Role of FcγRIII in the nasal cavity of BALB/c mice in the primary amebic meningoencephalitis protection model

Diego Alexander Rojas-Ortega, Saúl Rojas-Hernández, María Elena Sánchez-Mendoza, Modesto Gómez-López, Jennifer Viridiana Sánchez-Camacho, Erika Rosales-Cruz, María Maricela Carrasco Yépez

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Different mechanisms of the host immune response against the primary amebic meningoencephalitis (PAM) in the mouse protection model have been described. It has been proposed that antibodies opsonize Naegleria fowleri trophozoites; subsequently, the polymorphonuclear cells (PMNs) surround the trophozoites to avoid the infection. FcγRs activate signaling pathways of adapter proteins such as Syk and Hck on PMNs to promote different effector cell functions which are induced by the Fc portion of the antibody-antigen complexes. In this work, we analyzed the activation of PMNs, epithelial cells, and nasal passage cells via the expression of Syk and Hck genes. Our results showed an increment of the FcγRIII and IgG subclasses in the nasal cavity from immunized mice as well as Syk and Hck expression was increased, whereas in the in vitro assay, we observed that when the trophozoites of N. fowleri were opsonized with IgG anti-N. fowleri and interacted with PMN, the expression of Syk and Hck was also increased. We suggest that PMNs are activated via their FcγRIII, which leads to the elimination of the trophozoites in vitro, while in the nasal cavity, the adhesion and consequently infection are avoided.

Original languageEnglish
Pages (from-to)1087-1105
Number of pages19
JournalParasitology Research
Volume122
Issue number5
DOIs
StatePublished - May 2023

Keywords

  • FcγRIII
  • Hck
  • IgG
  • Naegleria fowleri
  • Neutrophils
  • Syk

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