Abstract
| Original language | American English |
|---|---|
| Pages (from-to) | 22-28 |
| Number of pages | 19 |
| Journal | Life Sciences |
| DOIs | |
| State | Published - 1 Jan 2019 |
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(Pro)renin/renin receptor expression during normal and preeclamptic pregnancy in rats. / Avila-Ramírez, M. A.; Esteban-Martínez, R. L.; López-Moctezuma, E.; Anguiano-Robledo, L.; Hernández-Campos, M. E.; López-Sánchez, P.
In: Life Sciences, 01.01.2019, p. 22-28.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - (Pro)renin/renin receptor expression during normal and preeclamptic pregnancy in rats
AU - Avila-Ramírez, M. A.
AU - Esteban-Martínez, R. L.
AU - López-Moctezuma, E.
AU - Anguiano-Robledo, L.
AU - Hernández-Campos, M. E.
AU - López-Sánchez, P.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - © 2018 Elsevier Inc. Aims: Pregnancy is a physiological stage with profound cardiovascular changes leading to hypotension. Preeclampsia (PE) reverts these normal changes inducing hypertension. Renin-angiotensin system (RAS) has been related in PE genesis. It has been reported a novel receptor in the system, the Prorenin/Renin receptor (PRR), with several roles in renal and cardiovascular illnesses. It is not known, however, if PRR changes its expression or is activated during normal or PE-complicated pregnancy on tissues intimately related to hypertension. So, the aim of this work was to describe PRR expression during normal and hypertensive pregnancy in rats. Methods: We used a subrenal aortic coarctation (SRAC) model in rats. Atria, septum and ventricular heart tissue, aorta and renal tissue samples were homogenized and immunoblotted using anti-PRR and anti-PLZF antibodies. We also measured gene expression by RT-PCR. Key findings: Hypertension and proteinuria were observed in SRAC-pregnant rats. In pregnant, non-SRAC rats, PRR showed a higher expression of both, gene and protein compared to non-pregnant rats in heart, aorta and kidney tissues. PE induces a very high expression of PRR in cardiac tissues and, on the contrary, decreases PRR expression in both, aorta and kidney. PLZF, a marker of PRR function, was augmented only in aorta and kidney in non-SRAC pregnant rats. In SRAC-pregnant rats, PLZF increment disappeared. Significance: These findings indicate that PRR expression changes differently during pregnancy and PE in tissues related to cardiovascular functions and suggest a probable participation of the receptor during normal and preeclamptic pregnancy in the rat.
AB - © 2018 Elsevier Inc. Aims: Pregnancy is a physiological stage with profound cardiovascular changes leading to hypotension. Preeclampsia (PE) reverts these normal changes inducing hypertension. Renin-angiotensin system (RAS) has been related in PE genesis. It has been reported a novel receptor in the system, the Prorenin/Renin receptor (PRR), with several roles in renal and cardiovascular illnesses. It is not known, however, if PRR changes its expression or is activated during normal or PE-complicated pregnancy on tissues intimately related to hypertension. So, the aim of this work was to describe PRR expression during normal and hypertensive pregnancy in rats. Methods: We used a subrenal aortic coarctation (SRAC) model in rats. Atria, septum and ventricular heart tissue, aorta and renal tissue samples were homogenized and immunoblotted using anti-PRR and anti-PLZF antibodies. We also measured gene expression by RT-PCR. Key findings: Hypertension and proteinuria were observed in SRAC-pregnant rats. In pregnant, non-SRAC rats, PRR showed a higher expression of both, gene and protein compared to non-pregnant rats in heart, aorta and kidney tissues. PE induces a very high expression of PRR in cardiac tissues and, on the contrary, decreases PRR expression in both, aorta and kidney. PLZF, a marker of PRR function, was augmented only in aorta and kidney in non-SRAC pregnant rats. In SRAC-pregnant rats, PLZF increment disappeared. Significance: These findings indicate that PRR expression changes differently during pregnancy and PE in tissues related to cardiovascular functions and suggest a probable participation of the receptor during normal and preeclamptic pregnancy in the rat.
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U2 - 10.1016/j.lfs.2018.11.017
DO - 10.1016/j.lfs.2018.11.017
M3 - Article
SP - 22
EP - 28
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
ER -