TY - JOUR
T1 - Control poscosecha de rhizopus stolonifer en zarzamora (Rubus fruticosus) por bacterias nativas de zarzamora
AU - Chavez-Diaz, Ismael F.
AU - Mena-Violante, Hortencia G.
AU - Hernandez-Lauzardo, Ana N.
AU - Oyoque-Salcedo, Guadalupe
AU - Oregel-Zamudio, Ernesto
AU - Angoa-Perez, M. Valentina
N1 - Publisher Copyright:
© 2019, Universidad Nacional de Cuyo. All rights reserved.
PY - 2019/7/1
Y1 - 2019/7/1
N2 - The potential of four native bacterial strains of blackberries cv. Brazos (Rubus fruticosus): Bacillus subtilis (BSS), Bacillus subtilis (BSL), Bacillus licheniformis (BLI) and Leifsonia aquatica (LAQ), was evaluated for the postharvest control of soft rot caused by Rhizopus stolonifer in blackberry fruits. The fruits were treated with cell suspensions (CS) and cell-free supernatants (CFE) from each bacterial strain and were infected with two strains of R. stolonifer (RSA and RSC). The severity and inhibition percentage of the disease were determined. Additionally, the inhibition by siderophores and the inhibition percentage of R. stolonifer spore germination were analyzed as possible control mechanisms. The CS of BSS inhibited RSA by 45.8%, followed by CFE of LAQ which controlled the same strain by 39.7%. The CS of BLI inhibited RSC by 37.7%, whereas the CFE of BSS and LAQ controlled it by 47.7 and 41.8%, respectively. All bacterial strains inhibited RSA and RSC by siderophores production (38.7 to 48.6 %) and the inhibition of spore germination of RSC was higher than 93% after 48 h. This work is one of the first to report R. stolonifer control by native bacteria CS and CFE, particularly LAQ in postharvested blackberry fruits. These results show the combination of mechanisms used by bacteria to control both R. stolonifer strains.
AB - The potential of four native bacterial strains of blackberries cv. Brazos (Rubus fruticosus): Bacillus subtilis (BSS), Bacillus subtilis (BSL), Bacillus licheniformis (BLI) and Leifsonia aquatica (LAQ), was evaluated for the postharvest control of soft rot caused by Rhizopus stolonifer in blackberry fruits. The fruits were treated with cell suspensions (CS) and cell-free supernatants (CFE) from each bacterial strain and were infected with two strains of R. stolonifer (RSA and RSC). The severity and inhibition percentage of the disease were determined. Additionally, the inhibition by siderophores and the inhibition percentage of R. stolonifer spore germination were analyzed as possible control mechanisms. The CS of BSS inhibited RSA by 45.8%, followed by CFE of LAQ which controlled the same strain by 39.7%. The CS of BLI inhibited RSC by 37.7%, whereas the CFE of BSS and LAQ controlled it by 47.7 and 41.8%, respectively. All bacterial strains inhibited RSA and RSC by siderophores production (38.7 to 48.6 %) and the inhibition of spore germination of RSC was higher than 93% after 48 h. This work is one of the first to report R. stolonifer control by native bacteria CS and CFE, particularly LAQ in postharvested blackberry fruits. These results show the combination of mechanisms used by bacteria to control both R. stolonifer strains.
KW - Bacillus
KW - Biocontrol
KW - Cell-free Extract
KW - Leifsonia
KW - Postharvest berries
KW - Rhizopus stolonifera
KW - Soft rot
UR - http://www.scopus.com/inward/record.url?scp=85076735456&partnerID=8YFLogxK
M3 - Artículo
AN - SCOPUS:85076735456
SN - 0370-4661
VL - 51
SP - 360
EP - 317
JO - Revista de la Facultad de Ciencias Agrarias
JF - Revista de la Facultad de Ciencias Agrarias
IS - 2
ER -