Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24

Chune Li, Jinshui Yang, Xin Wang, Entao Wang, Baozhen Li, Ruoxue He, Hongli Yuan

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    Abstract

    © 2015 Elsevier Ltd. Phosphate accumulating bacterium Pseudomonas stutzeri YG-24 exhibited efficient heterotrophic nitrification and aerobic denitrification ability. Single factor experiments showed that both heterotrophic nitrification and aerobic denitrification occurred with sodium citrate as carbon source and lower C/N ratio of 8. High average NH4+-N, NO2--N and NO3--N removal rates of 8.75, 7.51 and 7.73mgL-1h-1 were achieved. The application of strain YG-24 in wastewater samples resulted in TN, NH4+-N, NO2--N, NO3--N and P removal efficiencies of 85.28%, 88.13%, 86.15%, 70.83% and 51.21%. Sequencing and quantitative amplification by real-time PCR of napA, nirS and ppk showed that nitrogen removal pathway of strain YG-24 was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification (NH4+-N to NO2--N and then to gaseous nitrogen) directly. These results demonstrated the strain as a suitable candidate to simultaneously remove both nitrogen and phosphate in wastewater treatment.
    Original languageAmerican English
    Pages (from-to)18-25
    Number of pages15
    JournalBioresource Technology
    DOIs
    StatePublished - 1 Apr 2015

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