TY - JOUR
T1 - Production of polyhydroxybutyrate (PHB) by a novel Klebsiella pneumoniae strain using low-cost media from fruit peel residues
AU - Valdez-Calderón, A.
AU - Barraza-Salas, M.
AU - Quezada-Cruz, M.
AU - Islas-Ponce, M. A.
AU - Angeles-Padilla, A. F.
AU - Carrillo-Ibarra, S.
AU - Rodríguez, M.
AU - Rojas-Avelizapa, N. G.
AU - Garrido-Hernández, A.
AU - Rivas-Castillo, A. M.
N1 - Publisher Copyright:
© 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2022/11
Y1 - 2022/11
N2 - Plastics are widely used for various applications. Once discarded, it is commonly known that they represent a high environmental threat due to their slow degradation; for this reason, there is an imminent need to replace these products with eco-friendlier ones. In the present work, four bacterial polyhydroxybutyrate (PHB) producers, two consortia, and two isolated strains were successfully recovered from the facilities of a paper-manufacturing industry. Spectroscopic studies of the biopolymers obtained from these bacteria corroborated their PHB production capabilities, ranging from 4.04 ± 0.16 to 23.82 ± 3.39 g/L. The characterization of the isolate that presented the highest production yield initially coded as E22 led to the identification of a Klebsiella pneumoniae strain, which, compared with other PHA bacterial producers reported to date, could be considered with high production potential. The strain E22 was grown in 5 different media prepared from fruit peel residues of banana, orange, papaya, watermelon, and melon, to determine its growth and PHA production capabilities in these low-cost media. The results obtained show different bacterial growth yields among the media tested, although PHB production yields and productivities were similar in all these low-cost media. Cellular accumulation of the biopolymer was higher in watermelon peel medium (8.4 × 10−10 g/CFU). These results reveal the potential of K. pneumoniae E22 for PHB production applications and establish encouraging alternatives to be broader explored regarding low-cost media that could enhance the scale-up of bacterial PHA production processes. [Figure not available: see fulltext.].
AB - Plastics are widely used for various applications. Once discarded, it is commonly known that they represent a high environmental threat due to their slow degradation; for this reason, there is an imminent need to replace these products with eco-friendlier ones. In the present work, four bacterial polyhydroxybutyrate (PHB) producers, two consortia, and two isolated strains were successfully recovered from the facilities of a paper-manufacturing industry. Spectroscopic studies of the biopolymers obtained from these bacteria corroborated their PHB production capabilities, ranging from 4.04 ± 0.16 to 23.82 ± 3.39 g/L. The characterization of the isolate that presented the highest production yield initially coded as E22 led to the identification of a Klebsiella pneumoniae strain, which, compared with other PHA bacterial producers reported to date, could be considered with high production potential. The strain E22 was grown in 5 different media prepared from fruit peel residues of banana, orange, papaya, watermelon, and melon, to determine its growth and PHA production capabilities in these low-cost media. The results obtained show different bacterial growth yields among the media tested, although PHB production yields and productivities were similar in all these low-cost media. Cellular accumulation of the biopolymer was higher in watermelon peel medium (8.4 × 10−10 g/CFU). These results reveal the potential of K. pneumoniae E22 for PHB production applications and establish encouraging alternatives to be broader explored regarding low-cost media that could enhance the scale-up of bacterial PHA production processes. [Figure not available: see fulltext.].
KW - Biopolymers
KW - Fruit residues
KW - Klebsiella pneumoniae
KW - PHB
KW - Polyhydroxyalkanoates
UR - http://www.scopus.com/inward/record.url?scp=85096204213&partnerID=8YFLogxK
U2 - 10.1007/s13399-020-01147-5
DO - 10.1007/s13399-020-01147-5
M3 - Artículo
AN - SCOPUS:85096204213
SN - 2190-6815
VL - 12
SP - 4925
EP - 4938
JO - Biomass Conversion and Biorefinery
JF - Biomass Conversion and Biorefinery
IS - 11
ER -