TY - JOUR
T1 - Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24
AU - Li, Chune
AU - Yang, Jinshui
AU - Wang, Xin
AU - Wang, Entao
AU - Li, Baozhen
AU - He, Ruoxue
AU - Yuan, Hongli
N1 - Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Phosphate accumulating bacterium Pseudomonas stutzeri YG-24 exhibited efficient heterotrophic nitrification and aerobic denitrification ability. Single factor experiments showed that both heterotrophic nitrification and aerobic denitrification occurred with sodium citrate as carbon source and lower C/N ratio of 8. High average NH4+-N, NO2--N and NO3--N removal rates of 8.75, 7.51 and 7.73mgL-1h-1 were achieved. The application of strain YG-24 in wastewater samples resulted in TN, NH4+-N, NO2--N, NO3--N and P removal efficiencies of 85.28%, 88.13%, 86.15%, 70.83% and 51.21%. Sequencing and quantitative amplification by real-time PCR of napA, nirS and ppk showed that nitrogen removal pathway of strain YG-24 was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification (NH4+-N to NO2--N and then to gaseous nitrogen) directly. These results demonstrated the strain as a suitable candidate to simultaneously remove both nitrogen and phosphate in wastewater treatment.
AB - Phosphate accumulating bacterium Pseudomonas stutzeri YG-24 exhibited efficient heterotrophic nitrification and aerobic denitrification ability. Single factor experiments showed that both heterotrophic nitrification and aerobic denitrification occurred with sodium citrate as carbon source and lower C/N ratio of 8. High average NH4+-N, NO2--N and NO3--N removal rates of 8.75, 7.51 and 7.73mgL-1h-1 were achieved. The application of strain YG-24 in wastewater samples resulted in TN, NH4+-N, NO2--N, NO3--N and P removal efficiencies of 85.28%, 88.13%, 86.15%, 70.83% and 51.21%. Sequencing and quantitative amplification by real-time PCR of napA, nirS and ppk showed that nitrogen removal pathway of strain YG-24 was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification (NH4+-N to NO2--N and then to gaseous nitrogen) directly. These results demonstrated the strain as a suitable candidate to simultaneously remove both nitrogen and phosphate in wastewater treatment.
KW - Aerobic denitrification
KW - Heterotrophic nitrification
KW - Nitrogen removal
KW - Phosphate accumulating bacterium
KW - Pseudomonas stutzeri YG-24
UR - http://www.scopus.com/inward/record.url?scp=84922379008&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2015.01.100
DO - 10.1016/j.biortech.2015.01.100
M3 - Artículo
SN - 0960-8524
VL - 182
SP - 18
EP - 25
JO - Bioresource Technology
JF - Bioresource Technology
ER -