Quantification of vitellin/vitellogenin-like proteins in the oyster Crassostrea corteziensis (Hertlein 1951) as a tool to predict the degree of gonad maturity

Fabiola G. Arcos, Ana María Ibarra, María Del Carmen Rodríguez-Jaramillo, Ethel Awilda García-Latorre, Celia Vazquez-Boucard

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The oyster's reproductive process is poorly documented, especially in terms of a quantitative approach. In recent years, investigations with this species have been directed at determining important reproductive factors. Within this scope, techniques that allow standardized and accurate quantitative estimations of gonad development have become of primary importance. In this study, the histological characteristics and the levels of vitellin/vitellogenin-like proteins (Vn/Vtg) from ovaries of the Mexican Pacific 'pleasure' oyster Crassostrea corteziensis (Hertlein 1951) were analysed during different stages of gonad maturation using quantitative histological techniques and an enzyme-linked immunosorbent assay. This was performed in order to evaluate a possible quantitative tool to predict the degrees of gonad maturity and to analyse the biological implications of the findings relative not only to broodstock conditioning but also to natural populations. Using this information, we expect to be able to undertake further research on different reproductive aspects of this oyster species, including, among others, evaluation of the response in Vn/Vtg concentrations to different diets and environmental conditions during laboratory conditioning. © 2009 Blackwell Publishing Ltd.
Original languageAmerican English
Pages (from-to)644-655
Number of pages578
JournalAquaculture Research
DOIs
StatePublished - 1 Apr 2009

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Crassostrea corteziensis
vitellin
vitellogenin
conditioning
oysters
gonads
protein
maturation
breeding stock
proteins
Crassostrea gigas
environmental conditions
assay
enzyme
diet
quantitative analysis
enzyme-linked immunosorbent assay
environmental factors

Cite this

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title = "Quantification of vitellin/vitellogenin-like proteins in the oyster Crassostrea corteziensis (Hertlein 1951) as a tool to predict the degree of gonad maturity",
abstract = "The oyster's reproductive process is poorly documented, especially in terms of a quantitative approach. In recent years, investigations with this species have been directed at determining important reproductive factors. Within this scope, techniques that allow standardized and accurate quantitative estimations of gonad development have become of primary importance. In this study, the histological characteristics and the levels of vitellin/vitellogenin-like proteins (Vn/Vtg) from ovaries of the Mexican Pacific 'pleasure' oyster Crassostrea corteziensis (Hertlein 1951) were analysed during different stages of gonad maturation using quantitative histological techniques and an enzyme-linked immunosorbent assay. This was performed in order to evaluate a possible quantitative tool to predict the degrees of gonad maturity and to analyse the biological implications of the findings relative not only to broodstock conditioning but also to natural populations. Using this information, we expect to be able to undertake further research on different reproductive aspects of this oyster species, including, among others, evaluation of the response in Vn/Vtg concentrations to different diets and environmental conditions during laboratory conditioning. {\circledC} 2009 Blackwell Publishing Ltd.",
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Quantification of vitellin/vitellogenin-like proteins in the oyster Crassostrea corteziensis (Hertlein 1951) as a tool to predict the degree of gonad maturity. / Arcos, Fabiola G.; Ibarra, Ana María; Rodríguez-Jaramillo, María Del Carmen; García-Latorre, Ethel Awilda; Vazquez-Boucard, Celia.

In: Aquaculture Research, 01.04.2009, p. 644-655.

Research output: Contribution to journalArticle

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