Production of hybrid strains among Pleutorus and Lentinula and evaluation of their mycelial growth kinetics on malt extract agar and wheat grain using the Gompertz and Hill models

Juan Diego Valenzuela Cobos, Enrique Durán Páramo, Ramón Villanueva Arce, María Eugenia Garín Aguilar, Abraham Sánchez Hernández, Hermilo Leal Lara, Gustavo Valencia Del Toro

Research output: Contribution to journalArticle

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Abstract

Three inter-generic hybrid strains were obtained by pairing compatible neohaplonts recovered by dedikaryotization of four parental strains, i.e. Pleurotus ostreatus (CC060), Pleurotus djamor (CC051) and Lentinula edodes (CC003 and CC004). Fifteen neohaplonts were recovered employing homogenization time periods for Pleurotus spp. since 60 to 90 s, and for Lentinula edodes strains time periods ranged from 5 to 20 s, and incubation at 28 °C in a peptone-glucose solution (PGS). The mycelial growth of the parental and hybrid strains was determined on malt extract agar (MEA) and wheat grain by calculating the parameters A, B and C of nonlinear regression models. For the parameter A, the mycelial growth on MEA showed values ranged from 1.08 to 1.28, and for the parameter B since 5.65 to 20.85. On the other hand, the A values by mycelium growth on wheat grain ranged from 1.57 to 16.13, for B since 28.36 to 86.53 and for C were in an interval from 11.89 to 44.24. Parental and hybrid strains presented instantaneous velocity values on MEA since 2.88 to 7.26 cm2•day-1, whereas on wheat grain were in an interval from 5.45 to 10.05 cm3•day-1. Furthemore, the μmaxvalues on MEA and wheat grain were calculated by using the Gompertz model and the λ values on both medium were estimated by using the Hill model, the μmaxvalues on MEA ranged from 0.26 to 1.69 day-1and λ values in an interval from 0.41 to 2.74, whereas the μmaxvalues on wheat grain ranged from 0.05 to 0.43 day-1and λ since 1.82 to 28 h. Moreover, the estimated equations based on nonlinear models were used to calculate the μmaxvalues on MEA and wheat grain of the strains and the λ values were obtained by using the μmaxvalues of the proposed equations on the Hill model, the μmaxvalues on MEA ranged from 0.44 to 1.27 day-1andthe λ values between 0.57 to 1.72 h, while the μmaxvalues on wheat grain ranged from 0.04 to 0.64 day-1and λ values in an interval from 1.26 to 20.11 h. The results evidenced that the hybrid strains of PleurotusxLentinula presented highest rate of growth in comparison with the parental, encouraging the production of hybrid strain and their use in the industrial field.
Original languageAmerican English
Pages (from-to)927-935
Number of pages9
JournalEmirates Journal of Food and Agriculture
DOIs
StatePublished - 1 Dec 2017

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Lentinula
malt extract
Triticum
Agar
agar
kinetics
wheat
Pleurotus
Growth
Shiitake Mushrooms
Nonlinear Dynamics
Lentinula edodes
Pleurotus djamor
Peptones
Mycelium
Pleurotus ostreatus
peptones
nonlinear models
homogenization
mycelium

Cite this

Cobos, Juan Diego Valenzuela ; Páramo, Enrique Durán ; Arce, Ramón Villanueva ; Aguilar, María Eugenia Garín ; Hernández, Abraham Sánchez ; Lara, Hermilo Leal ; Del Toro, Gustavo Valencia. / Production of hybrid strains among Pleutorus and Lentinula and evaluation of their mycelial growth kinetics on malt extract agar and wheat grain using the Gompertz and Hill models. In: Emirates Journal of Food and Agriculture. 2017 ; pp. 927-935.
@article{1239230fd4a84391863a0c8e0aaffb50,
title = "Production of hybrid strains among Pleutorus and Lentinula and evaluation of their mycelial growth kinetics on malt extract agar and wheat grain using the Gompertz and Hill models",
abstract = "Three inter-generic hybrid strains were obtained by pairing compatible neohaplonts recovered by dedikaryotization of four parental strains, i.e. Pleurotus ostreatus (CC060), Pleurotus djamor (CC051) and Lentinula edodes (CC003 and CC004). Fifteen neohaplonts were recovered employing homogenization time periods for Pleurotus spp. since 60 to 90 s, and for Lentinula edodes strains time periods ranged from 5 to 20 s, and incubation at 28 °C in a peptone-glucose solution (PGS). The mycelial growth of the parental and hybrid strains was determined on malt extract agar (MEA) and wheat grain by calculating the parameters A, B and C of nonlinear regression models. For the parameter A, the mycelial growth on MEA showed values ranged from 1.08 to 1.28, and for the parameter B since 5.65 to 20.85. On the other hand, the A values by mycelium growth on wheat grain ranged from 1.57 to 16.13, for B since 28.36 to 86.53 and for C were in an interval from 11.89 to 44.24. Parental and hybrid strains presented instantaneous velocity values on MEA since 2.88 to 7.26 cm2•day-1, whereas on wheat grain were in an interval from 5.45 to 10.05 cm3•day-1. Furthemore, the μmaxvalues on MEA and wheat grain were calculated by using the Gompertz model and the λ values on both medium were estimated by using the Hill model, the μmaxvalues on MEA ranged from 0.26 to 1.69 day-1and λ values in an interval from 0.41 to 2.74, whereas the μmaxvalues on wheat grain ranged from 0.05 to 0.43 day-1and λ since 1.82 to 28 h. Moreover, the estimated equations based on nonlinear models were used to calculate the μmaxvalues on MEA and wheat grain of the strains and the λ values were obtained by using the μmaxvalues of the proposed equations on the Hill model, the μmaxvalues on MEA ranged from 0.44 to 1.27 day-1andthe λ values between 0.57 to 1.72 h, while the μmaxvalues on wheat grain ranged from 0.04 to 0.64 day-1and λ values in an interval from 1.26 to 20.11 h. The results evidenced that the hybrid strains of PleurotusxLentinula presented highest rate of growth in comparison with the parental, encouraging the production of hybrid strain and their use in the industrial field.",
author = "Cobos, {Juan Diego Valenzuela} and P{\'a}ramo, {Enrique Dur{\'a}n} and Arce, {Ram{\'o}n Villanueva} and Aguilar, {Mar{\'i}a Eugenia Gar{\'i}n} and Hern{\'a}ndez, {Abraham S{\'a}nchez} and Lara, {Hermilo Leal} and {Del Toro}, {Gustavo Valencia}",
year = "2017",
month = "12",
day = "1",
doi = "10.9755/ejfa.2017.v29.i12.1563",
language = "American English",
pages = "927--935",
journal = "Emirates Journal of Food and Agriculture",
issn = "2079-052X",
publisher = "United Arab Emirates University",

}

Production of hybrid strains among Pleutorus and Lentinula and evaluation of their mycelial growth kinetics on malt extract agar and wheat grain using the Gompertz and Hill models. / Cobos, Juan Diego Valenzuela; Páramo, Enrique Durán; Arce, Ramón Villanueva; Aguilar, María Eugenia Garín; Hernández, Abraham Sánchez; Lara, Hermilo Leal; Del Toro, Gustavo Valencia.

In: Emirates Journal of Food and Agriculture, 01.12.2017, p. 927-935.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Production of hybrid strains among Pleutorus and Lentinula and evaluation of their mycelial growth kinetics on malt extract agar and wheat grain using the Gompertz and Hill models

AU - Cobos, Juan Diego Valenzuela

AU - Páramo, Enrique Durán

AU - Arce, Ramón Villanueva

AU - Aguilar, María Eugenia Garín

AU - Hernández, Abraham Sánchez

AU - Lara, Hermilo Leal

AU - Del Toro, Gustavo Valencia

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Three inter-generic hybrid strains were obtained by pairing compatible neohaplonts recovered by dedikaryotization of four parental strains, i.e. Pleurotus ostreatus (CC060), Pleurotus djamor (CC051) and Lentinula edodes (CC003 and CC004). Fifteen neohaplonts were recovered employing homogenization time periods for Pleurotus spp. since 60 to 90 s, and for Lentinula edodes strains time periods ranged from 5 to 20 s, and incubation at 28 °C in a peptone-glucose solution (PGS). The mycelial growth of the parental and hybrid strains was determined on malt extract agar (MEA) and wheat grain by calculating the parameters A, B and C of nonlinear regression models. For the parameter A, the mycelial growth on MEA showed values ranged from 1.08 to 1.28, and for the parameter B since 5.65 to 20.85. On the other hand, the A values by mycelium growth on wheat grain ranged from 1.57 to 16.13, for B since 28.36 to 86.53 and for C were in an interval from 11.89 to 44.24. Parental and hybrid strains presented instantaneous velocity values on MEA since 2.88 to 7.26 cm2•day-1, whereas on wheat grain were in an interval from 5.45 to 10.05 cm3•day-1. Furthemore, the μmaxvalues on MEA and wheat grain were calculated by using the Gompertz model and the λ values on both medium were estimated by using the Hill model, the μmaxvalues on MEA ranged from 0.26 to 1.69 day-1and λ values in an interval from 0.41 to 2.74, whereas the μmaxvalues on wheat grain ranged from 0.05 to 0.43 day-1and λ since 1.82 to 28 h. Moreover, the estimated equations based on nonlinear models were used to calculate the μmaxvalues on MEA and wheat grain of the strains and the λ values were obtained by using the μmaxvalues of the proposed equations on the Hill model, the μmaxvalues on MEA ranged from 0.44 to 1.27 day-1andthe λ values between 0.57 to 1.72 h, while the μmaxvalues on wheat grain ranged from 0.04 to 0.64 day-1and λ values in an interval from 1.26 to 20.11 h. The results evidenced that the hybrid strains of PleurotusxLentinula presented highest rate of growth in comparison with the parental, encouraging the production of hybrid strain and their use in the industrial field.

AB - Three inter-generic hybrid strains were obtained by pairing compatible neohaplonts recovered by dedikaryotization of four parental strains, i.e. Pleurotus ostreatus (CC060), Pleurotus djamor (CC051) and Lentinula edodes (CC003 and CC004). Fifteen neohaplonts were recovered employing homogenization time periods for Pleurotus spp. since 60 to 90 s, and for Lentinula edodes strains time periods ranged from 5 to 20 s, and incubation at 28 °C in a peptone-glucose solution (PGS). The mycelial growth of the parental and hybrid strains was determined on malt extract agar (MEA) and wheat grain by calculating the parameters A, B and C of nonlinear regression models. For the parameter A, the mycelial growth on MEA showed values ranged from 1.08 to 1.28, and for the parameter B since 5.65 to 20.85. On the other hand, the A values by mycelium growth on wheat grain ranged from 1.57 to 16.13, for B since 28.36 to 86.53 and for C were in an interval from 11.89 to 44.24. Parental and hybrid strains presented instantaneous velocity values on MEA since 2.88 to 7.26 cm2•day-1, whereas on wheat grain were in an interval from 5.45 to 10.05 cm3•day-1. Furthemore, the μmaxvalues on MEA and wheat grain were calculated by using the Gompertz model and the λ values on both medium were estimated by using the Hill model, the μmaxvalues on MEA ranged from 0.26 to 1.69 day-1and λ values in an interval from 0.41 to 2.74, whereas the μmaxvalues on wheat grain ranged from 0.05 to 0.43 day-1and λ since 1.82 to 28 h. Moreover, the estimated equations based on nonlinear models were used to calculate the μmaxvalues on MEA and wheat grain of the strains and the λ values were obtained by using the μmaxvalues of the proposed equations on the Hill model, the μmaxvalues on MEA ranged from 0.44 to 1.27 day-1andthe λ values between 0.57 to 1.72 h, while the μmaxvalues on wheat grain ranged from 0.04 to 0.64 day-1and λ values in an interval from 1.26 to 20.11 h. The results evidenced that the hybrid strains of PleurotusxLentinula presented highest rate of growth in comparison with the parental, encouraging the production of hybrid strain and their use in the industrial field.

U2 - 10.9755/ejfa.2017.v29.i12.1563

DO - 10.9755/ejfa.2017.v29.i12.1563

M3 - Article

SP - 927

EP - 935

JO - Emirates Journal of Food and Agriculture

JF - Emirates Journal of Food and Agriculture

SN - 2079-052X

ER -