© 2016 Universidad Autonoma de Tlaxcala. All right reserved. The improper disposal of organic wastes is an environmental problem. An alternative to this problems is to use the combined anaerobic digestion (co-AD), in which organic matter is degraded by microorganisms to produce biogas and digestate, which can be used as fertilizer. Digestate comprises a liquid fraction (biol) and a solid one. In this work the phytotoxicity of bioles obtained from different conditions of co-AD was evaluated. Four batch type biodigesters tratments and a control were prepared, all at 70% capacity, 10% solids dry basis, retention time of 60 days and mesophilic temperature conditions. The treatments consisted in 80% residue of vegetables and 20% pig manure, a group of digesters was adjusted to initial pH 6 and another initial pH 8, and with or without adjusting the Carbon:Nitrogen ratio to 20:1. PH was monitored during the process. Phytotoxicity test was done through germination index (GI). GI was calculated to the bioles of weeks 3, 6 and 8 of the process of co-DA. Six bioles obtained in different weeks were not phytotoxic, the bioles obtained from co-DA where the initial pH was adjusted to 8 were growth promoters in week 6; however, the control obtained higher GI than the treatments.