© 2018 International Society for Chemotherapy of Infection and Cancer Objectives: The aim of this study was to determinate the prevalence of Escherichia coli and its resistance to antimicrobials and the presence of virulence genes in retail samples of beef and pork in several locations in Tamaulipas, Mexico. Methods: A total of 106 samples (54 beef and 52 pork) collected from August 2013 to March 2014 were analysed to detect E. coli isolates. The E. coli isolates were then analysed for detection of virulence factors and antimicrobial resistance genes. Antimicrobial susceptibility to 16 antimicrobial agents was also determined. Results: A total of 158 E. coli isolates were obtained, among which 3 (1.9%) harboured the virulence gene stx1, 28 (17.7%) harboured stx2 and 34 (21.5%) harboured hlyA. High phenotypic resistance was observed in almost all isolates, since 146 (92.4%) showed a multiresistant phenotype with resistance to cefalotin (92%), ampicillin (92%), cefotaxime (78%), nitrofurantoin (76%) and tetracycline (75%). The antimicrobial resistance genes tet(A) and tet(B) were detected in 56% of isolates, strA in 9.6%, aadA in 17% and aac(3)-IV in only 0.6% of strains. Conclusions: Based on these results, it can be concluded that retail beef and pork meat may play a role in the spread of antimicrobial-resistant E. coli strains in this region.