TY - JOUR
T1 - Serum Dried Samples to Detect Dengue Antibodies
T2 - A Field Study
AU - Maldonado-Rodríguez, Angelica
AU - Rojas-Montes, Othon
AU - Vazquez-Rosales, Guillermo
AU - Chavez-Negrete, Adolfo
AU - Rojas-Uribe, Magdalena
AU - Posadas-Mondragon, Araceli
AU - Aguilar-Faisal, Leopoldo
AU - Cevallos, Ana Maria
AU - Xoconostle-Cazares, Beatriz
AU - Lira, Rosalia
N1 - Publisher Copyright:
© 2017 Angelica Maldonado-Rodríguez et al.
PY - 2017
Y1 - 2017
N2 - Background. Dried blood and serum samples are useful resources for detecting antiviral antibodies. The conditions for elution of the sample need to be optimized for each disease. Dengue is a widespread disease in Mexico which requires continuous surveillance. In this study, we standardized and validated a protocol for the specific detection of dengue antibodies from dried serum spots (DSSs). Methods. Paired serum and DSS samples from 66 suspected cases of dengue were collected in a clinic in Veracruz, Mexico. Samples were sent to our laboratory, where the conditions for optimal elution of DSSs were established. The presence of anti-dengue antibodies was determined in the paired samples. Results. DSS elution conditions were standardized as follows: 1 h at 4°C in 200 μl of DNase-, RNase-, and protease-free PBS (1x). The optimal volume of DSS eluate to be used in the IgG assay was 40 μl. Sensitivity of 94%, specificity of 93.3%, and kappa concordance of 0.87 were obtained when comparing the antidengue reactivity between DSSs and serum samples. Conclusion. DSS samples are useful for detecting anti-dengue IgG antibodies in the field.
AB - Background. Dried blood and serum samples are useful resources for detecting antiviral antibodies. The conditions for elution of the sample need to be optimized for each disease. Dengue is a widespread disease in Mexico which requires continuous surveillance. In this study, we standardized and validated a protocol for the specific detection of dengue antibodies from dried serum spots (DSSs). Methods. Paired serum and DSS samples from 66 suspected cases of dengue were collected in a clinic in Veracruz, Mexico. Samples were sent to our laboratory, where the conditions for optimal elution of DSSs were established. The presence of anti-dengue antibodies was determined in the paired samples. Results. DSS elution conditions were standardized as follows: 1 h at 4°C in 200 μl of DNase-, RNase-, and protease-free PBS (1x). The optimal volume of DSS eluate to be used in the IgG assay was 40 μl. Sensitivity of 94%, specificity of 93.3%, and kappa concordance of 0.87 were obtained when comparing the antidengue reactivity between DSSs and serum samples. Conclusion. DSS samples are useful for detecting anti-dengue IgG antibodies in the field.
UR - http://www.scopus.com/inward/record.url?scp=85021677462&partnerID=8YFLogxK
U2 - 10.1155/2017/7215259
DO - 10.1155/2017/7215259
M3 - Artículo
C2 - 28630868
SN - 2314-6133
VL - 2017
JO - BioMed Research International
JF - BioMed Research International
M1 - 7215259
ER -