Selective decrease of Na+/K+-ATPase activity in the brain of hypothyroid rats

Jorge Pacheco-Rosado, G. Arias-Citalán, R. Ortiz-Butrón, L. Rodríquez-Páez

Research output: Contribution to conferencePaperResearch

13 Citations (Scopus)

Abstract

The present work was performed in order to know if mild hypothyroidism in rats modifies the activity of the Na+/K+-ATPase in different regions of the brain. Male Wistar rats (300-350 g) were randomly divided into three groups: 1) control group (n=8) drank tap water. 2) hypothyroid group (n=8) treated with 60 mg/kg of methimazole in drinking water; and 3) replaced group (n=8) treated with 60 mg/kg of methimazole plus 35 μg/kg of thyroid hormone (T3) in drinking water. After four weeks of treatment, the rats of all groups were sacrificed by decapitation. The cortex, amygdala, hippocampus and cerebellum were dissected and frozen at -70°C until assay. For enzymatic assay, the tissues were homogenized. The Na+/K+-ATPase activity was determined by quantifying inorganic phosphate after the samples were incubated with ATP in the presence and absence of 1 mM ouabain. The Na+/K+-ATPase activity is expressed as μmoles Pi/hr/mg protein. The results showed that the Na +/K+-ATPase activity in the cortex, amygdala and hippocampus, but not in cerebellum, was lower in hypothyroid group than in control group (p<0.05). The co-administration of methimazole and T 3 avoided the decrease of Na+/K+-ATPase activity, except in amygdala. According to the results obtained we concluded that methimazole treatment decreased the Na+/K+-ATPase activity in the brain's regions which are related to seizures onset. That decrement in enzyme activity was avoided with the co-administration of thyroid hormone.
Original languageAmerican English
Pages52-54
Number of pages46
StatePublished - 1 Dec 2005
EventProceedings of the Western Pharmacology Society -
Duration: 1 Dec 2005 → …

Conference

ConferenceProceedings of the Western Pharmacology Society
Period1/12/05 → …

Fingerprint

hormone
brain
Rats
Brain
drinking water
assay
phosphate (inorganic)
enzyme activity
Potable water
protein
Assays
Adenosinetriphosphate
Enzyme activity
water
Adenosine Triphosphatases
Phosphates
Tissue
Proteins
tissue
seizure

Cite this

Pacheco-Rosado, J., Arias-Citalán, G., Ortiz-Butrón, R., & Rodríquez-Páez, L. (2005). Selective decrease of Na+/K+-ATPase activity in the brain of hypothyroid rats. 52-54. Paper presented at Proceedings of the Western Pharmacology Society, .
Pacheco-Rosado, Jorge ; Arias-Citalán, G. ; Ortiz-Butrón, R. ; Rodríquez-Páez, L. / Selective decrease of Na+/K+-ATPase activity in the brain of hypothyroid rats. Paper presented at Proceedings of the Western Pharmacology Society, .46 p.
@conference{fbd631a8f62c4efabb340486c6825959,
title = "Selective decrease of Na+/K+-ATPase activity in the brain of hypothyroid rats",
abstract = "The present work was performed in order to know if mild hypothyroidism in rats modifies the activity of the Na+/K+-ATPase in different regions of the brain. Male Wistar rats (300-350 g) were randomly divided into three groups: 1) control group (n=8) drank tap water. 2) hypothyroid group (n=8) treated with 60 mg/kg of methimazole in drinking water; and 3) replaced group (n=8) treated with 60 mg/kg of methimazole plus 35 μg/kg of thyroid hormone (T3) in drinking water. After four weeks of treatment, the rats of all groups were sacrificed by decapitation. The cortex, amygdala, hippocampus and cerebellum were dissected and frozen at -70°C until assay. For enzymatic assay, the tissues were homogenized. The Na+/K+-ATPase activity was determined by quantifying inorganic phosphate after the samples were incubated with ATP in the presence and absence of 1 mM ouabain. The Na+/K+-ATPase activity is expressed as μmoles Pi/hr/mg protein. The results showed that the Na +/K+-ATPase activity in the cortex, amygdala and hippocampus, but not in cerebellum, was lower in hypothyroid group than in control group (p<0.05). The co-administration of methimazole and T 3 avoided the decrease of Na+/K+-ATPase activity, except in amygdala. According to the results obtained we concluded that methimazole treatment decreased the Na+/K+-ATPase activity in the brain's regions which are related to seizures onset. That decrement in enzyme activity was avoided with the co-administration of thyroid hormone.",
author = "Jorge Pacheco-Rosado and G. Arias-Cital{\'a}n and R. Ortiz-Butr{\'o}n and L. Rodr{\'i}quez-P{\'a}ez",
year = "2005",
month = "12",
day = "1",
language = "American English",
pages = "52--54",
note = "Proceedings of the Western Pharmacology Society ; Conference date: 01-12-2005",

}

Pacheco-Rosado, J, Arias-Citalán, G, Ortiz-Butrón, R & Rodríquez-Páez, L 2005, 'Selective decrease of Na+/K+-ATPase activity in the brain of hypothyroid rats' Paper presented at Proceedings of the Western Pharmacology Society, 1/12/05, pp. 52-54.

Selective decrease of Na+/K+-ATPase activity in the brain of hypothyroid rats. / Pacheco-Rosado, Jorge; Arias-Citalán, G.; Ortiz-Butrón, R.; Rodríquez-Páez, L.

2005. 52-54 Paper presented at Proceedings of the Western Pharmacology Society, .

Research output: Contribution to conferencePaperResearch

TY - CONF

T1 - Selective decrease of Na+/K+-ATPase activity in the brain of hypothyroid rats

AU - Pacheco-Rosado, Jorge

AU - Arias-Citalán, G.

AU - Ortiz-Butrón, R.

AU - Rodríquez-Páez, L.

PY - 2005/12/1

Y1 - 2005/12/1

N2 - The present work was performed in order to know if mild hypothyroidism in rats modifies the activity of the Na+/K+-ATPase in different regions of the brain. Male Wistar rats (300-350 g) were randomly divided into three groups: 1) control group (n=8) drank tap water. 2) hypothyroid group (n=8) treated with 60 mg/kg of methimazole in drinking water; and 3) replaced group (n=8) treated with 60 mg/kg of methimazole plus 35 μg/kg of thyroid hormone (T3) in drinking water. After four weeks of treatment, the rats of all groups were sacrificed by decapitation. The cortex, amygdala, hippocampus and cerebellum were dissected and frozen at -70°C until assay. For enzymatic assay, the tissues were homogenized. The Na+/K+-ATPase activity was determined by quantifying inorganic phosphate after the samples were incubated with ATP in the presence and absence of 1 mM ouabain. The Na+/K+-ATPase activity is expressed as μmoles Pi/hr/mg protein. The results showed that the Na +/K+-ATPase activity in the cortex, amygdala and hippocampus, but not in cerebellum, was lower in hypothyroid group than in control group (p<0.05). The co-administration of methimazole and T 3 avoided the decrease of Na+/K+-ATPase activity, except in amygdala. According to the results obtained we concluded that methimazole treatment decreased the Na+/K+-ATPase activity in the brain's regions which are related to seizures onset. That decrement in enzyme activity was avoided with the co-administration of thyroid hormone.

AB - The present work was performed in order to know if mild hypothyroidism in rats modifies the activity of the Na+/K+-ATPase in different regions of the brain. Male Wistar rats (300-350 g) were randomly divided into three groups: 1) control group (n=8) drank tap water. 2) hypothyroid group (n=8) treated with 60 mg/kg of methimazole in drinking water; and 3) replaced group (n=8) treated with 60 mg/kg of methimazole plus 35 μg/kg of thyroid hormone (T3) in drinking water. After four weeks of treatment, the rats of all groups were sacrificed by decapitation. The cortex, amygdala, hippocampus and cerebellum were dissected and frozen at -70°C until assay. For enzymatic assay, the tissues were homogenized. The Na+/K+-ATPase activity was determined by quantifying inorganic phosphate after the samples were incubated with ATP in the presence and absence of 1 mM ouabain. The Na+/K+-ATPase activity is expressed as μmoles Pi/hr/mg protein. The results showed that the Na +/K+-ATPase activity in the cortex, amygdala and hippocampus, but not in cerebellum, was lower in hypothyroid group than in control group (p<0.05). The co-administration of methimazole and T 3 avoided the decrease of Na+/K+-ATPase activity, except in amygdala. According to the results obtained we concluded that methimazole treatment decreased the Na+/K+-ATPase activity in the brain's regions which are related to seizures onset. That decrement in enzyme activity was avoided with the co-administration of thyroid hormone.

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=30144438893&origin=inward

UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=30144438893&origin=inward

M3 - Paper

SP - 52

EP - 54

ER -

Pacheco-Rosado J, Arias-Citalán G, Ortiz-Butrón R, Rodríquez-Páez L. Selective decrease of Na+/K+-ATPase activity in the brain of hypothyroid rats. 2005. Paper presented at Proceedings of the Western Pharmacology Society, .