Promysalin, a salicylate-containing pseudomonas putida antibiotic, promotes surface colonization and selectively targets other pseudomonas

Wen Li, Paulina Estrada-De Los Santos, Sandra Matthijs, Guan Lin Xie, Roger Busson, Pierre Cornelis, Jef Rozenski, René De Mot

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Under control of the Gac regulatory system, Pseudomonas putida RW10S1 produces promysalin to promote its own swarming and biofilm formation, and to selectively inhibit many other pseudomonads, including the opportunistic pathogen Pseudomonas aeruginosa. This amphipathic antibiotic is composed of salicylic acid and 2,8-dihydroxymyristamide bridged by a unique 2-pyrroline-5-carboxyl moiety. In addition to enzymes for salicylic acid synthesis and activation, the biosynthetic gene cluster encodes divergent type II fatty acid biosynthesis components, unusual fatty acid-tailoring enzymes (two Rieske-type oxygenases and an amidotransferase), an enzyme resembling a proline-loading module of nonribosomal peptide synthetases, and the first prokaryotic member of the BAHD family of plant acyltransferases. Identification of biosynthetic intermediates enabled to propose a pathway for synthesis of this bacterial colonization factor. © 2011 Elsevier Ltd All rights reserved.
Original languageAmerican English
Pages (from-to)1320-1330
Number of pages11
JournalChemistry and Biology
DOIs
StatePublished - 28 Oct 2011
Externally publishedYes

Fingerprint

Pseudomonas putida
Salicylates
Pseudomonas
Salicylic Acid
Anti-Bacterial Agents
Enzymes
Fatty Acids
Peptide Synthases
Acyltransferases
Oxygenases
Biosynthesis
Biofilms
Pathogens
Multigene Family
Proline
Pseudomonas aeruginosa
Genes
Chemical activation
promysalin

Cite this

Li, Wen ; Estrada-De Los Santos, Paulina ; Matthijs, Sandra ; Xie, Guan Lin ; Busson, Roger ; Cornelis, Pierre ; Rozenski, Jef ; De Mot, René. / Promysalin, a salicylate-containing pseudomonas putida antibiotic, promotes surface colonization and selectively targets other pseudomonas. In: Chemistry and Biology. 2011 ; pp. 1320-1330.
@article{1e30bd4a58d0441d9578c7d2e3cf54ce,
title = "Promysalin, a salicylate-containing pseudomonas putida antibiotic, promotes surface colonization and selectively targets other pseudomonas",
abstract = "Under control of the Gac regulatory system, Pseudomonas putida RW10S1 produces promysalin to promote its own swarming and biofilm formation, and to selectively inhibit many other pseudomonads, including the opportunistic pathogen Pseudomonas aeruginosa. This amphipathic antibiotic is composed of salicylic acid and 2,8-dihydroxymyristamide bridged by a unique 2-pyrroline-5-carboxyl moiety. In addition to enzymes for salicylic acid synthesis and activation, the biosynthetic gene cluster encodes divergent type II fatty acid biosynthesis components, unusual fatty acid-tailoring enzymes (two Rieske-type oxygenases and an amidotransferase), an enzyme resembling a proline-loading module of nonribosomal peptide synthetases, and the first prokaryotic member of the BAHD family of plant acyltransferases. Identification of biosynthetic intermediates enabled to propose a pathway for synthesis of this bacterial colonization factor. {\circledC} 2011 Elsevier Ltd All rights reserved.",
author = "Wen Li and {Estrada-De Los Santos}, Paulina and Sandra Matthijs and Xie, {Guan Lin} and Roger Busson and Pierre Cornelis and Jef Rozenski and {De Mot}, Ren{\'e}",
year = "2011",
month = "10",
day = "28",
doi = "10.1016/j.chembiol.2011.08.006",
language = "American English",
pages = "1320--1330",
journal = "Chemistry and Biology",
issn = "1074-5521",
publisher = "Elsevier Inc.",

}

Promysalin, a salicylate-containing pseudomonas putida antibiotic, promotes surface colonization and selectively targets other pseudomonas. / Li, Wen; Estrada-De Los Santos, Paulina; Matthijs, Sandra; Xie, Guan Lin; Busson, Roger; Cornelis, Pierre; Rozenski, Jef; De Mot, René.

In: Chemistry and Biology, 28.10.2011, p. 1320-1330.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Promysalin, a salicylate-containing pseudomonas putida antibiotic, promotes surface colonization and selectively targets other pseudomonas

AU - Li, Wen

AU - Estrada-De Los Santos, Paulina

AU - Matthijs, Sandra

AU - Xie, Guan Lin

AU - Busson, Roger

AU - Cornelis, Pierre

AU - Rozenski, Jef

AU - De Mot, René

PY - 2011/10/28

Y1 - 2011/10/28

N2 - Under control of the Gac regulatory system, Pseudomonas putida RW10S1 produces promysalin to promote its own swarming and biofilm formation, and to selectively inhibit many other pseudomonads, including the opportunistic pathogen Pseudomonas aeruginosa. This amphipathic antibiotic is composed of salicylic acid and 2,8-dihydroxymyristamide bridged by a unique 2-pyrroline-5-carboxyl moiety. In addition to enzymes for salicylic acid synthesis and activation, the biosynthetic gene cluster encodes divergent type II fatty acid biosynthesis components, unusual fatty acid-tailoring enzymes (two Rieske-type oxygenases and an amidotransferase), an enzyme resembling a proline-loading module of nonribosomal peptide synthetases, and the first prokaryotic member of the BAHD family of plant acyltransferases. Identification of biosynthetic intermediates enabled to propose a pathway for synthesis of this bacterial colonization factor. © 2011 Elsevier Ltd All rights reserved.

AB - Under control of the Gac regulatory system, Pseudomonas putida RW10S1 produces promysalin to promote its own swarming and biofilm formation, and to selectively inhibit many other pseudomonads, including the opportunistic pathogen Pseudomonas aeruginosa. This amphipathic antibiotic is composed of salicylic acid and 2,8-dihydroxymyristamide bridged by a unique 2-pyrroline-5-carboxyl moiety. In addition to enzymes for salicylic acid synthesis and activation, the biosynthetic gene cluster encodes divergent type II fatty acid biosynthesis components, unusual fatty acid-tailoring enzymes (two Rieske-type oxygenases and an amidotransferase), an enzyme resembling a proline-loading module of nonribosomal peptide synthetases, and the first prokaryotic member of the BAHD family of plant acyltransferases. Identification of biosynthetic intermediates enabled to propose a pathway for synthesis of this bacterial colonization factor. © 2011 Elsevier Ltd All rights reserved.

U2 - 10.1016/j.chembiol.2011.08.006

DO - 10.1016/j.chembiol.2011.08.006

M3 - Article

SP - 1320

EP - 1330

JO - Chemistry and Biology

JF - Chemistry and Biology

SN - 1074-5521

ER -