TY - JOUR
T1 - Protective effects of garlic powder against potassium dichromate-induced oxidative stress and nephrotoxicity
AU - Pedraza-Chaverri, José
AU - Yam-Canul, Paola
AU - Chirino, Yolanda I.
AU - Sánchez-González, Dolores Javier
AU - Martínez-Martínez, Claudia María
AU - Cruz, Cristino
AU - Medina-Campos, Omar N.
N1 - Funding Information:
This work was supported by CONACYT (Grant No. 40009M). Axiovert 200 M confocal microscope was donated by Fundación Gonzalo Rio Arronte IAP México. We thank Giovanna Merchand-Reyes for her technical support.
PY - 2008/2
Y1 - 2008/2
N2 - Potassium dichromate (K2Cr2O7)-induced nephrotoxicity is associated with oxidative stress. In the present work the effect of garlic powder, a recognized antioxidant, on K2Cr2O7-induced nephrotoxicity and oxidative stress was studied. Rats were fed a 2% garlic powder diet for 1 month. A single injection of K2Cr2O7 (15 mg/kg) to rats induced tubule interstitial damage and an increase in the following markers of renal injury 2 days later: blood urea nitrogen (4.6-fold), serum creatinine (9.7-fold), proteinuria (35.9-fold), urinary excretion of N-acetyl-β-d-glucosaminidase (12.9-fold) and glutathione-S-transferase (2.3-fold) and a decrease of 65% in serum glutathione peroxidase activity. In addition, K2Cr2O7 injection increased the following nitrosative and oxidative stress markers in kidney: 3-nitrotyrosine (1.9-fold), 4-hydroxy-2-nonenal (2.1-fold), malondialdehyde (1.8-fold) and protein carbonyl content (1.7-fold). It was found that garlic powder feeding was able to prevent by 44-71% the alterations in the markers of renal injury studied, by 55% the histological damage, and by 47-100% the increase in markers of oxidative and nitrosative stress. It is concluded that the ability of garlic powder to ameliorate K2Cr2O7-induced renal injury is associated with its antioxidant properties. Our data support the use of garlic powder as a renoprotective agent.
AB - Potassium dichromate (K2Cr2O7)-induced nephrotoxicity is associated with oxidative stress. In the present work the effect of garlic powder, a recognized antioxidant, on K2Cr2O7-induced nephrotoxicity and oxidative stress was studied. Rats were fed a 2% garlic powder diet for 1 month. A single injection of K2Cr2O7 (15 mg/kg) to rats induced tubule interstitial damage and an increase in the following markers of renal injury 2 days later: blood urea nitrogen (4.6-fold), serum creatinine (9.7-fold), proteinuria (35.9-fold), urinary excretion of N-acetyl-β-d-glucosaminidase (12.9-fold) and glutathione-S-transferase (2.3-fold) and a decrease of 65% in serum glutathione peroxidase activity. In addition, K2Cr2O7 injection increased the following nitrosative and oxidative stress markers in kidney: 3-nitrotyrosine (1.9-fold), 4-hydroxy-2-nonenal (2.1-fold), malondialdehyde (1.8-fold) and protein carbonyl content (1.7-fold). It was found that garlic powder feeding was able to prevent by 44-71% the alterations in the markers of renal injury studied, by 55% the histological damage, and by 47-100% the increase in markers of oxidative and nitrosative stress. It is concluded that the ability of garlic powder to ameliorate K2Cr2O7-induced renal injury is associated with its antioxidant properties. Our data support the use of garlic powder as a renoprotective agent.
KW - 3-Nitrotyrosine
KW - 4-Hydroxy-2-nonenal
KW - Antioxidant
KW - Garlic
KW - Malondialdehyde
KW - Nephrotoxicity
KW - Oxidative stress
KW - Potassium dichromate
KW - Protein carbonyl content
UR - http://www.scopus.com/inward/record.url?scp=37449002611&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2007.09.088
DO - 10.1016/j.fct.2007.09.088
M3 - Artículo
C2 - 17950512
SN - 0278-6915
VL - 46
SP - 619
EP - 627
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
IS - 2
ER -