TY - GEN
T1 - Raman spectrum modification of CdSe/ZnS quantum dots at the bio-conjugation to IgG antibodies
AU - Diaz Cano, A. I.
AU - Douda, J.
AU - Gonzalez Vargas, C. R.
AU - Gazarian, K.
PY - 2012
Y1 - 2012
N2 - The paper presents the analysis of Raman scattering spectra of non-conjugated and bio-conjugated CdSe/ZnS core-shell quantum dots in the range of Raman shifts of 80-2000 cm -1. Commercial CdSe/ZnS QDs covered by polymer and characterized by color emission with the maxima at 605 and 655 nm (1.89 and 2.04 eV) were used. Raman scattering spectra were measured at 300K and the excitation by the line 785.0 nm of a solid state LED. The analysis of Raman spectra has shown that the QD bio-conjugation to the immunoglobulin G (IgG) antibodies of the Pseudorabies virus is accompanied by the changes of the intensity of Raman lines related to the CdSe/ZnS core/shell QDs, PEG polymer covered QDs, the Si substrate and/or some organic groups of antibody molecules. The comparison of Raman spectra of CdSe/ZnS QDs with different sizes in non-conjugated and bio-conjugated states gives the opportunity to detect the bio-conjugation without mistake.
AB - The paper presents the analysis of Raman scattering spectra of non-conjugated and bio-conjugated CdSe/ZnS core-shell quantum dots in the range of Raman shifts of 80-2000 cm -1. Commercial CdSe/ZnS QDs covered by polymer and characterized by color emission with the maxima at 605 and 655 nm (1.89 and 2.04 eV) were used. Raman scattering spectra were measured at 300K and the excitation by the line 785.0 nm of a solid state LED. The analysis of Raman spectra has shown that the QD bio-conjugation to the immunoglobulin G (IgG) antibodies of the Pseudorabies virus is accompanied by the changes of the intensity of Raman lines related to the CdSe/ZnS core/shell QDs, PEG polymer covered QDs, the Si substrate and/or some organic groups of antibody molecules. The comparison of Raman spectra of CdSe/ZnS QDs with different sizes in non-conjugated and bio-conjugated states gives the opportunity to detect the bio-conjugation without mistake.
UR - http://www.scopus.com/inward/record.url?scp=84861163744&partnerID=8YFLogxK
U2 - 10.1557/opl.2012.161
DO - 10.1557/opl.2012.161
M3 - Contribución a la conferencia
AN - SCOPUS:84861163744
SN - 9781605113487
T3 - Materials Research Society Symposium Proceedings
SP - 45
EP - 50
BT - Nanostructured Materials and Nanotechnology
T2 - 20th International Materials Research Congress, IMRC 2011
Y2 - 14 August 2011 through 19 August 2011
ER -