TY - JOUR
T1 - Physico-chemical, nutritional and infrared spectroscopy evaluation of an optimized soybean/corn flour extrudate
AU - Guzmán-Ortiz, Fabiola Araceli
AU - Hernández-Sánchez, Humberto
AU - Yee-Madeira, Hernani
AU - Martín-Martínez, Eduardo San
AU - Robles-Ramírez, María del Carmen
AU - Rojas-López, Marlon
AU - Berríos, Jose De J.
AU - Mora-Escobedo, Rosalva
N1 - Publisher Copyright:
© Association of Food Scientists & Technologists (India) 2014.
PY - 2015/7
Y1 - 2015/7
N2 - A central composite design using RMS (Response Surface Methodology) successfully described the effect of independent variables (feed moisture, die temperature and soybean proportion) on the specific parameters of product quality as expansion index (EI), water absorption index (WAI), water solubility index (WSI) and total color difference (ΔE) studied. The regression model indicated that EI, WAI, WSI and ΔE were significant (p<0.05) with coefficients of determination (R2) of 0.7371, 0.7588, 0.7622, 0.8150, respectively. The optimized processing conditions were obtained with 25.8 % feed moisture, 160 °C die temperature and 58 %/42 % soybean/corn proportion. It was not found statistically changes in amino acid profile due to extrusion process. The electrophoretic profile of extruded soybean/corn mix presented low intensity molecular weight bands, compared to the unprocessed sample. The generation of low molecular weight polypeptides was associated to an increased in In vitro protein digestibility (IVPD) of the extrudate. The FTIR spectra of the soybean/corn mix before and after extrusion showed that the α-helix structure remained unchanged after extrusion. However, the band associated with β-sheet structure showed to be split into two bands at 1624 and 1640 cm−1 . The changes in the β-sheet structures may be also associated to the increased in IVPD in the extruded sample.
AB - A central composite design using RMS (Response Surface Methodology) successfully described the effect of independent variables (feed moisture, die temperature and soybean proportion) on the specific parameters of product quality as expansion index (EI), water absorption index (WAI), water solubility index (WSI) and total color difference (ΔE) studied. The regression model indicated that EI, WAI, WSI and ΔE were significant (p<0.05) with coefficients of determination (R2) of 0.7371, 0.7588, 0.7622, 0.8150, respectively. The optimized processing conditions were obtained with 25.8 % feed moisture, 160 °C die temperature and 58 %/42 % soybean/corn proportion. It was not found statistically changes in amino acid profile due to extrusion process. The electrophoretic profile of extruded soybean/corn mix presented low intensity molecular weight bands, compared to the unprocessed sample. The generation of low molecular weight polypeptides was associated to an increased in In vitro protein digestibility (IVPD) of the extrudate. The FTIR spectra of the soybean/corn mix before and after extrusion showed that the α-helix structure remained unchanged after extrusion. However, the band associated with β-sheet structure showed to be split into two bands at 1624 and 1640 cm−1 . The changes in the β-sheet structures may be also associated to the increased in IVPD in the extruded sample.
KW - Amino acid profile and electrophoresis
KW - Extrusion cooking of soybean/corn
KW - In vitro protein digestibility
KW - Infra-red spectroscopy (FTIR)
KW - Protein changes
KW - Response surface methodology
UR - http://www.scopus.com/inward/record.url?scp=85027926116&partnerID=8YFLogxK
U2 - 10.1007/s13197-014-1485-5
DO - 10.1007/s13197-014-1485-5
M3 - Artículo
C2 - 26139872
SN - 0022-1155
VL - 52
SP - 4066
EP - 4077
JO - Journal of Food Science and Technology
JF - Journal of Food Science and Technology
IS - 7
M1 - A011
ER -