TY - JOUR
T1 - Preventive effect of tert-butylhydroquinone on cisplatin-induced nephrotoxicity in rats
AU - Pérez-Rojas, Jazmin M.
AU - Guerrero-Beltrán, Carlos Enrique
AU - Cruz, Cristino
AU - Sánchez-González, Dolores J.
AU - Martínez-Martínez, Claudia M.
AU - Pedraza-Chaverri, José
N1 - Funding Information:
The authors thank the financial support of CONACYT 129838 and DGAPA 201910. Jazmin M. Pérez-Rojas received a postdoctoral fellowship from UNAM through DGAPA.
PY - 2011/10
Y1 - 2011/10
N2 - Cis-diamminedichloroplatinum II (CDDP)-induced nephrotoxicity is associated with the overproduction of reactive oxygen species. tert-Butylhydroquinone (tBHQ) is a compound widely used as food antioxidant. The purpose of this study was to investigate the ability of tBHQ to prevent the nephrotoxic effect of CDDP in rats as well as the mechanisms involved. Thirty-six Wistar rats divided in the following groups were used: control, tBHQ (12.5. mg/kg), CDDP (7.5. mg/kg) and tBHQ. +. CDDP. Twenty-four h urine was collected at the beginning and at the end of the experiment and the rats were sacrificed 72. h after CDDP-administration. Histological studies were performed and markers of renal function and oxidative/nitrosative stress were measured. In addition, the activity of the following antioxidant enzymes was measured: glutathione peroxidase (GPx), superoxide dismutase (SOD), glutathione reductase (GR) and glutathione-S-transferase (GST). CDDP-induced renal dysfunction, structural damage and oxidative/nitrosative were prevented by tBHQ. In addition, tBHQ completely prevented the CDDP-induced fall in GPx and GST activities. In conclusion, the present study indicates that the antioxidant activity of tBHQ is associated with its nephroprotective effect against CDDP-induced acute kidney injury in rats.
AB - Cis-diamminedichloroplatinum II (CDDP)-induced nephrotoxicity is associated with the overproduction of reactive oxygen species. tert-Butylhydroquinone (tBHQ) is a compound widely used as food antioxidant. The purpose of this study was to investigate the ability of tBHQ to prevent the nephrotoxic effect of CDDP in rats as well as the mechanisms involved. Thirty-six Wistar rats divided in the following groups were used: control, tBHQ (12.5. mg/kg), CDDP (7.5. mg/kg) and tBHQ. +. CDDP. Twenty-four h urine was collected at the beginning and at the end of the experiment and the rats were sacrificed 72. h after CDDP-administration. Histological studies were performed and markers of renal function and oxidative/nitrosative stress were measured. In addition, the activity of the following antioxidant enzymes was measured: glutathione peroxidase (GPx), superoxide dismutase (SOD), glutathione reductase (GR) and glutathione-S-transferase (GST). CDDP-induced renal dysfunction, structural damage and oxidative/nitrosative were prevented by tBHQ. In addition, tBHQ completely prevented the CDDP-induced fall in GPx and GST activities. In conclusion, the present study indicates that the antioxidant activity of tBHQ is associated with its nephroprotective effect against CDDP-induced acute kidney injury in rats.
KW - Cisplatin
KW - Kidney damage
KW - Oxidative stress
KW - Tert-Butylhydroquinone
UR - http://www.scopus.com/inward/record.url?scp=80051905926&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2011.07.008
DO - 10.1016/j.fct.2011.07.008
M3 - Artículo
C2 - 21802473
SN - 0278-6915
VL - 49
SP - 2631
EP - 2637
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
IS - 10
ER -