TY - JOUR
T1 - Sindbis virus-based measles DNA vaccines protect cotton rats against respiratory measles
T2 - Relevance of antibodies, mucosal and systemic antibody-secreting cells, memory B cells, and Th1-type cytokines as correlates of immunity
AU - Pasetti, Marcela F.
AU - Ramirez, Karina
AU - Resendiz-Albor, Aldo
AU - Ulmer, Jeffrey
AU - Barry, Eileen M.
AU - Levine, Myron M.
PY - 2009/3
Y1 - 2009/3
N2 - Measles remains an important cause of pediatric morbidity and mortality in developing countries, especially among infants who are too young to receive the current licensed live attenuated measles vaccine. We developed two Sindbis virus DNA vaccines encoding the measles virus hemagglutinin (pMSIN-H) and fusion proteins (pMSINH-FdU) and examined their immunogenicities and protective efficacies when administered alone or followed by the live measles virus vaccine in cotton rats. Neutralizing antibodies, mucosal and systemic antibody-secreting cells, memory B cells, and gamma interferon-secreting T cells developed after priming and increased after boosting. pMSIN-H priming conferred 100% protection against pulmonary measles, whereas pMSINH-FdU protected only in conjunction with the live measles virus vaccine boost.
AB - Measles remains an important cause of pediatric morbidity and mortality in developing countries, especially among infants who are too young to receive the current licensed live attenuated measles vaccine. We developed two Sindbis virus DNA vaccines encoding the measles virus hemagglutinin (pMSIN-H) and fusion proteins (pMSINH-FdU) and examined their immunogenicities and protective efficacies when administered alone or followed by the live measles virus vaccine in cotton rats. Neutralizing antibodies, mucosal and systemic antibody-secreting cells, memory B cells, and gamma interferon-secreting T cells developed after priming and increased after boosting. pMSIN-H priming conferred 100% protection against pulmonary measles, whereas pMSINH-FdU protected only in conjunction with the live measles virus vaccine boost.
UR - http://www.scopus.com/inward/record.url?scp=62749177292&partnerID=8YFLogxK
U2 - 10.1128/JVI.02191-08
DO - 10.1128/JVI.02191-08
M3 - Artículo
SN - 0022-538X
VL - 83
SP - 2789
EP - 2794
JO - Journal of Virology
JF - Journal of Virology
IS - 6
ER -