagetes erecta were regenerated. For induction of globular structures MS medium supplemented with 2,4-D (4.5 µM) and BAP (8.8 µM) was used; globular structures were transferred to MS medium with 45 g l−1 sucrose until the embryos maturation. Transmission electron microscopy showed characteristic subcellular structures of embryogenic callus. Somatic embryos were transferred to MS medium without plant growth regulators and whole plantlets were obtained. In vitro plants were successfully transplanted into a mixture of peat moss and vermiculite (1 : 1 v/v) under greenhouse conditions. In this study, somatic embryogenesis and plant regeneration system from foliar explants were established, an important requirement for performing genetic transformation events on Tagetes erecta.
AB - Tagetes erecta is an asteraceous plant of industrial, ornamental and medicinal importance; its inflorescences have been used as a pigment source for food coloring, mainly for poultry skin and eggs. Nevertheless, there are few reports on plant regeneration or micropropagation, because unsuccesfull results in the plant’s reaction to the growth regulators, developing embryogenesis on Tagetes erecta. In this study, somatic embryogenesis was induced and plantlets of Tagetes erecta were regenerated. For induction of globular structures MS medium supplemented with 2,4-D (4.5 µM) and BAP (8.8 µM) was used; globular structures were transferred to MS medium with 45 g l−1 sucrose until the embryos maturation. Transmission electron microscopy showed characteristic subcellular structures of embryogenic callus. Somatic embryos were transferred to MS medium without plant growth regulators and whole plantlets were obtained. In vitro plants were successfully transplanted into a mixture of peat moss and vermiculite (1 : 1 v/v) under greenhouse conditions. In this study, somatic embryogenesis and plant regeneration system from foliar explants were established, an important requirement for performing genetic transformation events on Tagetes erecta.
KW - Asteraceae
KW - Embryo stages
KW - In vitro culture
KW - Plant regeneration
UR - http://www.scopus.com/inward/record.url?scp=85042377233&partnerID=8YFLogxK
U2 - 10.5511/plantbiotechnology.17.1120a
DO - 10.5511/plantbiotechnology.17.1120a
M3 - Artículo
SN - 1342-4580
VL - 34
SP - 187
EP - 192
JO - Plant Biotechnology
JF - Plant Biotechnology
IS - 4
ER -