Production of cellulases and xylanases under catabolic repression conditions from mutant PR-22 of Cellulomonas flavigena

Oscar A. Rojas-Rejón, Héctor M. Poggi-Varaldo, Ana C. Ramos-Valdivia, Alfredo Martínez-Jiménez, Eliseo Cristiani-Urbina, Mayra De La Torre Martínez, Teresa Ponce-Noyola

Research output: Contribution to conferencePaper

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Abstract

Derepressed mutant PR-22 was obtained by N-methyl-N′-nitro-N- nitrosoguanidine (MNNG) mutagenic treatment of Cellulomonas flavigena PN-120. This mutant improved its xylanolytic activity from 26.9 to 40 U mg -1 and cellulolytic activity from 1.9 to 4 U mg -1 ; this represented rates almost 2 and 1.5 times higher, respectively, compared to its parent strain growing in sugarcane bagasse. Either glucose or cellobiose was added to cultures of C. flavigena PN-120 and mutant PR-22 induced with sugarcane bagasse in batch culture. The inhibitory effect of glucose on xylanase activity was more noticeable for parent strain PN-120 than for mutant PR-22. When 20 mM glucose was added, the xylanolytic activity decreased 41% compared to the culture grown without glucose in mutant PR-22, whereas in the PN-120 strain the xylanolytic activity decreased by 49% at the same conditions compared to its own control. Addition of 10 and 15 mM of glucose did not adversely affect CMCase activity in PR-22, but glucose at 20 mM inhibited the enzymatic activity by 28%. The CMCase activity of the PN-120 strain was more sensitive to glucose than PR-22, with a reduction of CMCase activity in the range of 20-32%. Cellobiose had a more significant effect on xylanase and CMCase activities than glucose did in the mutant PR-22 and parent strain. Nevertheless, the activities under both conditions were always higher in the mutant PR-22 than in the PN-120 strain. Enzymatic saccharification experiments showed that it is possible to accumulate up to 10 g l -1 of total soluble sugars from pretreated sugarcane bagasse with the concentrated enzymatic crude extract from mutant PR-22. © 2010 Society for Industrial Microbiology.
Original languageAmerican English
Pages257-264
Number of pages230
DOIs
StatePublished - 1 Jan 2011
EventJournal of Industrial Microbiology and Biotechnology -
Duration: 1 Jan 2011 → …

Conference

ConferenceJournal of Industrial Microbiology and Biotechnology
Period1/01/11 → …

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Cellulomonas
Cellulases
Glucose
Saccharum
Cellobiose
Industrial Microbiology
Methylnitronitrosoguanidine
Batch Cell Culture Techniques
Complex Mixtures

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Rojas-Rejón, O. A., Poggi-Varaldo, H. M., Ramos-Valdivia, A. C., Martínez-Jiménez, A., Cristiani-Urbina, E., De La Torre Martínez, M., & Ponce-Noyola, T. (2011). Production of cellulases and xylanases under catabolic repression conditions from mutant PR-22 of Cellulomonas flavigena. 257-264. Paper presented at Journal of Industrial Microbiology and Biotechnology, . https://doi.org/10.1007/s10295-010-0821-7
Rojas-Rejón, Oscar A. ; Poggi-Varaldo, Héctor M. ; Ramos-Valdivia, Ana C. ; Martínez-Jiménez, Alfredo ; Cristiani-Urbina, Eliseo ; De La Torre Martínez, Mayra ; Ponce-Noyola, Teresa. / Production of cellulases and xylanases under catabolic repression conditions from mutant PR-22 of Cellulomonas flavigena. Paper presented at Journal of Industrial Microbiology and Biotechnology, .230 p.
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title = "Production of cellulases and xylanases under catabolic repression conditions from mutant PR-22 of Cellulomonas flavigena",
abstract = "Derepressed mutant PR-22 was obtained by N-methyl-N′-nitro-N- nitrosoguanidine (MNNG) mutagenic treatment of Cellulomonas flavigena PN-120. This mutant improved its xylanolytic activity from 26.9 to 40 U mg -1 and cellulolytic activity from 1.9 to 4 U mg -1 ; this represented rates almost 2 and 1.5 times higher, respectively, compared to its parent strain growing in sugarcane bagasse. Either glucose or cellobiose was added to cultures of C. flavigena PN-120 and mutant PR-22 induced with sugarcane bagasse in batch culture. The inhibitory effect of glucose on xylanase activity was more noticeable for parent strain PN-120 than for mutant PR-22. When 20 mM glucose was added, the xylanolytic activity decreased 41{\%} compared to the culture grown without glucose in mutant PR-22, whereas in the PN-120 strain the xylanolytic activity decreased by 49{\%} at the same conditions compared to its own control. Addition of 10 and 15 mM of glucose did not adversely affect CMCase activity in PR-22, but glucose at 20 mM inhibited the enzymatic activity by 28{\%}. The CMCase activity of the PN-120 strain was more sensitive to glucose than PR-22, with a reduction of CMCase activity in the range of 20-32{\%}. Cellobiose had a more significant effect on xylanase and CMCase activities than glucose did in the mutant PR-22 and parent strain. Nevertheless, the activities under both conditions were always higher in the mutant PR-22 than in the PN-120 strain. Enzymatic saccharification experiments showed that it is possible to accumulate up to 10 g l -1 of total soluble sugars from pretreated sugarcane bagasse with the concentrated enzymatic crude extract from mutant PR-22. {\circledC} 2010 Society for Industrial Microbiology.",
author = "Rojas-Rej{\'o}n, {Oscar A.} and Poggi-Varaldo, {H{\'e}ctor M.} and Ramos-Valdivia, {Ana C.} and Alfredo Mart{\'i}nez-Jim{\'e}nez and Eliseo Cristiani-Urbina and {De La Torre Mart{\'i}nez}, Mayra and Teresa Ponce-Noyola",
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Rojas-Rejón, OA, Poggi-Varaldo, HM, Ramos-Valdivia, AC, Martínez-Jiménez, A, Cristiani-Urbina, E, De La Torre Martínez, M & Ponce-Noyola, T 2011, 'Production of cellulases and xylanases under catabolic repression conditions from mutant PR-22 of Cellulomonas flavigena', Paper presented at Journal of Industrial Microbiology and Biotechnology, 1/01/11 pp. 257-264. https://doi.org/10.1007/s10295-010-0821-7

Production of cellulases and xylanases under catabolic repression conditions from mutant PR-22 of Cellulomonas flavigena. / Rojas-Rejón, Oscar A.; Poggi-Varaldo, Héctor M.; Ramos-Valdivia, Ana C.; Martínez-Jiménez, Alfredo; Cristiani-Urbina, Eliseo; De La Torre Martínez, Mayra; Ponce-Noyola, Teresa.

2011. 257-264 Paper presented at Journal of Industrial Microbiology and Biotechnology, .

Research output: Contribution to conferencePaper

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T1 - Production of cellulases and xylanases under catabolic repression conditions from mutant PR-22 of Cellulomonas flavigena

AU - Rojas-Rejón, Oscar A.

AU - Poggi-Varaldo, Héctor M.

AU - Ramos-Valdivia, Ana C.

AU - Martínez-Jiménez, Alfredo

AU - Cristiani-Urbina, Eliseo

AU - De La Torre Martínez, Mayra

AU - Ponce-Noyola, Teresa

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Derepressed mutant PR-22 was obtained by N-methyl-N′-nitro-N- nitrosoguanidine (MNNG) mutagenic treatment of Cellulomonas flavigena PN-120. This mutant improved its xylanolytic activity from 26.9 to 40 U mg -1 and cellulolytic activity from 1.9 to 4 U mg -1 ; this represented rates almost 2 and 1.5 times higher, respectively, compared to its parent strain growing in sugarcane bagasse. Either glucose or cellobiose was added to cultures of C. flavigena PN-120 and mutant PR-22 induced with sugarcane bagasse in batch culture. The inhibitory effect of glucose on xylanase activity was more noticeable for parent strain PN-120 than for mutant PR-22. When 20 mM glucose was added, the xylanolytic activity decreased 41% compared to the culture grown without glucose in mutant PR-22, whereas in the PN-120 strain the xylanolytic activity decreased by 49% at the same conditions compared to its own control. Addition of 10 and 15 mM of glucose did not adversely affect CMCase activity in PR-22, but glucose at 20 mM inhibited the enzymatic activity by 28%. The CMCase activity of the PN-120 strain was more sensitive to glucose than PR-22, with a reduction of CMCase activity in the range of 20-32%. Cellobiose had a more significant effect on xylanase and CMCase activities than glucose did in the mutant PR-22 and parent strain. Nevertheless, the activities under both conditions were always higher in the mutant PR-22 than in the PN-120 strain. Enzymatic saccharification experiments showed that it is possible to accumulate up to 10 g l -1 of total soluble sugars from pretreated sugarcane bagasse with the concentrated enzymatic crude extract from mutant PR-22. © 2010 Society for Industrial Microbiology.

AB - Derepressed mutant PR-22 was obtained by N-methyl-N′-nitro-N- nitrosoguanidine (MNNG) mutagenic treatment of Cellulomonas flavigena PN-120. This mutant improved its xylanolytic activity from 26.9 to 40 U mg -1 and cellulolytic activity from 1.9 to 4 U mg -1 ; this represented rates almost 2 and 1.5 times higher, respectively, compared to its parent strain growing in sugarcane bagasse. Either glucose or cellobiose was added to cultures of C. flavigena PN-120 and mutant PR-22 induced with sugarcane bagasse in batch culture. The inhibitory effect of glucose on xylanase activity was more noticeable for parent strain PN-120 than for mutant PR-22. When 20 mM glucose was added, the xylanolytic activity decreased 41% compared to the culture grown without glucose in mutant PR-22, whereas in the PN-120 strain the xylanolytic activity decreased by 49% at the same conditions compared to its own control. Addition of 10 and 15 mM of glucose did not adversely affect CMCase activity in PR-22, but glucose at 20 mM inhibited the enzymatic activity by 28%. The CMCase activity of the PN-120 strain was more sensitive to glucose than PR-22, with a reduction of CMCase activity in the range of 20-32%. Cellobiose had a more significant effect on xylanase and CMCase activities than glucose did in the mutant PR-22 and parent strain. Nevertheless, the activities under both conditions were always higher in the mutant PR-22 than in the PN-120 strain. Enzymatic saccharification experiments showed that it is possible to accumulate up to 10 g l -1 of total soluble sugars from pretreated sugarcane bagasse with the concentrated enzymatic crude extract from mutant PR-22. © 2010 Society for Industrial Microbiology.

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Rojas-Rejón OA, Poggi-Varaldo HM, Ramos-Valdivia AC, Martínez-Jiménez A, Cristiani-Urbina E, De La Torre Martínez M et al. Production of cellulases and xylanases under catabolic repression conditions from mutant PR-22 of Cellulomonas flavigena. 2011. Paper presented at Journal of Industrial Microbiology and Biotechnology, . https://doi.org/10.1007/s10295-010-0821-7