TY - JOUR
T1 - Phytochemical screening and anti-inflammatory potential of the organic extracts from cleoserrata serrata (Jacq.) iltis
AU - Del Carmen Juárez-Vázquez, María
AU - Zamilpa A, Alejandro
AU - León-Díaz, Rosalba
AU - Martínez-Vázquez, Mariano
AU - López-Torres, Adolfo
AU - Luna-Herrera, Julieta
AU - Yépez-Mulia, Lilian
AU - Alarcón-Aguilar, Francisco
AU - Jiménez-Arellanes, María Adelina
N1 - Publisher Copyright:
© 2021 Phcogj.Com.
PY - 2021/9
Y1 - 2021/9
N2 - Cleoserrata serrata is used in Mexican-south traditional medicine to treat chicleros ulcer. The phytochemical profile and the anti-inflammatory effect from four extracts obtained by maceration proccess and of the primary fractions from two extracts are described. In addition, the antioxidant, leishmanicidal and antimycobaterial activities and LD50 from active extracts are reported. Anti-inflammatory activity was evaluated in TPA and carrageenan assays. Ethyl palmitate and ?-sitosterol were detected in Hexanic and CH2Cl2 extracts. Same compounds and stigmasta-3,5-dien-7-one, palmitic acid, phytol acetate and phytol were detected in primary fractions from CH2Cl2:EtOH extract. In this and in MeOH extracts a polyphenolmixture was obtained. The MeOH extract was subjected to acid hydrolysis, and kaempferol, quercetin and scopoletin were detected in organic-phase. Polyphenol-mixture and organic-phase (IC50=3730 and 2338 μg/mL) showed moderate antioxidant activity; meanwhile MeOH extract exhibited scarce activity. In carrageenan model, Hexanic extract and polyphenol-mixture showed ED50=131.46 and 64.89 mg/ kg, respectively. Three extracts were active but not-dose-dependent. In TPA-model, CH2Cl2 extract and polyphenol-mixture showed ED50 <0.79 mg/ear, and three extracts were active, however the effect was not-dose-dependent. CH2Cl2:EtOH showed antimycobacterial and leishmanicidal activities. The LD50 was >2 g/kg for all extracts.
AB - Cleoserrata serrata is used in Mexican-south traditional medicine to treat chicleros ulcer. The phytochemical profile and the anti-inflammatory effect from four extracts obtained by maceration proccess and of the primary fractions from two extracts are described. In addition, the antioxidant, leishmanicidal and antimycobaterial activities and LD50 from active extracts are reported. Anti-inflammatory activity was evaluated in TPA and carrageenan assays. Ethyl palmitate and ?-sitosterol were detected in Hexanic and CH2Cl2 extracts. Same compounds and stigmasta-3,5-dien-7-one, palmitic acid, phytol acetate and phytol were detected in primary fractions from CH2Cl2:EtOH extract. In this and in MeOH extracts a polyphenolmixture was obtained. The MeOH extract was subjected to acid hydrolysis, and kaempferol, quercetin and scopoletin were detected in organic-phase. Polyphenol-mixture and organic-phase (IC50=3730 and 2338 μg/mL) showed moderate antioxidant activity; meanwhile MeOH extract exhibited scarce activity. In carrageenan model, Hexanic extract and polyphenol-mixture showed ED50=131.46 and 64.89 mg/ kg, respectively. Three extracts were active but not-dose-dependent. In TPA-model, CH2Cl2 extract and polyphenol-mixture showed ED50 <0.79 mg/ear, and three extracts were active, however the effect was not-dose-dependent. CH2Cl2:EtOH showed antimycobacterial and leishmanicidal activities. The LD50 was >2 g/kg for all extracts.
KW - Anti-inflammatory effect
KW - Antimycobacterial activity
KW - Antioxidant activity
KW - Cleoserrata serrata
KW - DL
KW - Leishmanicidal activity
KW - Organic extract
UR - http://www.scopus.com/inward/record.url?scp=85115410404&partnerID=8YFLogxK
U2 - 10.5530/pj.2021.13.156
DO - 10.5530/pj.2021.13.156
M3 - Artículo
AN - SCOPUS:85115410404
SN - 0975-3575
VL - 13
SP - 1225
EP - 1241
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 5
ER -