TY - JOUR
T1 - Production, preparation and characterization of microalgae-based biopolymer as a potential bioactive film
AU - Morales-Jiménez, Mónica
AU - Gouveia, Luisa
AU - Yáñez-Fernández, Jorge
AU - Castro-Muñoz, Roberto
AU - Barragán-Huerta, Blanca Estela
N1 - Publisher Copyright:
© 2020 by the authors.
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Six microalgae strains were screened according to their biomass productivity and polymer synthesis, showing biomass productivity between 0.14 and 0.68 g/(Ld) for a 21-day growth period. Extracellular biopolymers from the spent culture media of Nostoc sp. (No), Synechocystis sp. (Sy), and Porphyridium purpureum (Pp) was obtained, and the yields of the clean biopolymer were 323, 204, and 83 mg/L, respectively. The crude biopolymer was cleaned up using a solid-phase extraction technique. The emulsification index E24 values for the clean biopolymer were 77.5%, 68.8%, and 73.3% at 0.323, 0.083, and 0.204 mg/mL, respectively. The clean biopolymer of the No strain showed the highest fungal growth inhibition against Fusarium verticillioides (70.2%) and Fusarium sp. (61.4%) at 2.24 mg/mL. In general, transparent and flexible biofilms were prepared using biopolymers of No and Pp. The microstructural analysis revealed the presence of pores and cracks in the biofilms, and the average roughness Ra values are 68.6 and 86.4 nm for No and Pp, respectively, and the root mean square roughness Rq values are 86.2 and 107.2 nm for No and Pp, respectively.
AB - Six microalgae strains were screened according to their biomass productivity and polymer synthesis, showing biomass productivity between 0.14 and 0.68 g/(Ld) for a 21-day growth period. Extracellular biopolymers from the spent culture media of Nostoc sp. (No), Synechocystis sp. (Sy), and Porphyridium purpureum (Pp) was obtained, and the yields of the clean biopolymer were 323, 204, and 83 mg/L, respectively. The crude biopolymer was cleaned up using a solid-phase extraction technique. The emulsification index E24 values for the clean biopolymer were 77.5%, 68.8%, and 73.3% at 0.323, 0.083, and 0.204 mg/mL, respectively. The clean biopolymer of the No strain showed the highest fungal growth inhibition against Fusarium verticillioides (70.2%) and Fusarium sp. (61.4%) at 2.24 mg/mL. In general, transparent and flexible biofilms were prepared using biopolymers of No and Pp. The microstructural analysis revealed the presence of pores and cracks in the biofilms, and the average roughness Ra values are 68.6 and 86.4 nm for No and Pp, respectively, and the root mean square roughness Rq values are 86.2 and 107.2 nm for No and Pp, respectively.
KW - Antifungal
KW - Biofilms
KW - Biopolymer
KW - Emulsification
KW - Microalgae
UR - http://www.scopus.com/inward/record.url?scp=85080922516&partnerID=8YFLogxK
U2 - 10.3390/coatings10020120
DO - 10.3390/coatings10020120
M3 - Artículo
AN - SCOPUS:85080922516
SN - 2079-6412
VL - 10
JO - Coatings
JF - Coatings
IS - 2
M1 - 120
ER -