Sodium butyrate increases the effect of the photodynamic therapy: A mechanism that involves modulation of gene expression and differentiation in astrocytoma cells

José Bueno-Carrazco, Violeta Castro-Leyva, Fanny García-Gomez, Mario Solís-Paredes, Eva Ramon-Gallegos, Alfredo Cruz-Orea, Pilar Eguía-Aguilar, Francisco Arenas-Huertero

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Objectives In order to evaluate the improvement of the photodynamic therapy (PDT) due to sodium butyrate (NaBu), its effectiveness in U373-MG and D54-MG astrocytoma cell lines was evaluated. Methods Cells were exposed to delta-aminolevulinic acid (δ-ALA) as a precursor to endogenous photosensitizer protoporphyrin IX (PpIX). In both astrocytoma cells, an important increase by ALA was observed in uroporphyrinogen synthetase gene expression: 1.8- and 52-fold for D54-MG and U373-MG cells, respectively. After irradiation, they showed 16.67 and 28.9 % of mortality in U373-MG and D54-MG, respectively. These mortalities increased to 70.62 and 96.7 % when U373-MG and D54-MG cells, respectively, were exposed 24 h to 8 mM NaBu, before to PpIX induction. NaBu induced expression of caspase-3, caspase-9, and Bcl-2 and increased Bax in U373-MG cells. ALA-induced morphological changes are compatible to differentiation. Conclusions Genes and differentiation induced mainly by NaBu improve cell death performed by PDT in astrocytoma cells. These facts prove the synergistic effect of NaBu on cytotoxic damage induced by PDT. ©Springer-Verlag 2012.
Original languageAmerican English
Pages (from-to)1723-1730
Number of pages1549
JournalChild's Nervous System
DOIs
StatePublished - 1 Oct 2012
Externally publishedYes

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