TY - JOUR
T1 - Physiological and pathogenical characterization of beauveria bassiana and metarhizium anisopliae isolates for management of adult spodoptera frugiperda
AU - Gutiérrez-Cárdenas, Oscar Giovanni
AU - Cortez-Madrigal, Hipolito
AU - Malo, Edi A.
AU - Gómez-Ruíz, Jaime
AU - Nord, Rineaud
N1 - Publisher Copyright:
© 2019 Southwestern Entomological Society. All rights reserved.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - To know the potential of entomopathogenic fungi for management of adults of Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) using the auto-dissemination technique, three isolates of Beauveria bassiana (Balsamo-Crivelli) Vuillemin and six isolates of Metarhizium anisopliae (Metschnikoff) (Hypocreales: Clavicipitaceae) were characterized physiologically and pathogenically. At the time 50% of conidia germinated (GT50), pathogenicity, effect of relative humidity, and horizontal transmission of the entomopathogen male-female in three ratios (1:5, 1:10, 1:15) were evaluated. In addition, a preliminary device for auto-dissemination of the entomopathogen was evaluated. All isolates were pathogenic (33.3-100%) toward adults of the insect. Pathogenicity and TG50 were very correlated (r = 0.8) and fluctuated from 14.1 to 20.3 hours. Because of intermediate pathogenicity (68.7%) and varying response to humidity (62-100%), isolation of M. anisopliae Ma-San Rafel-2 was used for auto-dissemination bioassays. Transmission of the entomopathogen was a function of the number of females exposed. After 11 days, a male transmitted the fungus to 15 healthy females. The males released in devices prepared with fungus + synthetic sex pheromone were attracted, infected, and killed 5 to 8 days later. Our results are fundamental for management of S. frugiperda through auto-dissemination of entomopathogenic fungi.
AB - To know the potential of entomopathogenic fungi for management of adults of Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) using the auto-dissemination technique, three isolates of Beauveria bassiana (Balsamo-Crivelli) Vuillemin and six isolates of Metarhizium anisopliae (Metschnikoff) (Hypocreales: Clavicipitaceae) were characterized physiologically and pathogenically. At the time 50% of conidia germinated (GT50), pathogenicity, effect of relative humidity, and horizontal transmission of the entomopathogen male-female in three ratios (1:5, 1:10, 1:15) were evaluated. In addition, a preliminary device for auto-dissemination of the entomopathogen was evaluated. All isolates were pathogenic (33.3-100%) toward adults of the insect. Pathogenicity and TG50 were very correlated (r = 0.8) and fluctuated from 14.1 to 20.3 hours. Because of intermediate pathogenicity (68.7%) and varying response to humidity (62-100%), isolation of M. anisopliae Ma-San Rafel-2 was used for auto-dissemination bioassays. Transmission of the entomopathogen was a function of the number of females exposed. After 11 days, a male transmitted the fungus to 15 healthy females. The males released in devices prepared with fungus + synthetic sex pheromone were attracted, infected, and killed 5 to 8 days later. Our results are fundamental for management of S. frugiperda through auto-dissemination of entomopathogenic fungi.
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U2 - 10.3958/059.044.0206
DO - 10.3958/059.044.0206
M3 - Artículo
AN - SCOPUS:85068366300
SN - 0147-1724
VL - 44
SP - 409
EP - 421
JO - Southwestern Entomologist
JF - Southwestern Entomologist
IS - 2
ER -