TY - JOUR
T1 - Response to osmotic stress and temperature of the fungus Ustilago maydis
AU - Salmerón-Santiago, Karina Gabriela
AU - Pardo, Juan Pablo
AU - Flores-Herrera, Oscar
AU - Mendoza-Hernández, Guillermo
AU - Miranda-Arango, Manuel
AU - Guerra-Sánchez, Guadalupe
N1 - Funding Information:
Acknowledgments This work was supported in part by grants from CGPI-IPN (20070822, 20080561, and 2009656), from Consejo Nac-ional de Ciencia y Tecnología (CONACyT 59855), and from Prog-rama de Apoyo a Proyectos de Investigación e Innovación Tecnológica (PAPIIT IN219107-3), Universidad Nacional Autónoma de México. Karina Salmerón Santiago received doctoral fellowship Grants from CONACyT (2007–2010) and PIFI-IPN (2007–2009).
PY - 2011/10
Y1 - 2011/10
N2 - Ustilago maydis is a fungal pathogen which is exposed during its life cycle to both abiotic and biotic stresses before and after the infection of maize. To cope with extreme environmental changes, microorganisms usually accumulate the disaccharide trehalose. We have investigated both the accumulation of trehalose and the activity of trehalase during the adaptation of U. maydis haploid cells to thermal, sorbitol, and NaCl stresses. Sorbitol and sodium chloride induced sustained accumulation of trehalose, while a transient increase was observed under heat stress. Sorbitol stressed cells showed higher trehalase activity compared with control cells and to those stressed by NaCl and high temperature. Addition of cycloheximide, a protein synthesis inhibitor, did not affect the trehalose accumulation during the first 15 min, but basal levels of trehalose were reached after the second period of 15 min. The proteomic analysis of the response of U. maydis to temperature, sorbitol, and salt stresses indicated a complex pattern which highlights the change of 18 proteins involved in carbohydrate and amino acid metabolism, protein folding, redox regulation, ion homeostasis, and stress response. We hypothesize that trehalose accumulation during sorbitol stress in U. maydis might be related to the adaptation of this organism during plant infection.
AB - Ustilago maydis is a fungal pathogen which is exposed during its life cycle to both abiotic and biotic stresses before and after the infection of maize. To cope with extreme environmental changes, microorganisms usually accumulate the disaccharide trehalose. We have investigated both the accumulation of trehalose and the activity of trehalase during the adaptation of U. maydis haploid cells to thermal, sorbitol, and NaCl stresses. Sorbitol and sodium chloride induced sustained accumulation of trehalose, while a transient increase was observed under heat stress. Sorbitol stressed cells showed higher trehalase activity compared with control cells and to those stressed by NaCl and high temperature. Addition of cycloheximide, a protein synthesis inhibitor, did not affect the trehalose accumulation during the first 15 min, but basal levels of trehalose were reached after the second period of 15 min. The proteomic analysis of the response of U. maydis to temperature, sorbitol, and salt stresses indicated a complex pattern which highlights the change of 18 proteins involved in carbohydrate and amino acid metabolism, protein folding, redox regulation, ion homeostasis, and stress response. We hypothesize that trehalose accumulation during sorbitol stress in U. maydis might be related to the adaptation of this organism during plant infection.
KW - Heat stress
KW - Osmotic stress
KW - Trehalose accumulation
KW - Ustilago maydis
KW - Yeast trehalase
UR - http://www.scopus.com/inward/record.url?scp=82455188106&partnerID=8YFLogxK
U2 - 10.1007/s00203-011-0706-9
DO - 10.1007/s00203-011-0706-9
M3 - Artículo de revisión
SN - 0302-8933
VL - 193
SP - 701
EP - 709
JO - Archives of Microbiology
JF - Archives of Microbiology
IS - 10
ER -