TY - JOUR
T1 - Prevention of aflatoxin B1-induced dna breaks by β-D-glucan
AU - Madrigal-Bujaidar, Eduardo
AU - Morales-González, José Antonio
AU - Sánchez-Gutiérrez, Manuel
AU - Izquierdo-Vega, Jeannett A.
AU - Reyes-Arellano, Alicia
AU - Álvarez-González, Isela
AU - Pérez-Pasten, Ricardo
AU - Madrigal-Santillán, Eduardo
N1 - Publisher Copyright:
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
PY - 2015/6/11
Y1 - 2015/6/11
N2 - Aflatoxins are a group of naturally-occurring carcinogens that are known to contaminate different human and animal foodstuffs. Aflatoxin B1 (AFB1) is the most genotoxic hepatocarcinogenic compound of all of the aflatoxins. In this report, we explore the capacity of β-D-glucan (Glu) to reduce the DNA damage induced by AFB1 in mouse hepatocytes. For this purpose, we applied the comet assay to groups of animals that were first administered Glu in three doses (100, 400 and 700 mg/kg bw, respectively) and, 20 min later, 1.0 mg/kg of AFB1. Liver cells were obtained at 4, 10 and 16 h after the chemical administration and examined. The results showed no protection of the damage induced byAFB1 with the low dose of the polysaccharide, but they did reveal antigenotoxic activity exerted by the two high doses. In addition, we induced a co-crystallization between both compounds, determined their fusion points and analyzed the molecules by UV spectroscopy. The data suggested the formation of a supramolecular complex between AFB1 and β-D-glucan.
AB - Aflatoxins are a group of naturally-occurring carcinogens that are known to contaminate different human and animal foodstuffs. Aflatoxin B1 (AFB1) is the most genotoxic hepatocarcinogenic compound of all of the aflatoxins. In this report, we explore the capacity of β-D-glucan (Glu) to reduce the DNA damage induced by AFB1 in mouse hepatocytes. For this purpose, we applied the comet assay to groups of animals that were first administered Glu in three doses (100, 400 and 700 mg/kg bw, respectively) and, 20 min later, 1.0 mg/kg of AFB1. Liver cells were obtained at 4, 10 and 16 h after the chemical administration and examined. The results showed no protection of the damage induced byAFB1 with the low dose of the polysaccharide, but they did reveal antigenotoxic activity exerted by the two high doses. In addition, we induced a co-crystallization between both compounds, determined their fusion points and analyzed the molecules by UV spectroscopy. The data suggested the formation of a supramolecular complex between AFB1 and β-D-glucan.
KW - Aflatoxin B
KW - Antigenotoxicity
KW - Glucan
KW - Mouse hepatocytes
UR - http://www.scopus.com/inward/record.url?scp=84934963548&partnerID=8YFLogxK
U2 - 10.3390/toxins7062145
DO - 10.3390/toxins7062145
M3 - Artículo
C2 - 26110504
SN - 2072-6651
VL - 7
SP - 2145
EP - 2158
JO - Toxins
JF - Toxins
IS - 6
ER -