Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production

Jose A. Pérez-Pimienta, Alejandra Vargas-Tah, Karla M. López-Ortega, Yessenia N. Medina-López, Jorge A. Mendoza-Pérez, Sayeny Avila, Seema Singh, Blake A. Simmons, Inés Loaces, Alfredo Martinez

Research output: Contribution to journalArticle

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Abstract

© 2016 Elsevier Ltd Agave bagasse (AGB) has gained recognition as a drought-tolerant biofuel feedstock with high productivity in semiarid regions. A comparative analysis of ionic liquid (IL) and organosolv (OV) pretreatment technologies in AGB was performed using a sequential enzymatic saccharification and fermentation (SESF) strategy with cellulolytic enzymes and the ethanologenic Escherichia coli strain MS04. After pretreatment, 86% of xylan and 45% of lignin were removed from OV-AGB, whereas IL-AGB reduced lignin content by 28% and xylan by 50% when compared to the untreated biomass. High glucan (>90%) and xylan (>83%) conversion was obtained with both pretreated samples. During the fermentation stage (48 h), 12.1 and 12.7 kg of ethanol were produced per 100 kg of untreated AGB for IL and OV, respectively. These comparative analyses showed the advantages of SESF using IL and OV in a biorefinery configuration where a better understanding of AGB recalcitrance is key for future applications.
Original languageAmerican English
Pages (from-to)191-198
Number of pages171
JournalBioresource Technology
DOIs
StatePublished - 1 Feb 2017

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Ionic Liquids
Saccharification
Bagasse
Ionic liquids
Fermentation
fermentation
ethanol
Ethanol
Xylans
lignin
Lignin
semiarid region
biofuel
Glucans
Biofuels
Drought
drought
enzyme
productivity
Feedstocks

Cite this

Pérez-Pimienta, Jose A. ; Vargas-Tah, Alejandra ; López-Ortega, Karla M. ; Medina-López, Yessenia N. ; Mendoza-Pérez, Jorge A. ; Avila, Sayeny ; Singh, Seema ; Simmons, Blake A. ; Loaces, Inés ; Martinez, Alfredo. / Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production. In: Bioresource Technology. 2017 ; pp. 191-198.
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Pérez-Pimienta, JA, Vargas-Tah, A, López-Ortega, KM, Medina-López, YN, Mendoza-Pérez, JA, Avila, S, Singh, S, Simmons, BA, Loaces, I & Martinez, A 2017, 'Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production', Bioresource Technology, pp. 191-198. https://doi.org/10.1016/j.biortech.2016.11.064

Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production. / Pérez-Pimienta, Jose A.; Vargas-Tah, Alejandra; López-Ortega, Karla M.; Medina-López, Yessenia N.; Mendoza-Pérez, Jorge A.; Avila, Sayeny; Singh, Seema; Simmons, Blake A.; Loaces, Inés; Martinez, Alfredo.

In: Bioresource Technology, 01.02.2017, p. 191-198.

Research output: Contribution to journalArticle

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AU - Mendoza-Pérez, Jorge A.

AU - Avila, Sayeny

AU - Singh, Seema

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AU - Loaces, Inés

AU - Martinez, Alfredo

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