TY - JOUR
T1 - Role of FcγRIII in the nasal cavity of BALB/c mice in the primary amebic meningoencephalitis protection model
AU - Rojas-Ortega, Diego Alexander
AU - Rojas-Hernández, Saúl
AU - Sánchez-Mendoza, María Elena
AU - Gómez-López, Modesto
AU - Sánchez-Camacho, Jennifer Viridiana
AU - Rosales-Cruz, Erika
AU - Yépez, María Maricela Carrasco
N1 - Publisher Copyright:
© 2023, The Author(s).
PY - 2023/5
Y1 - 2023/5
N2 - Different mechanisms of the host immune response against the primary amebic meningoencephalitis (PAM) in the mouse protection model have been described. It has been proposed that antibodies opsonize Naegleria fowleri trophozoites; subsequently, the polymorphonuclear cells (PMNs) surround the trophozoites to avoid the infection. FcγRs activate signaling pathways of adapter proteins such as Syk and Hck on PMNs to promote different effector cell functions which are induced by the Fc portion of the antibody-antigen complexes. In this work, we analyzed the activation of PMNs, epithelial cells, and nasal passage cells via the expression of Syk and Hck genes. Our results showed an increment of the FcγRIII and IgG subclasses in the nasal cavity from immunized mice as well as Syk and Hck expression was increased, whereas in the in vitro assay, we observed that when the trophozoites of N. fowleri were opsonized with IgG anti-N. fowleri and interacted with PMN, the expression of Syk and Hck was also increased. We suggest that PMNs are activated via their FcγRIII, which leads to the elimination of the trophozoites in vitro, while in the nasal cavity, the adhesion and consequently infection are avoided.
AB - Different mechanisms of the host immune response against the primary amebic meningoencephalitis (PAM) in the mouse protection model have been described. It has been proposed that antibodies opsonize Naegleria fowleri trophozoites; subsequently, the polymorphonuclear cells (PMNs) surround the trophozoites to avoid the infection. FcγRs activate signaling pathways of adapter proteins such as Syk and Hck on PMNs to promote different effector cell functions which are induced by the Fc portion of the antibody-antigen complexes. In this work, we analyzed the activation of PMNs, epithelial cells, and nasal passage cells via the expression of Syk and Hck genes. Our results showed an increment of the FcγRIII and IgG subclasses in the nasal cavity from immunized mice as well as Syk and Hck expression was increased, whereas in the in vitro assay, we observed that when the trophozoites of N. fowleri were opsonized with IgG anti-N. fowleri and interacted with PMN, the expression of Syk and Hck was also increased. We suggest that PMNs are activated via their FcγRIII, which leads to the elimination of the trophozoites in vitro, while in the nasal cavity, the adhesion and consequently infection are avoided.
KW - FcγRIII
KW - Hck
KW - IgG
KW - Naegleria fowleri
KW - Neutrophils
KW - Syk
UR - http://www.scopus.com/inward/record.url?scp=85149890195&partnerID=8YFLogxK
U2 - 10.1007/s00436-023-07810-w
DO - 10.1007/s00436-023-07810-w
M3 - Artículo
C2 - 36913025
AN - SCOPUS:85149890195
SN - 0932-0113
VL - 122
SP - 1087
EP - 1105
JO - Parasitology Research
JF - Parasitology Research
IS - 5
ER -