TY - JOUR
T1 - Pharmacokinetics of diphenylboroxazolidones of L-α-amino acids with activity on the CNS
T2 - Quantification in rat DBS by UPLC-MS/MS
AU - González, Alberto Garcáa
AU - Zavala, Leticia Contreras
AU - Moreno, Alionka P.Angeles
AU - San Juan, Eduardo Ramárez
AU - Ferrara, José G.Trujillo
AU - Espinosa, Liliana Rivera
AU - Jiméez, Gabriel Marcelán
PY - 2011/2
Y1 - 2011/2
N2 - Background: A growing number of boron-containing compounds exhibit many important biological activities; of particular interest are the αamino acid borinic derivatives with activity in the CNS. A validated, sensitive and specific UPLC-MS/MS technique for quantification of the diphenylboroxazolidones of glycine (DBPX-gly), L-aspartate (DPBX-L-asp) and L-glutamate (DPBX-L-glu) in dried blood spots (DBSs) is presented. Results: The most intense signal corresponds to compounds with 11B. The extraction procedure was liquid elution of 3.2-mm punched DBSs with acetonitrile:aqueous formic acid 0.1% (80:20 v/v). Assays proved to be linear, falling accurately and precisely within the range of 0.3-50 μg/ml for DPBX-L-asp and DPBX-L-glu and 0.1-5 μg/ml for DBPX-gly. Chromatograms exhibit clean 2.0-min running time peaks and S/N ratios for the LLOQ were approximately 15:1. The technique was used to evaluate the pharmacokinetics of the molecules and to correlate these with timecourse toxic effects. Conclusion: DBSs represent an advantage for the collection of small volumes of samples, and also in terms of processing and storage. UPLC-MS/MS allow us not only to identify the isotopic pattern of boron in DBPX, but also to quantify them with accuracy and specificity. Pharmacokinetics of these molecules exhibit a high apparent volume of distribution; it suggests a preference of DPBX-amino acids for fatty tissues such as the CNS.
AB - Background: A growing number of boron-containing compounds exhibit many important biological activities; of particular interest are the αamino acid borinic derivatives with activity in the CNS. A validated, sensitive and specific UPLC-MS/MS technique for quantification of the diphenylboroxazolidones of glycine (DBPX-gly), L-aspartate (DPBX-L-asp) and L-glutamate (DPBX-L-glu) in dried blood spots (DBSs) is presented. Results: The most intense signal corresponds to compounds with 11B. The extraction procedure was liquid elution of 3.2-mm punched DBSs with acetonitrile:aqueous formic acid 0.1% (80:20 v/v). Assays proved to be linear, falling accurately and precisely within the range of 0.3-50 μg/ml for DPBX-L-asp and DPBX-L-glu and 0.1-5 μg/ml for DBPX-gly. Chromatograms exhibit clean 2.0-min running time peaks and S/N ratios for the LLOQ were approximately 15:1. The technique was used to evaluate the pharmacokinetics of the molecules and to correlate these with timecourse toxic effects. Conclusion: DBSs represent an advantage for the collection of small volumes of samples, and also in terms of processing and storage. UPLC-MS/MS allow us not only to identify the isotopic pattern of boron in DBPX, but also to quantify them with accuracy and specificity. Pharmacokinetics of these molecules exhibit a high apparent volume of distribution; it suggests a preference of DPBX-amino acids for fatty tissues such as the CNS.
UR - http://www.scopus.com/inward/record.url?scp=79952011639&partnerID=8YFLogxK
U2 - 10.4155/bio.10.208
DO - 10.4155/bio.10.208
M3 - Artículo
C2 - 21338263
SN - 1757-6180
VL - 3
SP - 439
EP - 448
JO - Bioanalysis
JF - Bioanalysis
IS - 4
ER -