Abstract
The production of biomass and ligninolytic enzymes by Pleurotus ostreatus was analysed in synthetic medium with yeast extract and different glucose concentrations (0.5 - 20 g/l), at different pH (3.5-6.5) and incubation temperatures (23-32°C). The best culture condition were: initial glucose concentration of 5 g/l, initial pH between 5.5-6.5 and incubation temperature between 26-29°C. The saturation constant for glucose (Ks) was 1.75 g/l. The biomass concentration reached 8.6 g/l with a glucose addition of 20.0 g/l to the culture medium. The control of pH allowed an increment of 0.5 g/l of biomass concentration. The bioreactor produced pellets with a homogeneuous distribution of diameter size of 3.4 ± 0.2 mm. Approximately, 307 U/l of laccase and 0.41 U/l of manganese peroxidase were obtained in extracellular liquid medium and 0.015 U/g of laccase and 0.809 U/g of manganese peroxidase were obtained in solid substrate. Lignin peroxidase activity was not detected at any condition.
Translated title of the contribution | Production of biomass and ligninolytic enzymes by Pleurotus ostreatus in submerged culture |
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Original language | Spanish |
Pages (from-to) | 302-306 |
Number of pages | 5 |
Journal | Revista Iberoamericana de Micologia |
Volume | 15 |
Issue number | 4 |
State | Published - 1998 |
Externally published | Yes |