Purification of Gastroprotective Triterpenoids from the Stem Bark of Amphipterygium adstringens; Role of Prostaglandins, Sulfhydryls, Nitric Oxide and Capsaicin-Sensitive Neurons

Jesús Arrieta, Javier Benitez, Edith Flores, Carlos Castillo, Andrés Navarrete

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

In this investigation three bioactive compounds, responsible for the gastroprotective property of Amphipterygium adstringens, were purified from an active dichloromethane fraction. These compounds were 3α- hydroxymasticadienonic acid, β-sitosterol and 3-epi-oleanolic acid. The latter was the most active compound (88.8% of gastroprotection) followed by 3α-hydroxymasticadienoic acid and β-sitosterol (69.8 and 42.5% of gastroprotection, respectively). Carbenoxolone was used as positive control and it showed 88.4% of gastroprotection. Masticadienonic acid was also isolated from the active fraction, but it was unable to inhibit the ethanol-induced gastric lesions. The gastroprotection of the methanol extract was completely inhibited by the pretreatment with I-NAME and attenuated by pretreatment with indomethacin and N-ethylmaleimide. These results suggest that endogenous nitric oxide plays an important role in the gastroprotection of A. adstringens methanol extract on ethanol-induced gastric mucosal lesions and that there is partial participation by prostaglandins and endogenous sulfhydryls. The effect of 3α-hydroxymasticadienonic acid was attenuated only by pretreatment with N-ethylmaleimide, indicating that endogenous sulfhydryls (thiols) participate in its gastroprotective mechanism. Capsaicin-sensitive afferent neurons do not participate in the gastroprotection of either the methanol extract or 3α-hydroxymasticadienoic acid.
Original languageAmerican English
Pages (from-to)905-909
Number of pages814
JournalPlanta Medica
DOIs
StatePublished - 1 Oct 2003

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Capsaicin
Neurons
Prostaglandins
Purification
Nitric Oxide
Methanol
Ethylmaleimide
Acids
Stomach
Ethanol
Carbenoxolone
Oleanolic Acid
Afferent Neurons
Methylene Chloride
Sulfhydryl Compounds
Indomethacin
gamma-sitosterol

Cite this

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title = "Purification of Gastroprotective Triterpenoids from the Stem Bark of Amphipterygium adstringens; Role of Prostaglandins, Sulfhydryls, Nitric Oxide and Capsaicin-Sensitive Neurons",
abstract = "In this investigation three bioactive compounds, responsible for the gastroprotective property of Amphipterygium adstringens, were purified from an active dichloromethane fraction. These compounds were 3α- hydroxymasticadienonic acid, β-sitosterol and 3-epi-oleanolic acid. The latter was the most active compound (88.8{\%} of gastroprotection) followed by 3α-hydroxymasticadienoic acid and β-sitosterol (69.8 and 42.5{\%} of gastroprotection, respectively). Carbenoxolone was used as positive control and it showed 88.4{\%} of gastroprotection. Masticadienonic acid was also isolated from the active fraction, but it was unable to inhibit the ethanol-induced gastric lesions. The gastroprotection of the methanol extract was completely inhibited by the pretreatment with I-NAME and attenuated by pretreatment with indomethacin and N-ethylmaleimide. These results suggest that endogenous nitric oxide plays an important role in the gastroprotection of A. adstringens methanol extract on ethanol-induced gastric mucosal lesions and that there is partial participation by prostaglandins and endogenous sulfhydryls. The effect of 3α-hydroxymasticadienonic acid was attenuated only by pretreatment with N-ethylmaleimide, indicating that endogenous sulfhydryls (thiols) participate in its gastroprotective mechanism. Capsaicin-sensitive afferent neurons do not participate in the gastroprotection of either the methanol extract or 3α-hydroxymasticadienoic acid.",
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Purification of Gastroprotective Triterpenoids from the Stem Bark of Amphipterygium adstringens; Role of Prostaglandins, Sulfhydryls, Nitric Oxide and Capsaicin-Sensitive Neurons. / Arrieta, Jesús; Benitez, Javier; Flores, Edith; Castillo, Carlos; Navarrete, Andrés.

In: Planta Medica, 01.10.2003, p. 905-909.

Research output: Contribution to journalArticle

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T1 - Purification of Gastroprotective Triterpenoids from the Stem Bark of Amphipterygium adstringens; Role of Prostaglandins, Sulfhydryls, Nitric Oxide and Capsaicin-Sensitive Neurons

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