TY - JOUR
T1 - Sodium butyrate increases the effect of the photodynamic therapy
T2 - A mechanism that involves modulation of gene expression and differentiation in astrocytoma cells
AU - Bueno-Carrazco, José
AU - Castro-Leyva, Violeta
AU - García-Gomez, Fanny
AU - Solís-Paredes, Mario
AU - Ramon-Gallegos, Eva
AU - Cruz-Orea, Alfredo
AU - Eguía-Aguilar, Pilar
AU - Arenas-Huertero, Francisco
N1 - Funding Information:
Acknowledgments Financing for this study was provided by Sistema de Investigación y Posgrado through project 20070480 under the direction of Eva Ramon-Gallegos. ERG is Sistema Nacional de Investigadores (SNI), Comisión Operadora para el Fomentos a las Actividades Aca-démicas and Estímulo al Desarrollo de la Investigación fellow. F A-H is a member of the Sistema Nacional de Investigadores (SNI-I).
PY - 2012/10
Y1 - 2012/10
N2 - Objectives In order to evaluate the improvement of the photodynamic therapy (PDT) due to sodium butyrate (NaBu), its effectiveness in U373-MG and D54-MG astrocytoma cell lines was evaluated. Methods Cells were exposed to delta-aminolevulinic acid (δ-ALA) as a precursor to endogenous photosensitizer protoporphyrin IX (PpIX). In both astrocytoma cells, an important increase by ALA was observed in uroporphyrinogen synthetase gene expression: 1.8- and 52-fold for D54-MG and U373-MG cells, respectively. After irradiation, they showed 16.67 and 28.9 % of mortality in U373-MG and D54-MG, respectively. These mortalities increased to 70.62 and 96.7 % when U373-MG and D54-MG cells, respectively, were exposed 24 h to 8 mM NaBu, before to PpIX induction. NaBu induced expression of caspase-3, caspase-9, and Bcl-2 and increased Bax in U373-MG cells. ALA-induced morphological changes are compatible to differentiation. Conclusions Genes and differentiation induced mainly by NaBu improve cell death performed by PDT in astrocytoma cells. These facts prove the synergistic effect of NaBu on cytotoxic damage induced by PDT.
AB - Objectives In order to evaluate the improvement of the photodynamic therapy (PDT) due to sodium butyrate (NaBu), its effectiveness in U373-MG and D54-MG astrocytoma cell lines was evaluated. Methods Cells were exposed to delta-aminolevulinic acid (δ-ALA) as a precursor to endogenous photosensitizer protoporphyrin IX (PpIX). In both astrocytoma cells, an important increase by ALA was observed in uroporphyrinogen synthetase gene expression: 1.8- and 52-fold for D54-MG and U373-MG cells, respectively. After irradiation, they showed 16.67 and 28.9 % of mortality in U373-MG and D54-MG, respectively. These mortalities increased to 70.62 and 96.7 % when U373-MG and D54-MG cells, respectively, were exposed 24 h to 8 mM NaBu, before to PpIX induction. NaBu induced expression of caspase-3, caspase-9, and Bcl-2 and increased Bax in U373-MG cells. ALA-induced morphological changes are compatible to differentiation. Conclusions Genes and differentiation induced mainly by NaBu improve cell death performed by PDT in astrocytoma cells. These facts prove the synergistic effect of NaBu on cytotoxic damage induced by PDT.
KW - Acetylation
KW - Gliomas .Deathcell .Cellculture .RNA HDAC
KW - Laser
UR - http://www.scopus.com/inward/record.url?scp=84867582398&partnerID=8YFLogxK
U2 - 10.1007/s00381-012-1828-3
DO - 10.1007/s00381-012-1828-3
M3 - Artículo
C2 - 22710635
SN - 0256-7040
VL - 28
SP - 1723
EP - 1730
JO - Child's Nervous System
JF - Child's Nervous System
IS - 10
ER -