Platelet lysate-loaded PLGA nanoparticles in a thermo-responsive hydrogel intended for the treatment of wounds

Sergio Alberto Bernal-Chávez, Sergio Alcalá-Alcalá, Doris Cerecedo, Adriana Ganem-Rondero

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

A thermo-responsive hydrogel of Pluronic F-127, containing PLGA nanoparticles loaded with a platelet lysate for wound treatment, was prepared. A high rate of incorporation of the lysate (about 80%) in the nanoparticles was achieved by the double emulsion-solvent evaporation method. The nanoparticles were characterized by measuring their size (about 318 nm), polydispersity index (0.29) and Z potential (-17.6), as well as by infrared and calorimetric techniques, and determining their stability as a function of time. It was found through measures of transepidermal water loss that the hydrogel containing the nanoparticles was capable of providing a semi-occlusive environment, necessary for the recovery of a wound. The inclusion of lysate in nanoparticles and this in turn in the hydrogel allowed a gradual release, which would avoid contact of the total dose with the biological medium. Studies with fibroblasts and in vivo in mice showed that the hydrogel containing nanoparticles with platelet lysate promoted faster tissue regeneration than the lysate in its free form, so this system is presented as a good alternative for the treatment of wounds.

Original languageEnglish
Article number105231
JournalEuropean Journal of Pharmaceutical Sciences
Volume146
DOIs
StatePublished - 15 Apr 2020

Keywords

  • Hydrogel
  • PLGA nanoparticles
  • Platelet lysate
  • Pluronic F-127
  • Thermo-responsive polymer
  • Wound healing

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