(Pro)renin/renin receptor expression during normal and preeclamptic pregnancy in rats

M. A. Avila-Ramírez, R. L. Esteban-Martínez, E. López-Moctezuma, L. Anguiano-Robledo, M. E. Hernández-Campos, P. López-Sánchez

Research output: Contribution to journalArticleResearchpeer-review

Abstract

© 2018 Elsevier Inc. Aims: Pregnancy is a physiological stage with profound cardiovascular changes leading to hypotension. Preeclampsia (PE) reverts these normal changes inducing hypertension. Renin-angiotensin system (RAS) has been related in PE genesis. It has been reported a novel receptor in the system, the Prorenin/Renin receptor (PRR), with several roles in renal and cardiovascular illnesses. It is not known, however, if PRR changes its expression or is activated during normal or PE-complicated pregnancy on tissues intimately related to hypertension. So, the aim of this work was to describe PRR expression during normal and hypertensive pregnancy in rats. Methods: We used a subrenal aortic coarctation (SRAC) model in rats. Atria, septum and ventricular heart tissue, aorta and renal tissue samples were homogenized and immunoblotted using anti-PRR and anti-PLZF antibodies. We also measured gene expression by RT-PCR. Key findings: Hypertension and proteinuria were observed in SRAC-pregnant rats. In pregnant, non-SRAC rats, PRR showed a higher expression of both, gene and protein compared to non-pregnant rats in heart, aorta and kidney tissues. PE induces a very high expression of PRR in cardiac tissues and, on the contrary, decreases PRR expression in both, aorta and kidney. PLZF, a marker of PRR function, was augmented only in aorta and kidney in non-SRAC pregnant rats. In SRAC-pregnant rats, PLZF increment disappeared. Significance: These findings indicate that PRR expression changes differently during pregnancy and PE in tissues related to cardiovascular functions and suggest a probable participation of the receptor during normal and preeclamptic pregnancy in the rat.
Original languageAmerican English
Pages (from-to)22-28
Number of pages19
JournalLife Sciences
DOIs
StatePublished - 1 Jan 2019

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Renin
Rats
Aortic Coarctation
Pregnancy
Pre-Eclampsia
Aorta
Kidney
Hypertension
Tissue
Heart Septum
prorenin receptor
Ventricular Septum
Renin-Angiotensin System
Proteinuria
Hypotension
Anti-Idiotypic Antibodies
Gene Expression
Polymerase Chain Reaction
Angiotensins
Gene expression

Cite this

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title = "(Pro)renin/renin receptor expression during normal and preeclamptic pregnancy in rats",
abstract = "{\circledC} 2018 Elsevier Inc. Aims: Pregnancy is a physiological stage with profound cardiovascular changes leading to hypotension. Preeclampsia (PE) reverts these normal changes inducing hypertension. Renin-angiotensin system (RAS) has been related in PE genesis. It has been reported a novel receptor in the system, the Prorenin/Renin receptor (PRR), with several roles in renal and cardiovascular illnesses. It is not known, however, if PRR changes its expression or is activated during normal or PE-complicated pregnancy on tissues intimately related to hypertension. So, the aim of this work was to describe PRR expression during normal and hypertensive pregnancy in rats. Methods: We used a subrenal aortic coarctation (SRAC) model in rats. Atria, septum and ventricular heart tissue, aorta and renal tissue samples were homogenized and immunoblotted using anti-PRR and anti-PLZF antibodies. We also measured gene expression by RT-PCR. Key findings: Hypertension and proteinuria were observed in SRAC-pregnant rats. In pregnant, non-SRAC rats, PRR showed a higher expression of both, gene and protein compared to non-pregnant rats in heart, aorta and kidney tissues. PE induces a very high expression of PRR in cardiac tissues and, on the contrary, decreases PRR expression in both, aorta and kidney. PLZF, a marker of PRR function, was augmented only in aorta and kidney in non-SRAC pregnant rats. In SRAC-pregnant rats, PLZF increment disappeared. Significance: These findings indicate that PRR expression changes differently during pregnancy and PE in tissues related to cardiovascular functions and suggest a probable participation of the receptor during normal and preeclamptic pregnancy in the rat.",
author = "Avila-Ram{\'i}rez, {M. A.} and Esteban-Mart{\'i}nez, {R. L.} and E. L{\'o}pez-Moctezuma and L. Anguiano-Robledo and Hern{\'a}ndez-Campos, {M. E.} and P. L{\'o}pez-S{\'a}nchez",
year = "2019",
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doi = "10.1016/j.lfs.2018.11.017",
language = "American English",
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(Pro)renin/renin receptor expression during normal and preeclamptic pregnancy in rats. / Avila-Ramírez, M. A.; Esteban-Martínez, R. L.; López-Moctezuma, E.; Anguiano-Robledo, L.; Hernández-Campos, M. E.; López-Sánchez, P.

In: Life Sciences, 01.01.2019, p. 22-28.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - (Pro)renin/renin receptor expression during normal and preeclamptic pregnancy in rats

AU - Avila-Ramírez, M. A.

AU - Esteban-Martínez, R. L.

AU - López-Moctezuma, E.

AU - Anguiano-Robledo, L.

AU - Hernández-Campos, M. E.

AU - López-Sánchez, P.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - © 2018 Elsevier Inc. Aims: Pregnancy is a physiological stage with profound cardiovascular changes leading to hypotension. Preeclampsia (PE) reverts these normal changes inducing hypertension. Renin-angiotensin system (RAS) has been related in PE genesis. It has been reported a novel receptor in the system, the Prorenin/Renin receptor (PRR), with several roles in renal and cardiovascular illnesses. It is not known, however, if PRR changes its expression or is activated during normal or PE-complicated pregnancy on tissues intimately related to hypertension. So, the aim of this work was to describe PRR expression during normal and hypertensive pregnancy in rats. Methods: We used a subrenal aortic coarctation (SRAC) model in rats. Atria, septum and ventricular heart tissue, aorta and renal tissue samples were homogenized and immunoblotted using anti-PRR and anti-PLZF antibodies. We also measured gene expression by RT-PCR. Key findings: Hypertension and proteinuria were observed in SRAC-pregnant rats. In pregnant, non-SRAC rats, PRR showed a higher expression of both, gene and protein compared to non-pregnant rats in heart, aorta and kidney tissues. PE induces a very high expression of PRR in cardiac tissues and, on the contrary, decreases PRR expression in both, aorta and kidney. PLZF, a marker of PRR function, was augmented only in aorta and kidney in non-SRAC pregnant rats. In SRAC-pregnant rats, PLZF increment disappeared. Significance: These findings indicate that PRR expression changes differently during pregnancy and PE in tissues related to cardiovascular functions and suggest a probable participation of the receptor during normal and preeclamptic pregnancy in the rat.

AB - © 2018 Elsevier Inc. Aims: Pregnancy is a physiological stage with profound cardiovascular changes leading to hypotension. Preeclampsia (PE) reverts these normal changes inducing hypertension. Renin-angiotensin system (RAS) has been related in PE genesis. It has been reported a novel receptor in the system, the Prorenin/Renin receptor (PRR), with several roles in renal and cardiovascular illnesses. It is not known, however, if PRR changes its expression or is activated during normal or PE-complicated pregnancy on tissues intimately related to hypertension. So, the aim of this work was to describe PRR expression during normal and hypertensive pregnancy in rats. Methods: We used a subrenal aortic coarctation (SRAC) model in rats. Atria, septum and ventricular heart tissue, aorta and renal tissue samples were homogenized and immunoblotted using anti-PRR and anti-PLZF antibodies. We also measured gene expression by RT-PCR. Key findings: Hypertension and proteinuria were observed in SRAC-pregnant rats. In pregnant, non-SRAC rats, PRR showed a higher expression of both, gene and protein compared to non-pregnant rats in heart, aorta and kidney tissues. PE induces a very high expression of PRR in cardiac tissues and, on the contrary, decreases PRR expression in both, aorta and kidney. PLZF, a marker of PRR function, was augmented only in aorta and kidney in non-SRAC pregnant rats. In SRAC-pregnant rats, PLZF increment disappeared. Significance: These findings indicate that PRR expression changes differently during pregnancy and PE in tissues related to cardiovascular functions and suggest a probable participation of the receptor during normal and preeclamptic pregnancy in the rat.

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