Pharmacological and genotoxic properties of polyphenolic extracts of Cedrela odorata L. and Juglans regia L. barks in rodents

Dulce Carolina Almonte-Flores, Norma Paniagua-Castro, Gerardo Escalona-Cardoso, Martha Rosales-Castro

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

© 2015 Dulce Carolina Almonte-Flores et al. Evaluation of the phenolic compounds and antioxidant activity of Cedrela odorata L. and Juglans regia L. bark extracts was performed in vitro. Juglans regia showed greater extract concentration and higher antioxidant activity. Hypoglycemic activity in rats was assessed by generating a glucose tolerance curve and determining the area under the curve (AUC). Diabetes was later induced by an injection with streptozotocin (65 mg/kg of b.w.) and confirmed after 24 hours. The extract was administered (200 mg/kg b.w.) over 10 days, and blood glucose was monitored and compared with a control group. The glucose AUC showed a hypoglycemic effect of J. regia and C. odorata in normal rats. Both extracts reduced hepatic lipid peroxidation in diabetic rats. Polyphenolic extracts reduced cholesterol levels in a hypercholesterolemic mouse model and decreased hepatic lipid peroxidation. Polyphenolic extract doses of 100 and 200 mg/kg b.w. were administered alone or with cyclophosphamide (CPA) 50 mg/kg ip, which was used as a positive control. Analyses were performed using leukocytes in a comet assay after 4 and 24 h of treatment. Genotoxic effects were evaluated by the comet assay, which showed that while J. regia extract had no effect, C. odorata extract induced slight damage at 200 mg/kg, with the formation of type 0 and 1 comets.
Original languageAmerican English
JournalEvidence-based Complementary and Alternative Medicine
DOIs
StatePublished - 1 Jan 2015

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Cedrela
Juglans
Rodentia
Pharmacology
Comet Assay
Hypoglycemic Agents
Lipid Peroxidation
Area Under Curve
Antioxidants
Glucose
Liver
Streptozocin
Cyclophosphamide
Blood Glucose
Leukocytes
Cholesterol
Control Groups
Injections

Cite this

@article{4887aaa058b74431a8556fdaa095e368,
title = "Pharmacological and genotoxic properties of polyphenolic extracts of Cedrela odorata L. and Juglans regia L. barks in rodents",
abstract = "{\circledC} 2015 Dulce Carolina Almonte-Flores et al. Evaluation of the phenolic compounds and antioxidant activity of Cedrela odorata L. and Juglans regia L. bark extracts was performed in vitro. Juglans regia showed greater extract concentration and higher antioxidant activity. Hypoglycemic activity in rats was assessed by generating a glucose tolerance curve and determining the area under the curve (AUC). Diabetes was later induced by an injection with streptozotocin (65 mg/kg of b.w.) and confirmed after 24 hours. The extract was administered (200 mg/kg b.w.) over 10 days, and blood glucose was monitored and compared with a control group. The glucose AUC showed a hypoglycemic effect of J. regia and C. odorata in normal rats. Both extracts reduced hepatic lipid peroxidation in diabetic rats. Polyphenolic extracts reduced cholesterol levels in a hypercholesterolemic mouse model and decreased hepatic lipid peroxidation. Polyphenolic extract doses of 100 and 200 mg/kg b.w. were administered alone or with cyclophosphamide (CPA) 50 mg/kg ip, which was used as a positive control. Analyses were performed using leukocytes in a comet assay after 4 and 24 h of treatment. Genotoxic effects were evaluated by the comet assay, which showed that while J. regia extract had no effect, C. odorata extract induced slight damage at 200 mg/kg, with the formation of type 0 and 1 comets.",
author = "Almonte-Flores, {Dulce Carolina} and Norma Paniagua-Castro and Gerardo Escalona-Cardoso and Martha Rosales-Castro",
year = "2015",
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doi = "10.1155/2015/187346",
language = "American English",
journal = "Evidence-based Complementary and Alternative Medicine",
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Pharmacological and genotoxic properties of polyphenolic extracts of Cedrela odorata L. and Juglans regia L. barks in rodents. / Almonte-Flores, Dulce Carolina; Paniagua-Castro, Norma; Escalona-Cardoso, Gerardo; Rosales-Castro, Martha.

In: Evidence-based Complementary and Alternative Medicine, 01.01.2015.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Pharmacological and genotoxic properties of polyphenolic extracts of Cedrela odorata L. and Juglans regia L. barks in rodents

AU - Almonte-Flores, Dulce Carolina

AU - Paniagua-Castro, Norma

AU - Escalona-Cardoso, Gerardo

AU - Rosales-Castro, Martha

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N2 - © 2015 Dulce Carolina Almonte-Flores et al. Evaluation of the phenolic compounds and antioxidant activity of Cedrela odorata L. and Juglans regia L. bark extracts was performed in vitro. Juglans regia showed greater extract concentration and higher antioxidant activity. Hypoglycemic activity in rats was assessed by generating a glucose tolerance curve and determining the area under the curve (AUC). Diabetes was later induced by an injection with streptozotocin (65 mg/kg of b.w.) and confirmed after 24 hours. The extract was administered (200 mg/kg b.w.) over 10 days, and blood glucose was monitored and compared with a control group. The glucose AUC showed a hypoglycemic effect of J. regia and C. odorata in normal rats. Both extracts reduced hepatic lipid peroxidation in diabetic rats. Polyphenolic extracts reduced cholesterol levels in a hypercholesterolemic mouse model and decreased hepatic lipid peroxidation. Polyphenolic extract doses of 100 and 200 mg/kg b.w. were administered alone or with cyclophosphamide (CPA) 50 mg/kg ip, which was used as a positive control. Analyses were performed using leukocytes in a comet assay after 4 and 24 h of treatment. Genotoxic effects were evaluated by the comet assay, which showed that while J. regia extract had no effect, C. odorata extract induced slight damage at 200 mg/kg, with the formation of type 0 and 1 comets.

AB - © 2015 Dulce Carolina Almonte-Flores et al. Evaluation of the phenolic compounds and antioxidant activity of Cedrela odorata L. and Juglans regia L. bark extracts was performed in vitro. Juglans regia showed greater extract concentration and higher antioxidant activity. Hypoglycemic activity in rats was assessed by generating a glucose tolerance curve and determining the area under the curve (AUC). Diabetes was later induced by an injection with streptozotocin (65 mg/kg of b.w.) and confirmed after 24 hours. The extract was administered (200 mg/kg b.w.) over 10 days, and blood glucose was monitored and compared with a control group. The glucose AUC showed a hypoglycemic effect of J. regia and C. odorata in normal rats. Both extracts reduced hepatic lipid peroxidation in diabetic rats. Polyphenolic extracts reduced cholesterol levels in a hypercholesterolemic mouse model and decreased hepatic lipid peroxidation. Polyphenolic extract doses of 100 and 200 mg/kg b.w. were administered alone or with cyclophosphamide (CPA) 50 mg/kg ip, which was used as a positive control. Analyses were performed using leukocytes in a comet assay after 4 and 24 h of treatment. Genotoxic effects were evaluated by the comet assay, which showed that while J. regia extract had no effect, C. odorata extract induced slight damage at 200 mg/kg, with the formation of type 0 and 1 comets.

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